Ahniella affigens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Ahniella

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4694 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1PPF9|A0A2P1PPF9_9GAMM GspH domain-containing protein OS=Ahniella affigens OX=2021234 GN=C7S18_05660 PE=4 SV=1
MM1 pKa = 7.21MRR3 pKa = 11.84RR4 pKa = 11.84ADD6 pKa = 4.08ADD8 pKa = 3.53QCPVPSGLTKK18 pKa = 10.67LRR20 pKa = 11.84TLLWIGVCGLSACAHH35 pKa = 5.47TPKK38 pKa = 10.2PDD40 pKa = 3.63ADD42 pKa = 3.67GAGANSAVRR51 pKa = 11.84SPAPVTATADD61 pKa = 3.38LGQPMSVQTEE71 pKa = 4.03ALAVQQSNGSAQYY84 pKa = 10.69QRR86 pKa = 11.84LFPARR91 pKa = 11.84TDD93 pKa = 3.42TQPQFQVPHH102 pKa = 6.29ARR104 pKa = 11.84WPVLPGTRR112 pKa = 11.84GRR114 pKa = 11.84GNASAPEE121 pKa = 3.96RR122 pKa = 11.84VQGGGAAVAEE132 pKa = 4.46ATAGSPSDD140 pKa = 3.55RR141 pKa = 11.84PTAVGDD147 pKa = 3.33AAKK150 pKa = 9.91IVGSIAPKK158 pKa = 9.76FDD160 pKa = 4.97SIAFDD165 pKa = 5.39DD166 pKa = 4.98NGTLNDD172 pKa = 3.68GFRR175 pKa = 11.84FIPADD180 pKa = 3.22AHH182 pKa = 6.3AAAGPNHH189 pKa = 5.69VVAVSNVTLKK199 pKa = 10.05IHH201 pKa = 6.61RR202 pKa = 11.84KK203 pKa = 9.58SDD205 pKa = 3.39GQVQLQTGLQDD216 pKa = 3.62FFAGLGSQRR225 pKa = 11.84PSTYY229 pKa = 10.07TFDD232 pKa = 3.59PRR234 pKa = 11.84VLFDD238 pKa = 3.63PFEE241 pKa = 4.07NRR243 pKa = 11.84FVVLTLEE250 pKa = 4.46VLSQANGDD258 pKa = 3.76ATDD261 pKa = 3.49SSFLFVAVSDD271 pKa = 4.23DD272 pKa = 3.8ADD274 pKa = 4.06PNGVWRR280 pKa = 11.84MARR283 pKa = 11.84INTKK287 pKa = 8.92LTISSTPGWLDD298 pKa = 3.35YY299 pKa = 10.99PGFGIDD305 pKa = 3.83EE306 pKa = 4.7DD307 pKa = 4.03ALYY310 pKa = 9.39VTGNYY315 pKa = 9.85FDD317 pKa = 3.98FTVGNYY323 pKa = 9.91LDD325 pKa = 3.57SRR327 pKa = 11.84LWILNKK333 pKa = 10.08GVSGGLYY340 pKa = 10.21SGGTVVARR348 pKa = 11.84QFDD351 pKa = 4.52PEE353 pKa = 4.1PTLADD358 pKa = 3.72SAPVMPARR366 pKa = 11.84MLSAGPGTVGTWLAAYY382 pKa = 10.23SGFATTGGVEE392 pKa = 4.25QINTIRR398 pKa = 11.84IDD400 pKa = 3.61NPLNTSPTFTSALEE414 pKa = 3.99NVADD418 pKa = 3.83IDD420 pKa = 4.02QSFTVDD426 pKa = 4.73FPNAPQPGSVAGLDD440 pKa = 3.43PGDD443 pKa = 3.65RR444 pKa = 11.84RR445 pKa = 11.84MLDD448 pKa = 3.59VVWHH452 pKa = 5.75NNRR455 pKa = 11.84LSMVFNVLPPASQSNANQNTAYY477 pKa = 9.54WLQLDD482 pKa = 3.92TSVLNNLSVAQRR494 pKa = 11.84GQIGGEE500 pKa = 3.88DD501 pKa = 3.8LGSSVHH507 pKa = 5.56TFYY510 pKa = 10.9PSIATNQFGHH520 pKa = 6.92LAISFAASSTNLNPGAYY537 pKa = 9.16VVTRR541 pKa = 11.84RR542 pKa = 11.84PTDD545 pKa = 