Strawberry latent ringspot virus satellite RNA
Average proteome isoelectric point is 9.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q07996|Q07996_9VIRU 36 kDa protein OS=Strawberry latent ringspot virus satellite RNA OX=195062 PE=4 SV=1
MM1 pKa = 6.67 VQNSVLKK8 pKa = 10.77 GGGGVLPKK16 pKa = 9.96 RR17 pKa = 11.84 VRR19 pKa = 11.84 VISKK23 pKa = 8.91 RR24 pKa = 11.84 RR25 pKa = 11.84 ARR27 pKa = 11.84 IFTVGRR33 pKa = 11.84 HH34 pKa = 5.83 DD35 pKa = 4.17 LHH37 pKa = 6.83 VLPSGKK43 pKa = 9.8 RR44 pKa = 11.84 AWVPVRR50 pKa = 11.84 APIAAGGSFRR60 pKa = 11.84 RR61 pKa = 11.84 EE62 pKa = 3.57 RR63 pKa = 11.84 PPVVSHH69 pKa = 6.34 KK70 pKa = 7.77 TQQVRR75 pKa = 11.84 PIRR78 pKa = 11.84 TPTAVKK84 pKa = 9.38 TSNRR88 pKa = 11.84 FASLSVKK95 pKa = 10.31 EE96 pKa = 4.24 EE97 pKa = 3.9 APEE100 pKa = 3.78 AVAPFNPKK108 pKa = 9.64 KK109 pKa = 10.38 KK110 pKa = 10.27 SYY112 pKa = 11.24 ADD114 pKa = 3.58 ALRR117 pKa = 11.84 SSAFVKK123 pKa = 10.08 PSARR127 pKa = 11.84 KK128 pKa = 9.11 GVVTLVEE135 pKa = 4.26 TEE137 pKa = 3.55 KK138 pKa = 11.28 DD139 pKa = 3.15 FALFCDD145 pKa = 4.5 EE146 pKa = 4.17 FTQNQVVPCPGQTLSEE162 pKa = 4.31 AEE164 pKa = 3.94 SLQRR168 pKa = 11.84 EE169 pKa = 3.89 FHH171 pKa = 5.99 KK172 pKa = 10.8 RR173 pKa = 11.84 AFAFSGKK180 pKa = 9.28 RR181 pKa = 11.84 LSVSGLPEE189 pKa = 4.14 GPIPSLLLRR198 pKa = 11.84 QSACNCPKK206 pKa = 10.14 GCHH209 pKa = 6.03 VDD211 pKa = 3.63 PVSWEE216 pKa = 3.78 GMSFGQCSRR225 pKa = 11.84 ALTLYY230 pKa = 10.34 RR231 pKa = 11.84 QLCGCFGMKK240 pKa = 9.15 MFSSSLYY247 pKa = 9.3 GMAVRR252 pKa = 11.84 RR253 pKa = 11.84 FSALGKK259 pKa = 9.83 ISLWQSRR266 pKa = 11.84 KK267 pKa = 9.25 ILLVTGRR274 pKa = 11.84 LARR277 pKa = 11.84 IWRR280 pKa = 11.84 PSTSLYY286 pKa = 10.68 SYY288 pKa = 8.66 TSSSRR293 pKa = 11.84 FVPMIRR299 pKa = 11.84 SGTWAFFKK307 pKa = 10.69 DD308 pKa = 3.48 SLIIGSTHH316 pKa = 6.17 ACVVHH321 pKa = 6.44 ICSEE325 pKa = 4.3 TGIEE329 pKa = 4.03 PAA331 pKa = 4.45
Molecular weight: 36.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.575
IPC_protein 10.014
Toseland 10.716
ProMoST 10.306
Dawson 10.789
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 11.111
Grimsley 10.818
Solomon 10.877
Lehninger 10.862
Nozaki 10.716
DTASelect 10.438
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.716
Patrickios 10.818
IPC_peptide 10.891
IPC2_peptide 9.589
IPC2.peptide.svr19 8.605
Protein with the highest isoelectric point:
>tr|Q07996|Q07996_9VIRU 36 kDa protein OS=Strawberry latent ringspot virus satellite RNA OX=195062 PE=4 SV=1
