Botrytis cinerea mitovirus 4
Average proteome isoelectric point is 8.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S4GA85|A0A0S4GA85_9VIRU RNA dependent RNA polymerase OS=Botrytis cinerea mitovirus 4 OX=1629667 GN=RdRp PE=4 SV=1
MM1 pKa = 7.42 SNKK4 pKa = 9.58 IINIIIIRR12 pKa = 11.84 IIRR15 pKa = 11.84 LCFNYY20 pKa = 10.38 NEE22 pKa = 4.58 STWEE26 pKa = 3.63 INEE29 pKa = 3.97 FLNIFNNMRR38 pKa = 11.84 KK39 pKa = 9.71 KK40 pKa = 10.69 SGLKK44 pKa = 8.67 YY45 pKa = 8.58 TIKK48 pKa = 10.49 YY49 pKa = 7.87 YY50 pKa = 10.74 KK51 pKa = 10.03 AVKK54 pKa = 9.98 LHH56 pKa = 4.82 ITRR59 pKa = 11.84 YY60 pKa = 9.05 ICGSPLLSNKK70 pKa = 10.39 DD71 pKa = 3.51 GVSVDD76 pKa = 3.21 ASGWPTKK83 pKa = 10.53 FIFLKK88 pKa = 10.71 KK89 pKa = 10.42 FIKK92 pKa = 9.27 TRR94 pKa = 11.84 QGLRR98 pKa = 11.84 ILLTLLSFTRR108 pKa = 11.84 TVVPTKK114 pKa = 10.4 QEE116 pKa = 3.87 EE117 pKa = 4.39 LKK119 pKa = 10.65 IKK121 pKa = 10.05 PDD123 pKa = 3.26 YY124 pKa = 10.33 STIDD128 pKa = 3.11 KK129 pKa = 10.84 LYY131 pKa = 8.7 TGKK134 pKa = 10.51 NYY136 pKa = 8.37 TIPAWFIKK144 pKa = 10.26 SWINKK149 pKa = 8.85 HH150 pKa = 4.87 NLKK153 pKa = 10.74 AKK155 pKa = 9.84 IPTYY159 pKa = 9.91 TKK161 pKa = 8.87 EE162 pKa = 3.73 DD163 pKa = 3.86 HH164 pKa = 5.92 YY165 pKa = 11.88 VSMKK169 pKa = 10.46 GSPNGPATYY178 pKa = 10.43 SSLWSILSLSYY189 pKa = 10.28 PQLQNISTMLGDD201 pKa = 3.77 YY202 pKa = 10.32 RR203 pKa = 11.84 DD204 pKa = 3.95 EE205 pKa = 4.05 FFKK208 pKa = 10.66 FYY210 pKa = 8.96 KK211 pKa = 9.24 TAWEE215 pKa = 4.19 NNFGNDD221 pKa = 3.96 SSIKK225 pKa = 8.29 SSKK228 pKa = 6.63 WTGYY232 pKa = 7.77 TGKK235 pKa = 10.61 LSIVKK240 pKa = 10.24 DD241 pKa = 3.69 PEE243 pKa = 3.88 LKK245 pKa = 10.27 RR246 pKa = 11.84 RR247 pKa = 11.84 VIAMVDD253 pKa = 3.16 YY254 pKa = 10.83 HH255 pKa = 6.82 SQFTLKK261 pKa = 10.17 PIHH264 pKa = 6.83 EE265 pKa = 4.35 MLLNKK270 pKa = 9.89 LRR272 pKa = 11.84 TLKK275 pKa = 10.44 CDD277 pKa = 3.05 RR278 pKa = 11.84 TFTQDD283 pKa = 4.13 PKK285 pKa = 10.98 HH286 pKa = 5.65 SWYY289 pKa = 10.96 VNNEE293 pKa = 3.79 KK294 pKa = 10.47 FFSLDD299 pKa = 3.02 LSAATDD305 pKa = 3.96 RR306 pKa = 11.84 FPLQLQKK313 pKa = 11.35 KK314 pKa = 8.25 LLSYY318 