3.35SNNTVSSSQTLRR557 pKa = 11.84AGLDD561 pKa = 3.13TYY563 pKa = 11.5LRR565 pKa = 11.84TFGGNRR571 pKa = 11.84NRR573 pKa = 11.84WGDD576 pKa = 3.57YY577 pKa = 9.46SATMVDD583 pKa = 3.72PTDD586 pKa = 3.26QCFWSFNLWSNTNGTVISGEE606 pKa = 4.01DD607 pKa = 3.86GRR609 pKa = 11.84WSTTAGRR616 pKa = 11.84LCTCEE621 pKa = 4.16GTEE624 pKa = 4.17STGDD628 pKa = 3.21GDD630 pKa = 4.65FDD632 pKa = 3.8GWCNSLDD639 pKa = 3.72NCDD642 pKa = 4.88LISNRR647 pKa = 11.84DD648 pKa = 3.56QLDD651 pKa = 3.77PDD653 pKa = 3.93SDD655 pKa = 4.47TVGTACDD662 pKa = 3.63NCPNAANTNQADD674 pKa = 3.75GDD676 pKa = 3.95NDD678 pKa = 3.65QVGTVCDD685 pKa = 3.63NCSTVANTNQANADD699 pKa = 3.12ADD701 pKa = 3.78ARR703 pKa = 11.84GDD705 pKa = 3.61VCDD708 pKa = 3.61NCVNVANNDD717 pKa = 3.9QINSDD722 pKa = 3.68ADD724 pKa = 4.01SVGDD728 pKa = 3.5ACDD731 pKa = 3.31NCRR734 pKa = 11.84LISNQNQSNLDD745 pKa = 3.5FDD747 pKa = 4.36QLGDD751 pKa = 3.73ACDD754 pKa = 3.75NCPFAANNDD763 pKa = 3.83QLNSDD768 pKa = 3.73TDD770 pKa = 4.07GLGNACDD777 pKa = 3.74NCPLVTNQSQINSDD791 pKa = 3.51SDD793 pKa = 4.1LLGDD797 pKa = 4.44ACDD800 pKa = 3.71NCISVANASQTNSDD814 pKa = 3.62SDD816 pKa = 4.31LLGDD820 pKa = 4.56ACDD823 pKa = 3.79NCDD826 pKa = 5.29LISNPDD832 pKa = 3.35QADD835 pKa = 3.36GDD837 pKa = 4.06GDD839 pKa = 4.44LVGTACDD846 pKa = 3.47NCPAAGNTNQTNSDD860 pKa = 3.98GDD862 pKa = 3.93PLGDD866 pKa = 3.46VCDD869 pKa = 3.86NCVTVTNPDD878 pKa = 3.44QLNTDD883 pKa = 3.49MDD885 pKa = 4.62SLGDD889 pKa = 3.76ACDD892 pKa = 4.26GDD894 pKa = 4.6DD895 pKa = 5.93DD896 pKa = 5.13NDD898 pKa = 3.93AVPDD902 pKa = 4.0GTDD905 pKa = 3.19NCPLVPNLNQTNTDD919 pKa = 3.45GANDD923 pKa = 4.3GGDD926 pKa = 3.39ACDD929 pKa = 4.86PDD931 pKa = 5.27DD932 pKa = 6.77DD933 pKa = 5.29NDD935 pKa = 4.11TVLDD939 pKa = 4.31GVDD942 pKa = 3.54NCPLIVNLNQANADD956 pKa = 3.43GDD958 pKa = 4.22ALGDD962 pKa = 3.67VCDD965 pKa = 5.31PDD967 pKa = 5.26DD968 pKa = 6.29DD969 pKa = 5.07NDD971 pKa = 3.97GFQDD975 pKa = 4.5ASDD978 pKa = 3.82NCPLVSNVDD987 pKa = 3.6QLDD990 pKa = 3.81DD991 pKa = 5.48DD992 pKa = 4.82GDD994 pKa = 3.89LRR996 pKa = 11.84GNVCDD1001 pKa = 3.74NCPAISNADD1010 pKa = 3.4QADD1013 pKa = 3.76RR1014 pKa = 11.84DD1015 pKa = 3.49LDD1017 pKa = 3.61QRR1019 pKa = 11.84GNVCDD1024 pKa = 3.82NCPFAANPSQQDD1036 pKa = 3.35DD1037 pKa = 4.05DD1038 pKa = 5.37GDD1040 pKa = 4.68LIGNVCDD1047 pKa = 4.5FCPNEE1052 pKa = 4.45FSNQCYY1058 pKa = 9.8VFGDD1062 pKa = 4.47GYY1064 pKa = 11.1EE1065 pKa = 4.56DD1066 pKa = 4.64PLTLL1070 pKa = 4.98