MM1 pKa = 6.67 VQNSVLKK8 pKa = 10.77 GGGGVLPKK16 pKa = 9.96 RR17 pKa = 11.84 VRR19 pKa = 11.84 VISKK23 pKa = 8.91 RR24 pKa = 11.84 RR25 pKa = 11.84 ARR27 pKa = 11.84 IFTVGRR33 pKa = 11.84 HH34 pKa = 5.83 DD35 pKa = 4.17 LHH37 pKa = 6.83 VLPSGKK43 pKa = 9.8 RR44 pKa = 11.84 AWVPVRR50 pKa = 11.84 APIAAGGSFRR60 pKa = 11.84 RR61 pKa = 11.84 EE62 pKa = 3.57 RR63 pKa = 11.84 PPVVSHH69 pKa = 6.34 KK70 pKa = 7.77 TQQVRR75 pKa = 11.84 PIRR78 pKa = 11.84 TPTAVKK84 pKa = 9.38 TSNRR88 pKa = 11.84 FASLSVKK95 pKa = 10.31 EE96 pKa = 4.24 EE97 pKa = 3.9 APEE100 pKa = 3.78 AVAPFNPKK108 pKa = 9.64 KK109 pKa = 10.38 KK110 pKa = 10.27 SYY112 pKa = 11.24 ADD114 pKa = 3.58 ALRR117 pKa = 11.84 SSAFVKK123 pKa = 10.08 PSARR127 pKa = 11.84 KK128 pKa = 9.11 GVVTLVEE135 pKa = 4.26 TEE137 pKa = 3.55 KK138 pKa = 11.28 DD139 pKa = 3.15 FALFCDD145 pKa = 4.5 EE146 pKa = 4.17 FTQNQVVPCPGQTLSEE162 pKa = 4.31 AEE164 pKa = 3.94 SLQRR168 pKa = 11.84 EE169 pKa = 3.89 FHH171 pKa = 5.99 KK172 pKa = 10.8 RR173 pKa = 11.84 AFAFSGKK180 pKa = 9.28 RR181 pKa = 11.84 LSVSGLPEE189 pKa = 4.14 GPIPSLLLRR198 pKa = 11.84 QSACNCPKK206 pKa = 10.14 GCHH209 pKa = 6.03 VDD211 pKa = 3.63 PVSWEE216 pKa = 3.78 GMSFGQCSRR225 pKa = 11.84 ALTLYY230 pKa = 10.34 RR231 pKa = 11.84 QLCGCFGMKK240 pKa = 9.15 MFSSSLYY247 pKa = 9.3 GMAVRR252 pKa = 11.84 RR253 pKa = 11.84 FSALGKK259 pKa = 9.83 ISLWQSRR266 pKa = 11.84 KK267 pKa = 9.25 ILLVTGRR274 pKa = 11.84 LARR277 pKa = 11.84 IWRR280 pKa = 11.84 PSTSLYY286 pKa = 10.68 SYY288 pKa = 8.66 TSSSRR293 pKa = 11.84 FVPMIRR299 pKa = 11.84 SGTWAFFKK307 pKa = 10.69 DD308 pKa = 3.48 SLIIGSTHH316 pKa = 6.17 ACVVHH321 pKa = 6.44 ICSEE325 pKa = 4.3 TGIEE329 pKa = 4.03 PAA331 pKa = 4.45
Molecular weight: 36.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.575
IPC_protein 10.014
Toseland 10.716
ProMoST 10.306
Dawson 10.789
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 11.111
Grimsley 10.818
Solomon 10.877
Lehninger 10.862
Nozaki 10.716
DTASelect 10.438
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.716
Patrickios 10.818
IPC_peptide 10.891
IPC2_peptide 9.589
IPC2.peptide.svr19 8.605
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
331
331
331
331.0
36.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.855 ± 0.0
3.021 ± 0.0
1.813 ± 0.0
4.23 ± 0.0
5.438 ± 0.0
7.251 ± 0.0
2.115 ± 0.0
3.927 ± 0.0
6.042 ± 0.0
7.855 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.813 ± 0.0
1.511 ± 0.0
6.647 ± 0.0
3.323 ± 0.0
9.063 ± 0.0
11.48 ± 0.0
4.834 ± 0.0
8.761 ± 0.0
1.511 ± 0.0
1.511 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here