pKa = 10.27 IYY320 pKa = 9.76 EE321 pKa = 4.29 NKK323 pKa = 10.31 DD324 pKa = 3.3 FCDD327 pKa = 3.16 AWADD331 pKa = 3.81 LLTSRR336 pKa = 11.84 VYY338 pKa = 10.45 IDD340 pKa = 4.66 SDD342 pKa = 3.94 GLQHH346 pKa = 7.08 KK347 pKa = 9.93 YY348 pKa = 10.81 NVGQPMGAYY357 pKa = 9.89 SSWAAFTITHH367 pKa = 6.31 HH368 pKa = 7.05 LVVAWAAHH376 pKa = 5.7 LCGEE380 pKa = 4.62 YY381 pKa = 10.72 NFSQYY386 pKa = 10.7 IILGDD391 pKa = 4.59 DD392 pKa = 3.31 IVIKK396 pKa = 10.53 NNKK399 pKa = 7.97 VANKK403 pKa = 9.9 YY404 pKa = 7.31 ITLMTRR410 pKa = 11.84 LGVEE414 pKa = 3.87 ISLHH418 pKa = 5.06 KK419 pKa = 9.43 THH421 pKa = 6.28 VSKK424 pKa = 10.14 DD425 pKa = 3.14 TYY427 pKa = 10.9 EE428 pKa = 3.97 FAKK431 pKa = 10.31 RR432 pKa = 11.84 WIKK435 pKa = 10.86 DD436 pKa = 3.54 GIEE439 pKa = 3.85 VSGIPLKK446 pKa = 11.08 GILNQWKK453 pKa = 9.43 FPGVVYY459 pKa = 7.53 TTLEE463 pKa = 4.01 SFFDD467 pKa = 3.93 KK468 pKa = 11.24 NPIQPKK474 pKa = 10.21 SLIDD478 pKa = 4.57 LICGLYY484 pKa = 10.57 KK485 pKa = 10.53 NLPLGKK491 pKa = 9.52 RR492 pKa = 11.84 RR493 pKa = 11.84 MSYY496 pKa = 9.03 NQIYY500 pKa = 9.96 KK501 pKa = 10.66 LLYY504 pKa = 9.8 DD505 pKa = 3.43 YY506 pKa = 10.54 HH507 pKa = 7.85 HH508 pKa = 7.3 AMRR511 pKa = 11.84 YY512 pKa = 8.49 SLNKK516 pKa = 8.22 ITYY519 pKa = 8.87 DD520 pKa = 3.26 EE521 pKa = 4.26 LRR523 pKa = 11.84 AYY525 pKa = 10.44 LCSKK529 pKa = 10.24 CKK531 pKa = 9.95 EE532 pKa = 4.09 DD533 pKa = 5.1 SFVLPYY539 pKa = 10.74 EE540 pKa = 4.66 SISLHH545 pKa = 5.51 FMKK548 pKa = 10.85 LLLSGGMVSEE558 pKa = 4.35 AEE560 pKa = 4.12 KK561 pKa = 10.51 VSRR564 pKa = 11.84 FILSEE569 pKa = 3.8 YY570 pKa = 10.12 TKK572 pKa = 10.34 IEE574 pKa = 4.08 NKK576 pKa = 10.28 FKK578 pKa = 11.0 DD579 pKa = 3.62 SYY581 pKa = 11.65 SDD583 pKa = 4.09 LNILSGYY590 pKa = 9.03 PLLNGYY596 pKa = 8.75 YY597 pKa = 10.24 NHH599 pKa = 6.59 LQSMQGKK606 pKa = 8.93 ILDD609 pKa = 3.83 WEE611 pKa = 4.23 KK612 pKa = 11.35 DD613 pKa = 3.63 PNVTLVDD620 pKa = 3.62 SALSLRR626 pKa = 11.84 IEE628 pKa = 4.26 KK629 pKa = 9.96 FDD631 pKa = 5.36 KK632 pKa = 10.28 ISSMNRR638 pKa = 11.84 DD639 pKa = 2.71 KK640 pKa = 11.04 SVRR643 pKa = 11.84 VSTLSGLWKK652 pKa = 9.65 TSMKK656 pKa = 10.2 RR657 pKa = 11.84 LWVEE661 pKa = 4.06 RR662 pKa = 11.84 IEE664 pKa = 5.88 DD665 pKa = 3.75 DD666 pKa = 3.74 FEE668 pKa = 5.34 YY669 pKa = 8.29 MTFFSRR675 pKa = 11.84 INKK678 pKa = 8.66 DD679 pKa = 3.01 QHH681 pKa = 8.09 DD682 pKa = 4.3 SMLPVHH688 pKa = 6.14 GWEE691 pKa = 4.4 GVLDD695 pKa = 3.97 NNIQFTLNQLKK706 pKa = 9.83 PLISGTIVKK715 pKa = 9.71 VEE717 pKa = 4.01 KK718 pKa = 10.92 NSWEE722 pKa = 3.96 NLDD725 pKa = 3.43 WGDD728 pKa = 4.03 FKK730 pKa = 11.81 VV731 pKa = 3.4
Molecular weight: 85.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.709
IPC2_protein 8.726
IPC_protein 8.58
Toseland 9.253
ProMoST 9.092
Dawson 9.575
Bjellqvist 9.311
Wikipedia 9.75
Rodwell 9.823
Grimsley 9.663
Solomon 9.589
Lehninger 9.545
Nozaki 9.37
DTASelect 9.268
Thurlkill 9.399
EMBOSS 9.706
Sillero 9.516
Patrickios 4.533
IPC_peptide 9.575
IPC2_peptide 7.849
IPC2.peptide.svr19 7.764
Protein with the highest isoelectric point:
>tr|A0A0S4GA85|A0A0S4GA85_9VIRU RNA dependent RNA polymerase OS=Botrytis cinerea mitovirus 4 OX=1629667 GN=RdRp PE=4 SV=1
MM1 pKa = 7.42 SNKK4 pKa = 9.58 IINIIIIRR12 pKa = 11.84 IIRR15 pKa = 11.84 LCFNYY20 pKa = 10.38 NEE22 pKa = 4.58 STWEE26 pKa = 3.63 INEE29 pKa = 3.97 FLNIFNNMRR38 pKa = 11.84 KK39 pKa = 9.71 KK40 pKa = 10.69 SGLKK44 pKa = 8.67 YY45 pKa = 8.58 TIKK48 pKa = 10.49 YY49 pKa = 7.87 YY50 pKa = 10.74 KK51 pKa = 10.03 AVKK54 pKa = 9.98 LHH56 pKa = 4.82 ITRR59 pKa = 11.84 YY60 pKa = 9.05 ICGSPLLSNKK70 pKa = 10.39 DD71 pKa = 3.51 GVSVDD76 pKa = 3.21 ASGWPTKK83 pKa = 10.53 FIFLKK88 pKa = 10.71 KK89 pKa = 10.42 FIKK92 pKa = 9.27 TRR94 pKa = 11.84 QGLRR98 pKa = 11.84 ILLTLLSFTRR108 pKa = 11.84 TVVPTKK114 pKa = 10.4 QEE116 pKa = 3.87 EE117 pKa = 4.39 LKK119 pKa = 10.65 IKK121 pKa = 10.05 PDD123 pKa = 3.26 YY124 pKa = 10.33 STIDD128 pKa = 3.11 KK129 pKa = 10.84 LYY131 pKa = 8.7 TGKK134 pKa = 10.51 NYY136 pKa = 8.37 TIPAWFIKK144 pKa = 10.26 SWINKK149 pKa = 8.85 HH150 pKa = 4.87 NLKK153 pKa = 10.74 AKK155 pKa = 9.84 IPTYY159 pKa = 9.91 TKK161 pKa = 8.87 EE162 pKa = 3.73 DD163 pKa = 3.86 HH164 pKa = 5.92 YY165 pKa = 11.88 VSMKK169 pKa = 10.46 GSPNGPATYY178 pKa = 10.43 SSLWSILSLSYY189 pKa = 10.28 PQLQNISTMLGDD201 pKa = 3.77 YY202 pKa = 10.32 RR203 pKa = 11.84 DD204 pKa = 3.95 EE205 pKa = 4.05 FFKK208 pKa = 10.66 FYY210 pKa = 8.96 KK211 pKa = 9.24 TAWEE215 pKa = 4.19 NNFGNDD221 pKa = 3.96 SSIKK225 pKa = 8.29 SSKK228 pKa = 6.63 WTGYY232 pKa = 7.77 TGKK235 pKa = 10.61 LSIVKK240 pKa = 10.24 DD241 pKa = 3.69 PEE243 pKa = 3.88 LKK245 pKa = 10.27 RR246 pKa = 11.84 RR247 pKa = 11.84 VIAMVDD253 pKa = 3.16 YY254 pKa = 10.83 HH255 pKa = 6.82 SQFTLKK261 pKa = 10.17 PIHH264 pKa = 6.83 EE265 pKa = 4.35 MLLNKK270 pKa = 9.89 LRR272 pKa = 11.84 TLKK275 pKa = 10.44 CDD277 pKa = 3.05 RR278 pKa = 11.84 TFTQDD283 pKa = 4.13 PKK285 pKa = 10.98 HH286 pKa = 5.65 SWYY289 pKa = 10.96 VNNEE293 pKa = 3.79 KK294 pKa = 10.47 FFSLDD299 pKa = 3.02 LSAATDD305 pKa = 3.96 RR306 pKa = 11.84 FPLQLQKK313 pKa = 11.35 KK314 pKa = 8.25 LLSYY318 pKa = 10.27 IYY320 pKa = 9.76 EE321 pKa = 4.29 NKK323 pKa = 10.31 DD324 pKa = 3.3 FCDD327 pKa = 3.16 AWADD331 pKa = 3.81 LLTSRR336 pKa = 11.84 VYY338 pKa = 10.45 IDD340 pKa = 4.66 SDD342 pKa = 3.94 GLQHH346 pKa = 7.08 KK347 pKa = 9.93 YY348 pKa = 10.81 NVGQPMGAYY357 pKa = 9.89 SSWAAFTITHH367 pKa = 6.31 HH368 pKa = 7.05 LVVAWAAHH376 pKa = 5.7 LCGEE380 pKa = 4.62 YY381 pKa = 10.72 NFSQYY386 pKa = 10.7 IILGDD391 pKa = 4.59 DD392 pKa = 3.31 IVIKK396 pKa = 10.53 NNKK399 pKa = 7.97 VANKK403 pKa = 9.9 YY404 pKa = 7.31 ITLMTRR410 pKa = 11.84 LGVEE414 pKa = 3.87 ISLHH418 pKa = 5.06 KK419 pKa = 9.43 THH421 pKa = 6.28 VSKK424 pKa = 10.14 DD425 pKa = 3.14 TYY427 pKa = 10.9 EE428 pKa = 3.97 FAKK431 pKa = 10.31 RR432 pKa = 11.84 WIKK435 pKa = 10.86 DD436 pKa = 3.54 GIEE439 pKa = 3.85 VSGIPLKK446 pKa = 11.08 GILNQWKK453 pKa = 9.43 FPGVVYY459 pKa = 7.53 TTLEE463 pKa = 4.01 SFFDD467 pKa = 3.93 KK468 pKa = 11.24 NPIQPKK474 pKa = 10.21 SLIDD478 pKa = 4.57 LICGLYY484 pKa = 10.57 KK485 pKa = 10.53 NLPLGKK491 pKa = 9.52 RR492 pKa = 11.84 RR493 pKa = 11.84 MSYY496 pKa = 9.03 NQIYY500 pKa = 9.96 KK501 pKa = 10.66 LLYY504 pKa = 9.8 DD505 pKa = 3.43 YY506 pKa = 10.54 HH507 pKa = 7.85 HH508 pKa = 7.3 AMRR511 pKa = 11.84 YY512 pKa = 8.49 SLNKK516 pKa = 8.22 ITYY519 pKa = 8.87 DD520 pKa = 3.26 EE521 pKa = 4.26 LRR523 pKa = 11.84 AYY525 pKa = 10.44 LCSKK529 pKa = 10.24 CKK531 pKa = 9.95 EE532 pKa = 4.09 DD533 pKa = 5.1 SFVLPYY539 pKa = 10.74 EE540 pKa = 4.66 SISLHH545 pKa = 5.51 FMKK548 pKa = 10.85 LLLSGGMVSEE558 pKa = 4.35 AEE560 pKa = 4.12 KK561 pKa = 10.51 VSRR564 pKa = 11.84 FILSEE569 pKa = 3.8 YY570 pKa = 10.12 TKK572 pKa = 10.34 IEE574 pKa = 4.08 NKK576 pKa = 10.28 FKK578 pKa = 11.0 DD579 pKa = 3.62 SYY581 pKa = 11.65 SDD583 pKa = 4.09 LNILSGYY590 pKa = 9.03 PLLNGYY596 pKa = 8.75 YY597 pKa = 10.24 NHH599 pKa = 6.59 LQSMQGKK606 pKa = 8.93 ILDD609 pKa = 3.83 WEE611 pKa = 4.23 KK612 pKa = 11.35 DD613 pKa = 3.63 PNVTLVDD620 pKa = 3.62 SALSLRR626 pKa = 11.84 IEE628 pKa = 4.26 KK629 pKa = 9.96 FDD631 pKa = 5.36 KK632 pKa = 10.28 ISSMNRR638 pKa = 11.84 DD639 pKa = 2.71 KK640 pKa = 11.04 SVRR643 pKa = 11.84 VSTLSGLWKK652 pKa = 9.65 TSMKK656 pKa = 10.2 RR657 pKa = 11.84 LWVEE661 pKa = 4.06 RR662 pKa = 11.84 IEE664 pKa = 5.88 DD665 pKa = 3.75 DD666 pKa = 3.74 FEE668 pKa = 5.34 YY669 pKa = 8.29 MTFFSRR675 pKa = 11.84 INKK678 pKa = 8.66 DD679 pKa = 3.01 QHH681 pKa = 8.09 DD682 pKa = 4.3 SMLPVHH688 pKa = 6.14 GWEE691 pKa = 4.4 GVLDD695 pKa = 3.97 NNIQFTLNQLKK706 pKa = 9.83 PLISGTIVKK715 pKa = 9.71 VEE717 pKa = 4.01 KK718 pKa = 10.92 NSWEE722 pKa = 3.96 NLDD725 pKa = 3.43 WGDD728 pKa = 4.03 FKK730 pKa = 11.81 VV731 pKa = 3.4
Molecular weight: 85.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.709
IPC2_protein 8.726
IPC_protein 8.58
Toseland 9.253
ProMoST 9.092
Dawson 9.575
Bjellqvist 9.311
Wikipedia 9.75
Rodwell 9.823
Grimsley 9.663
Solomon 9.589
Lehninger 9.545
Nozaki 9.37
DTASelect 9.268
Thurlkill 9.399
EMBOSS 9.706
Sillero 9.516
Patrickios 4.533
IPC_peptide 9.575
IPC2_peptide 7.849
IPC2.peptide.svr19 7.764
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
731
731
731
731.0
85.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.146 ± 0.0
1.094 ± 0.0
5.746 ± 0.0
4.378 ± 0.0
4.651 ± 0.0
4.651 ± 0.0
2.462 ± 0.0
7.934 ± 0.0
9.986 ± 0.0
10.807 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.326 ± 0.0
5.882 ± 0.0
3.283 ± 0.0
2.599 ± 0.0
3.694 ± 0.0
8.755 ± 0.0
5.609 ± 0.0
4.651 ± 0.0
2.599 ± 0.0
5.746 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here