Yersinia phage YpP-Y
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6QAD5|I6QAD5_9CAUD Protein inside capsid D OS=Yersinia phage YpP-Y OX=1176766 PE=3 SV=1
MM1 pKa = 7.89 RR2 pKa = 11.84 SYY4 pKa = 11.98 DD5 pKa = 3.77 MNVEE9 pKa = 4.15 TAAEE13 pKa = 4.19 LSAVNDD19 pKa = 3.37 ILASIGEE26 pKa = 4.48 PPVSTLGGDD35 pKa = 3.77 SNADD39 pKa = 3.09 VANARR44 pKa = 11.84 RR45 pKa = 11.84 ILNKK49 pKa = 9.68 INRR52 pKa = 11.84 QIQSRR57 pKa = 11.84 GWTFNIEE64 pKa = 3.91 EE65 pKa = 5.53 GITLLPDD72 pKa = 3.63 VYY74 pKa = 11.35 SNLIVYY80 pKa = 10.33 SDD82 pKa = 4.91 DD83 pKa = 3.71 YY84 pKa = 11.14 LSLMATSGQSIYY96 pKa = 10.6 VNRR99 pKa = 11.84 GGYY102 pKa = 9.88 VYY104 pKa = 10.89 DD105 pKa = 3.76 RR106 pKa = 11.84 TSQSDD111 pKa = 3.41 RR112 pKa = 11.84 FDD114 pKa = 3.28 SGITVNIIRR123 pKa = 11.84 LRR125 pKa = 11.84 DD126 pKa = 3.55 YY127 pKa = 11.62 DD128 pKa = 4.05 EE129 pKa = 4.49 MPEE132 pKa = 4.08 CFRR135 pKa = 11.84 YY136 pKa = 9.62 WIVTKK141 pKa = 10.73 ASRR144 pKa = 11.84 QFNNRR149 pKa = 11.84 FFGAPEE155 pKa = 3.93 VEE157 pKa = 4.4 GVLQEE162 pKa = 4.57 EE163 pKa = 4.18 EE164 pKa = 4.15 DD165 pKa = 3.69 EE166 pKa = 4.24 ARR168 pKa = 11.84 RR169 pKa = 11.84 LCMEE173 pKa = 4.07 YY174 pKa = 10.13 EE175 pKa = 3.93 VDD177 pKa = 3.46 YY178 pKa = 11.41 GGYY181 pKa = 11.22 NMLDD185 pKa = 2.99 GDD187 pKa = 4.62 AFTSGLLTRR196 pKa = 5.06
Molecular weight: 22.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.326
IPC2_protein 4.355
IPC_protein 4.291
Toseland 4.101
ProMoST 4.406
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.139
Rodwell 4.113
Grimsley 4.012
Solomon 4.24
Lehninger 4.202
Nozaki 4.355
DTASelect 4.546
Thurlkill 4.126
EMBOSS 4.151
Sillero 4.393
Patrickios 3.465
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.333
Protein with the highest isoelectric point:
>tr|I6Q986|I6Q986_9CAUD DNA-directed DNA polymerase OS=Yersinia phage YpP-Y OX=1176766 PE=3 SV=1
MM1 pKa = 7.62 RR2 pKa = 11.84 KK3 pKa = 9.46 SYY5 pKa = 10.38 KK6 pKa = 9.86 QFYY9 pKa = 8.52 KK10 pKa = 10.52 APRR13 pKa = 11.84 RR14 pKa = 11.84 HH15 pKa = 4.88 IQVWEE20 pKa = 4.05 AANGPIPKK28 pKa = 9.69 GYY30 pKa = 10.59 YY31 pKa = 8.84 IDD33 pKa = 5.86 HH34 pKa = 7.35 IDD36 pKa = 4.32 GNPLNDD42 pKa = 3.88 ALDD45 pKa = 4.04 NLRR48 pKa = 11.84 LALPKK53 pKa = 10.31 EE54 pKa = 4.42 NSWNMKK60 pKa = 7.49 TPKK63 pKa = 10.51 SNTSGLKK70 pKa = 9.35 GLSWSKK76 pKa = 10.23 EE77 pKa = 3.55 RR78 pKa = 11.84 EE79 pKa = 3.78 MWRR82 pKa = 11.84 GTVTAEE88 pKa = 4.07 GKK90 pKa = 8.14 QHH92 pKa = 5.84 NFRR95 pKa = 11.84 SRR97 pKa = 11.84 DD98 pKa = 3.59 LLEE101 pKa = 4.06 VVAWIYY107 pKa = 8.09 RR108 pKa = 11.84 TRR110 pKa = 11.84 RR111 pKa = 11.84 EE112 pKa = 3.8 LHH114 pKa = 5.41 GQFARR119 pKa = 11.84 FRR121 pKa = 3.88
Molecular weight: 14.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.267
IPC2_protein 9.648
IPC_protein 10.087
Toseland 10.482
ProMoST 10.233
Dawson 10.613
Bjellqvist 10.277
Wikipedia 10.789
Rodwell 10.994
Grimsley 10.672
Solomon 10.672
Lehninger 10.643
Nozaki 10.452
DTASelect 10.277
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.526
Patrickios 10.716
IPC_peptide 10.672
IPC2_peptide 8.873
IPC2.peptide.svr19 8.797
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
10922
37
1318
237.4
26.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.845 ± 0.549
0.998 ± 0.206
6.574 ± 0.261
7.196 ± 0.375
3.653 ± 0.197
7.398 ± 0.344
1.932 ± 0.205
5.301 ± 0.2
6.794 ± 0.464
7.938 ± 0.3
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.957 ± 0.249
4.587 ± 0.385
3.415 ± 0.236
3.818 ± 0.306
5.869 ± 0.248
6.107 ± 0.352
5.503 ± 0.264
6.235 ± 0.234
1.593 ± 0.196
3.287 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here