Molecular weight: 112.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1PLR2|A0A2P1PLR2_9GAMM Alpha/beta hydrolase OS=Ahniella affigens OX=2021234 GN=C7S18_00605 PE=4 SV=1
MM1 pKa = 7.49GKK3 pKa = 7.8TRR5 pKa = 11.84AEE7 pKa = 4.25LNTPSDD13 pKa = 3.73LATAITRR20 pKa = 11.84CAGHH24 pKa = 5.82VAALVSKK31 pKa = 10.39EE32 pKa = 3.57LDD34 pKa = 3.31ALVRR38 pKa = 11.84TARR41 pKa = 11.84TLQEE45 pKa = 3.91QYY47 pKa = 10.37EE48 pKa = 4.39SRR50 pKa = 11.84RR51 pKa = 11.84TAEE54 pKa = 3.87EE55 pKa = 3.83TRR57 pKa = 11.84WQHH60 pKa = 4.65MVLAIKK66 pKa = 9.03TGPSLVLQWSRR77 pKa = 11.84RR78 pKa = 11.84RR79 pKa = 11.84RR80 pKa = 11.84IRR82 pKa = 11.84RR83 pKa = 11.84TATGRR88 pKa = 11.84AWWWDD93 pKa = 3.31TMPYY97 pKa = 9.53DD98 pKa = 4.83AKK100 pKa = 10.69RR101 pKa = 11.84RR102 pKa = 11.84CCPIRR107 pKa = 11.84ALTANQPPEE116 pKa = 3.64IAALIISTEE125 pKa = 4.02SAATEE130 pKa = 4.0IRR132 pKa = 11.84KK133 pKa = 8.54NWQLVTRR140 pKa = 11.84LRR142 pKa = 11.84ITATALMRR150 pKa = 11.84LGQLAPAEE158 pKa = 4.65SPWLHH163 pKa = 5.42LTGKK167 pKa = 10.1NGSTILAQDD176 pKa = 3.81HH177 pKa = 5.82MQDD180 pKa = 3.47PDD182 pKa = 3.74SFATTVLMFVAALQDD197 pKa = 3.4QLLRR201 pKa = 11.84NGDD204 pKa = 3.81ALSQYY209 pKa = 10.51VEE211 pKa = 4.18NNRR214 pKa = 11.84SNKK217 pKa = 9.58GYY219 pKa = 10.19RR220 pKa = 11.84LRR222 pKa = 11.84SNRR225 pKa = 11.84RR226 pKa = 11.84GNSLDD231 pKa = 3.49LAWYY235 pKa = 8.62HH236 pKa = 6.35GPAASRR242 pKa = 11.84TTVTRR247 pKa = 11.84RR248 pKa = 11.84KK249 pKa = 9.22NANITRR255 pKa = 11.84LIATNSEE262 pKa = 3.97DD263 pKa = 3.34ANLIRR268 pKa = 11.84EE269 pKa = 4.47CEE271 pKa = 3.78AAATVIRR278 pKa = 11.84RR279 pKa = 11.84AWAMCGQLIARR290 pKa = 11.84AKK292 pKa = 10.23EE293 pKa = 3.8IEE295 pKa = 4.41KK296 pKa = 9.12LTKK299 pKa = 10.12ACAPRR304 pKa = 11.84KK305 pKa = 9.56LGEE308 pKa = 3.83IAMGGLGGNPEE319 pKa = 4.21APPRR323 pKa = 11.84SSKK326 pKa = 11.07

Molecular weight: 36.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4694

0

4694

1753483

30

4550

373.6

40.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.987 ± 0.041

0.969 ± 0.011

5.824 ± 0.028

5.114 ± 0.034

3.67 ± 0.02

7.96 ± 0.035

2.122 ± 0.019

4.568 ± 0.022

2.794 ± 0.03

10.668 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.057 ± 0.016

3.2 ± 0.033

5.264 ± 0.033

4.194 ± 0.021

7.005 ± 0.044

6.186 ± 0.037

5.504 ± 0.05

7.128 ± 0.031

1.536 ± 0.018

2.251 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski