Butyrivibrio sp. CAG:318

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Butyrivibrio; environmental samples

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6QU86|R6QU86_9FIRM L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D OS=Butyrivibrio sp. CAG:318 OX=1262761 GN=BN606_01978 PE=4 SV=1
MM1 pKa = 7.15EE2 pKa = 4.9QIFNAFIGMLFLFILTFGGISITTAAIDD30 pKa = 3.61SKK32 pKa = 11.29NAEE35 pKa = 4.2EE36 pKa = 4.51YY37 pKa = 10.39VAEE40 pKa = 4.17AAQIIEE46 pKa = 4.23SSNYY50 pKa = 9.95ADD52 pKa = 5.37DD53 pKa = 4.9VINNLKK59 pKa = 10.62DD60 pKa = 2.9KK61 pKa = 10.76AAASGYY67 pKa = 9.49GFTVNSVDD75 pKa = 4.92LDD77 pKa = 3.8GDD79 pKa = 3.73VAADD83 pKa = 3.31ITEE86 pKa = 3.84VFLDD90 pKa = 4.01HH91 pKa = 7.73KK92 pKa = 8.78YY93 pKa = 10.46QCLLL97 pKa = 3.57

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6RPB6|R6RPB6_9FIRM Argininosuccinate lyase OS=Butyrivibrio sp. CAG:318 OX=1262761 GN=argH PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.02KK10 pKa = 9.86SRR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.67GFRR19 pKa = 11.84KK20 pKa = 9.99RR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.71GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2127

0

2127

697473

29

1623

327.9

36.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.671 ± 0.055

1.48 ± 0.021

6.666 ± 0.049

6.781 ± 0.056

3.888 ± 0.037

7.186 ± 0.051

1.641 ± 0.022

8.265 ± 0.058

6.689 ± 0.042

7.881 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.193 ± 0.028

4.953 ± 0.037

3.068 ± 0.028

2.4 ± 0.025

4.306 ± 0.042

6.025 ± 0.039

5.499 ± 0.046

7.091 ± 0.043

0.782 ± 0.018

4.533 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski