Bellinger River virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Tornidovirineae; Tobaniviridae; Serpentovirinae; Pregotovirus; Snaturtovirus; Berisnavirus 1

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346I7I9|A0A346I7I9_9NIDO Minor membrane protein 1 OS=Bellinger River virus OX=2301728 GN=MM1 PE=4 SV=1
MM1 pKa = 7.31FLVLLSILATCEE13 pKa = 4.39CQSHH17 pKa = 7.03RR18 pKa = 11.84YY19 pKa = 9.48DD20 pKa = 3.07NWDD23 pKa = 3.58KK24 pKa = 11.58VFTLDD29 pKa = 5.2NNIQNIISQKK39 pKa = 10.0QFDD42 pKa = 4.4NFLEE46 pKa = 4.3QPFLINSISNDD57 pKa = 3.34VKK59 pKa = 8.71PTEE62 pKa = 3.78IVEE65 pKa = 4.29YY66 pKa = 9.33LTNNQLTQALPNKK79 pKa = 9.52LPHH82 pKa = 6.33PVISSVKK89 pKa = 10.15IIDD92 pKa = 4.0KK93 pKa = 8.68QTTACNSQLINCDD106 pKa = 3.43TKK108 pKa = 11.36VKK110 pKa = 9.86TLTNLNGNCFFFNPSSISNRR130 pKa = 11.84SCYY133 pKa = 9.66KK134 pKa = 9.4NKK136 pKa = 9.2IWRR139 pKa = 11.84VRR141 pKa = 11.84NTNDD145 pKa = 4.14NSSTCDD151 pKa = 3.2NVYY154 pKa = 11.12APIGVKK160 pKa = 9.8TITQDD165 pKa = 3.13GKK167 pKa = 10.26QVPLYY172 pKa = 10.07YY173 pKa = 10.15HH174 pKa = 7.73DD175 pKa = 5.36ILPQLYY181 pKa = 9.13EE182 pKa = 3.87PGRR185 pKa = 11.84IVNDD189 pKa = 3.14NRR191 pKa = 11.84EE192 pKa = 4.15CCIFKK197 pKa = 10.0TPSVEE202 pKa = 4.32CKK204 pKa = 10.28SKK206 pKa = 11.07AVLAGGKK213 pKa = 7.1ITNYY217 pKa = 9.71INTFHH222 pKa = 6.36EE223 pKa = 4.75TIIQCPCAQKK233 pKa = 10.7CVIFINSQIDD243 pKa = 3.55VLISDD248 pKa = 5.59GILIKK253 pKa = 10.28STFEE257 pKa = 4.61LEE259 pKa = 3.88NLHH262 pKa = 6.62NIKK265 pKa = 10.26DD266 pKa = 3.64QHH268 pKa = 5.88TKK270 pKa = 10.69SILRR274 pKa = 11.84QQQPEE279 pKa = 4.1TSKK282 pKa = 11.35LEE284 pKa = 3.94RR285 pKa = 11.84QYY287 pKa = 11.83LEE289 pKa = 4.45FNNNCGDD296 pKa = 4.23SITKK300 pKa = 10.12AGCEE304 pKa = 3.84LEE306 pKa = 4.28TIEE309 pKa = 4.8FSSSIYY315 pKa = 9.31MIHH318 pKa = 6.36NAVMPNTIFKK328 pKa = 8.0TFCAVEE334 pKa = 4.09KK335 pKa = 11.02LPINDD340 pKa = 3.37QLEE343 pKa = 4.21PLNSKK348 pKa = 10.2FSVPATVQLGKK359 pKa = 10.12QRR361 pKa = 11.84YY362 pKa = 7.88IAHH365 pKa = 6.95KK366 pKa = 9.06VNPVTFQLDD375 pKa = 3.67YY376 pKa = 11.43TNDD379 pKa = 3.54PDD381 pKa = 5.41FISATTTTLPTIGSCRR397 pKa = 11.84RR398 pKa = 11.84SDD400 pKa = 3.27GYY402 pKa = 11.09AWHH405 pKa = 7.06GNFSIEE411 pKa = 4.08KK412 pKa = 8.38NEE414 pKa = 4.63ARR416 pKa = 11.84IEE418 pKa = 4.32DD419 pKa = 4.19CTATTLDD426 pKa = 3.95WNPVCNITKK435 pKa = 10.42LEE437 pKa = 4.13PLVKK441 pKa = 10.33VYY443 pKa = 10.78AVDD446 pKa = 3.83KK447 pKa = 10.73KK448 pKa = 9.88ITAKK452 pKa = 10.47GVNCDD457 pKa = 4.78DD458 pKa = 3.48ICPYY462 pKa = 11.04AVLCSEE468 pKa = 4.16EE469 pKa = 4.36QKK471 pKa = 10.67RR472 pKa = 11.84SPIYY476 pKa = 9.19QACLAIEE483 pKa = 4.02QDD485 pKa = 2.87ISTLLKK491 pKa = 10.2VEE493 pKa = 4.55RR494 pKa = 11.84PTSVKK499 pKa = 9.53MAALTKK505 pKa = 9.64TEE507 pKa = 4.32PLAGTIDD514 pKa = 3.62VNFLQEE520 pKa = 4.01VAKK523 pKa = 10.85VQAQRR528 pKa = 11.84FDD530 pKa = 3.74LTVEE534 pKa = 3.99TGLDD538 pKa = 3.38HH539 pKa = 6.65TQVLDD544 pKa = 4.11DD545 pKa = 4.35LFKK548 pKa = 10.88LTVALPQDD556 pKa = 3.56STFFPDD562 pKa = 3.61RR563 pKa = 11.84SQAAIDD569 pKa = 4.32LGLFQNAMFNSKK581 pKa = 10.72ADD583 pKa = 3.72MDD585 pKa = 4.31HH586 pKa = 6.34VAAFPWLAGWRR597 pKa = 11.84FGRR600 pKa = 11.84QINGLSYY607 pKa = 8.05TTSNLIRR614 pKa = 11.84SYY616 pKa = 10.77RR617 pKa = 11.84QFSNTVNKK625 pKa = 10.14NFQQVATALSAMADD639 pKa = 3.97QITMNYY645 pKa = 9.49EE646 pKa = 3.65VTTRR650 pKa = 11.84LYY652 pKa = 10.99EE653 pKa = 4.38SIQRR657 pKa = 11.84SYY659 pKa = 10.36QAYY662 pKa = 8.5EE663 pKa = 3.85NNFAKK668 pKa = 9.69IDD670 pKa = 3.65YY671 pKa = 9.74RR672 pKa = 11.84MSEE675 pKa = 4.21IEE677 pKa = 4.97FMTAKK682 pKa = 10.55LSLINQYY689 pKa = 7.41FTKK692 pKa = 10.34IATRR696 pKa = 11.84QSQLASEE703 pKa = 4.39HH704 pKa = 6.18QIFDD708 pKa = 4.03LRR710 pKa = 11.84VGSCRR715 pKa = 11.84TANPACFGGQGVYY728 pKa = 9.73LAHH731 pKa = 7.42AEE733 pKa = 4.26FEE735 pKa = 4.6TPSQKK740 pKa = 10.94LLIVNYY746 pKa = 10.22LGSTEE751 pKa = 3.85KK752 pKa = 10.65CKK754 pKa = 10.49QVFTANKK761 pKa = 8.74ICTKK765 pKa = 10.5QGTATIAPFGCAFFKK780 pKa = 10.78TSDD783 pKa = 3.47SDD785 pKa = 4.62SEE787 pKa = 4.32VLRR790 pKa = 11.84NLKK793 pKa = 10.67DD794 pKa = 3.28NGPCHH799 pKa = 6.52VPPINVQDD807 pKa = 3.97CQLPTNEE814 pKa = 4.83IIQLQLINYY823 pKa = 9.21QEE825 pKa = 4.11TLEE828 pKa = 4.24LGIRR832 pKa = 11.84QVNLTRR838 pKa = 11.84VEE840 pKa = 4.34FNQKK844 pKa = 8.74IANISTFNNNIKK856 pKa = 10.35QLVNSIDD863 pKa = 3.76KK864 pKa = 10.73VPSLGDD870 pKa = 3.35MLEE873 pKa = 4.17KK874 pKa = 9.8QTIASLNSIIAQINAWSIWDD894 pKa = 3.64IIKK897 pKa = 9.99WFLVCGVIIAFSALGISVIKK917 pKa = 10.68LVTKK921 pKa = 10.58RR922 pKa = 3.56

Molecular weight: 103.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346I7J2|A0A346I7J2_9NIDO Minor membrane protein 2 OS=Bellinger River virus OX=2301728 GN=MM2 PE=4 SV=1
MM1 pKa = 7.34ATFLPMPMQLPLMQPTRR18 pKa = 11.84PVQRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84PAKK30 pKa = 9.55RR31 pKa = 11.84QQRR34 pKa = 11.84NPNNAMTKK42 pKa = 9.96KK43 pKa = 10.6VDD45 pKa = 3.56MLTKK49 pKa = 10.58KK50 pKa = 10.25LDD52 pKa = 3.55KK53 pKa = 10.97LMPTVKK59 pKa = 9.91PDD61 pKa = 3.58PNVYY65 pKa = 9.03TFTAKK70 pKa = 10.51YY71 pKa = 9.98GEE73 pKa = 4.31KK74 pKa = 10.16LAPLLITPEE83 pKa = 3.88QDD85 pKa = 2.87SDD87 pKa = 3.71PRR89 pKa = 11.84NHH91 pKa = 6.39MSQEE95 pKa = 3.77AMTKK99 pKa = 10.01FANDD103 pKa = 2.83VSRR106 pKa = 11.84RR107 pKa = 11.84LKK109 pKa = 10.83AGAGTVTIAPTGVVTVHH126 pKa = 6.34LQFKK130 pKa = 10.04PIALNPNAQAFVPRR144 pKa = 11.84DD145 pKa = 3.35LSEE148 pKa = 3.95VSEE151 pKa = 4.31AA152 pKa = 4.14

Molecular weight: 17.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

14867

152

7459

1858.4

209.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.625 ± 0.181

2.556 ± 0.161

4.856 ± 0.317

4.581 ± 0.214

4.365 ± 0.194

3.276 ± 0.146

3.504 ± 0.41

7.305 ± 0.256

5.892 ± 0.214

8.051 ± 0.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.291 ± 0.104

7.533 ± 0.196

3.787 ± 0.223

4.903 ± 0.29

3.538 ± 0.103

7.984 ± 0.328

10.244 ± 0.406

5.139 ± 0.312

0.888 ± 0.1

3.679 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski