Amino acid dipepetide frequency for Streptomyces phage FlowerPower

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.251AlaAla: 11.251 ± 1.079
0.825AlaCys: 0.825 ± 0.248
4.8AlaAsp: 4.8 ± 0.649
6.526AlaGlu: 6.526 ± 0.652
2.175AlaPhe: 2.175 ± 0.332
8.701AlaGly: 8.701 ± 0.86
1.35AlaHis: 1.35 ± 0.316
3.75AlaIle: 3.75 ± 0.538
6.751AlaLys: 6.751 ± 0.849
7.426AlaLeu: 7.426 ± 0.653
2.325AlaMet: 2.325 ± 0.359
4.2AlaAsn: 4.2 ± 0.58
3.525AlaPro: 3.525 ± 0.691
4.95AlaGln: 4.95 ± 0.604
5.101AlaArg: 5.101 ± 0.749
6.301AlaSer: 6.301 ± 0.71
6.976AlaThr: 6.976 ± 0.962
7.576AlaVal: 7.576 ± 0.751
1.95AlaTrp: 1.95 ± 0.408
3.525AlaTyr: 3.525 ± 0.629
0.0AlaXaa: 0.0 ± 0.0
Cys
0.3CysAla: 0.3 ± 0.136
0.0CysCys: 0.0 ± 0.0
0.525CysAsp: 0.525 ± 0.21
0.45CysGlu: 0.45 ± 0.158
0.075CysPhe: 0.075 ± 0.066
0.525CysGly: 0.525 ± 0.186
0.225CysHis: 0.225 ± 0.129
0.15CysIle: 0.15 ± 0.108
0.6CysLys: 0.6 ± 0.262
0.6CysLeu: 0.6 ± 0.204
0.15CysMet: 0.15 ± 0.115
0.0CysAsn: 0.0 ± 0.0
0.975CysPro: 0.975 ± 0.368
0.3CysGln: 0.3 ± 0.19
0.225CysArg: 0.225 ± 0.119
0.45CysSer: 0.45 ± 0.222
0.3CysThr: 0.3 ± 0.157
0.825CysVal: 0.825 ± 0.268
0.075CysTrp: 0.075 ± 0.073
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
6.451AspAla: 6.451 ± 0.595
0.45AspCys: 0.45 ± 0.161
4.35AspAsp: 4.35 ± 0.587
4.125AspGlu: 4.125 ± 0.584
2.025AspPhe: 2.025 ± 0.345
4.8AspGly: 4.8 ± 0.65
1.275AspHis: 1.275 ± 0.297
3.525AspIle: 3.525 ± 0.505
2.925AspLys: 2.925 ± 0.392
4.275AspLeu: 4.275 ± 0.53
2.625AspMet: 2.625 ± 0.69
2.25AspAsn: 2.25 ± 0.457
4.65AspPro: 4.65 ± 0.488
2.25AspGln: 2.25 ± 0.449
3.225AspArg: 3.225 ± 0.421
3.525AspSer: 3.525 ± 0.523
3.675AspThr: 3.675 ± 0.512
4.875AspVal: 4.875 ± 0.638
1.35AspTrp: 1.35 ± 0.316
2.85AspTyr: 2.85 ± 0.338
0.0AspXaa: 0.0 ± 0.0
Glu
5.626GluAla: 5.626 ± 0.75
0.45GluCys: 0.45 ± 0.224
3.225GluAsp: 3.225 ± 0.455
3.675GluGlu: 3.675 ± 0.498
1.875GluPhe: 1.875 ± 0.277
4.5GluGly: 4.5 ± 0.593
1.125GluHis: 1.125 ± 0.339
3.0GluIle: 3.0 ± 0.488
3.075GluLys: 3.075 ± 0.526
5.551GluLeu: 5.551 ± 0.517
0.975GluMet: 0.975 ± 0.31
2.4GluAsn: 2.4 ± 0.386
1.65GluPro: 1.65 ± 0.365
2.775GluGln: 2.775 ± 0.404
3.0GluArg: 3.0 ± 0.489
2.625GluSer: 2.625 ± 0.324
3.075GluThr: 3.075 ± 0.435
3.825GluVal: 3.825 ± 0.552
1.125GluTrp: 1.125 ± 0.246
2.55GluTyr: 2.55 ± 0.48
0.0GluXaa: 0.0 ± 0.0
Phe
2.4PheAla: 2.4 ± 0.423
0.3PheCys: 0.3 ± 0.155
2.55PheAsp: 2.55 ± 0.525
2.1PheGlu: 2.1 ± 0.435
0.825PhePhe: 0.825 ± 0.243
3.375PheGly: 3.375 ± 0.572
0.525PheHis: 0.525 ± 0.17
1.275PheIle: 1.275 ± 0.382
1.2PheLys: 1.2 ± 0.244
2.025PheLeu: 2.025 ± 0.381
0.9PheMet: 0.9 ± 0.238
1.5PheAsn: 1.5 ± 0.336
1.2PhePro: 1.2 ± 0.355
1.2PheGln: 1.2 ± 0.342
1.5PheArg: 1.5 ± 0.458
2.475PheSer: 2.475 ± 0.358
2.925PheThr: 2.925 ± 0.541
1.5PheVal: 1.5 ± 0.276
0.375PheTrp: 0.375 ± 0.156
1.2PheTyr: 1.2 ± 0.373
0.0PheXaa: 0.0 ± 0.0
Gly
8.251GlyAla: 8.251 ± 0.9
0.6GlyCys: 0.6 ± 0.189
3.975GlyAsp: 3.975 ± 0.52
5.251GlyGlu: 5.251 ± 0.66
2.85GlyPhe: 2.85 ± 0.405
8.626GlyGly: 8.626 ± 0.988
1.725GlyHis: 1.725 ± 0.435
3.75GlyIle: 3.75 ± 0.587
6.001GlyLys: 6.001 ± 0.745
6.751GlyLeu: 6.751 ± 0.735
2.1GlyMet: 2.1 ± 0.325
3.075GlyAsn: 3.075 ± 0.552
2.85GlyPro: 2.85 ± 0.363
3.975GlyGln: 3.975 ± 0.74
3.15GlyArg: 3.15 ± 0.386
5.026GlySer: 5.026 ± 0.429
6.001GlyThr: 6.001 ± 1.138
5.926GlyVal: 5.926 ± 0.672
1.5GlyTrp: 1.5 ± 0.268
3.075GlyTyr: 3.075 ± 0.581
0.0GlyXaa: 0.0 ± 0.0
His
1.2HisAla: 1.2 ± 0.282
0.15HisCys: 0.15 ± 0.112
1.5HisAsp: 1.5 ± 0.37
0.675HisGlu: 0.675 ± 0.235
0.6HisPhe: 0.6 ± 0.203
1.275HisGly: 1.275 ± 0.271
0.375HisHis: 0.375 ± 0.199
0.675HisIle: 0.675 ± 0.214
1.05HisLys: 1.05 ± 0.25
1.2HisLeu: 1.2 ± 0.283
0.375HisMet: 0.375 ± 0.184
0.525HisAsn: 0.525 ± 0.214
0.75HisPro: 0.75 ± 0.227
0.6HisGln: 0.6 ± 0.192
0.3HisArg: 0.3 ± 0.147
1.275HisSer: 1.275 ± 0.281
0.525HisThr: 0.525 ± 0.206
1.65HisVal: 1.65 ± 0.265
0.225HisTrp: 0.225 ± 0.175
0.9HisTyr: 0.9 ± 0.275
0.0HisXaa: 0.0 ± 0.0
Ile
4.65IleAla: 4.65 ± 0.627
0.3IleCys: 0.3 ± 0.187
3.6IleAsp: 3.6 ± 0.591
2.025IleGlu: 2.025 ± 0.367
1.125IlePhe: 1.125 ± 0.274
3.075IleGly: 3.075 ± 0.509
0.9IleHis: 0.9 ± 0.239
2.1IleIle: 2.1 ± 0.4
2.7IleLys: 2.7 ± 0.465
3.225IleLeu: 3.225 ± 0.394
0.975IleMet: 0.975 ± 0.239
1.35IleAsn: 1.35 ± 0.338
1.65IlePro: 1.65 ± 0.403
1.125IleGln: 1.125 ± 0.264
2.85IleArg: 2.85 ± 0.496
2.7IleSer: 2.7 ± 0.49
3.675IleThr: 3.675 ± 0.536
2.175IleVal: 2.175 ± 0.357
0.45IleTrp: 0.45 ± 0.16
1.35IleTyr: 1.35 ± 0.282
0.0IleXaa: 0.0 ± 0.0
Lys
7.051LysAla: 7.051 ± 0.905
0.375LysCys: 0.375 ± 0.181
3.9LysAsp: 3.9 ± 0.494
2.25LysGlu: 2.25 ± 0.324
2.7LysPhe: 2.7 ± 0.474
4.2LysGly: 4.2 ± 0.561
0.975LysHis: 0.975 ± 0.222
2.25LysIle: 2.25 ± 0.368
3.375LysLys: 3.375 ± 0.636
4.35LysLeu: 4.35 ± 0.653
1.725LysMet: 1.725 ± 0.399
2.1LysAsn: 2.1 ± 0.599
2.475LysPro: 2.475 ± 0.553
2.775LysGln: 2.775 ± 0.414
2.7LysArg: 2.7 ± 0.499
2.55LysSer: 2.55 ± 0.416
3.3LysThr: 3.3 ± 0.543
4.875LysVal: 4.875 ± 0.613
1.2LysTrp: 1.2 ± 0.305
2.25LysTyr: 2.25 ± 0.476
0.0LysXaa: 0.0 ± 0.0
Leu
6.376LeuAla: 6.376 ± 0.823
0.3LeuCys: 0.3 ± 0.135
5.176LeuAsp: 5.176 ± 0.695
3.45LeuGlu: 3.45 ± 0.423
3.15LeuPhe: 3.15 ± 0.524
5.476LeuGly: 5.476 ± 0.626
0.6LeuHis: 0.6 ± 0.195
2.85LeuIle: 2.85 ± 0.49
4.725LeuLys: 4.725 ± 0.656
5.026LeuLeu: 5.026 ± 0.432
1.8LeuMet: 1.8 ± 0.389
2.7LeuAsn: 2.7 ± 0.382
3.525LeuPro: 3.525 ± 0.486
3.6LeuGln: 3.6 ± 0.573
4.95LeuArg: 4.95 ± 0.583
4.95LeuSer: 4.95 ± 0.582
6.151LeuThr: 6.151 ± 0.533
5.851LeuVal: 5.851 ± 0.73
0.975LeuTrp: 0.975 ± 0.356
2.625LeuTyr: 2.625 ± 0.398
0.0LeuXaa: 0.0 ± 0.0
Met
4.65MetAla: 4.65 ± 0.539
0.075MetCys: 0.075 ± 0.068
2.025MetAsp: 2.025 ± 0.338
1.2MetGlu: 1.2 ± 0.319
0.525MetPhe: 0.525 ± 0.186
2.775MetGly: 2.775 ± 0.512
0.075MetHis: 0.075 ± 0.074
1.05MetIle: 1.05 ± 0.272
1.05MetLys: 1.05 ± 0.399
1.425MetLeu: 1.425 ± 0.361
0.675MetMet: 0.675 ± 0.233
1.2MetAsn: 1.2 ± 0.253
1.125MetPro: 1.125 ± 0.376
1.35MetGln: 1.35 ± 0.325
1.5MetArg: 1.5 ± 0.303
2.775MetSer: 2.775 ± 0.489
1.425MetThr: 1.425 ± 0.343
1.425MetVal: 1.425 ± 0.288
0.45MetTrp: 0.45 ± 0.177
0.375MetTyr: 0.375 ± 0.157
0.0MetXaa: 0.0 ± 0.0
Asn
3.825AsnAla: 3.825 ± 0.529
0.375AsnCys: 0.375 ± 0.148
2.4AsnAsp: 2.4 ± 0.501
2.7AsnGlu: 2.7 ± 0.468
1.35AsnPhe: 1.35 ± 0.298
4.05AsnGly: 4.05 ± 0.685
0.75AsnHis: 0.75 ± 0.197
1.425AsnIle: 1.425 ± 0.276
2.25AsnLys: 2.25 ± 0.479
3.9AsnLeu: 3.9 ± 0.582
0.45AsnMet: 0.45 ± 0.157
1.875AsnAsn: 1.875 ± 0.471
2.85AsnPro: 2.85 ± 0.532
1.65AsnGln: 1.65 ± 0.33
1.875AsnArg: 1.875 ± 0.409
2.475AsnSer: 2.475 ± 0.352
2.175AsnThr: 2.175 ± 0.308
2.1AsnVal: 2.1 ± 0.421
1.125AsnTrp: 1.125 ± 0.233
1.275AsnTyr: 1.275 ± 0.299
0.0AsnXaa: 0.0 ± 0.0
Pro
4.95ProAla: 4.95 ± 0.763
0.225ProCys: 0.225 ± 0.116
2.4ProAsp: 2.4 ± 0.374
2.625ProGlu: 2.625 ± 0.399
1.35ProPhe: 1.35 ± 0.281
4.125ProGly: 4.125 ± 0.586
0.75ProHis: 0.75 ± 0.202
0.975ProIle: 0.975 ± 0.249
2.1ProLys: 2.1 ± 0.481
3.075ProLeu: 3.075 ± 0.532
1.125ProMet: 1.125 ± 0.288
1.8ProAsn: 1.8 ± 0.305
2.025ProPro: 2.025 ± 0.495
1.8ProGln: 1.8 ± 0.309
2.55ProArg: 2.55 ± 0.403
2.925ProSer: 2.925 ± 0.447
3.75ProThr: 3.75 ± 0.513
3.225ProVal: 3.225 ± 0.428
0.9ProTrp: 0.9 ± 0.256
1.575ProTyr: 1.575 ± 0.368
0.0ProXaa: 0.0 ± 0.0
Gln
6.076GlnAla: 6.076 ± 0.857
0.15GlnCys: 0.15 ± 0.099
3.3GlnAsp: 3.3 ± 0.384
2.4GlnGlu: 2.4 ± 0.507
0.825GlnPhe: 0.825 ± 0.261
2.325GlnGly: 2.325 ± 0.485
0.225GlnHis: 0.225 ± 0.139
2.1GlnIle: 2.1 ± 0.498
2.475GlnLys: 2.475 ± 0.471
3.9GlnLeu: 3.9 ± 0.577
1.575GlnMet: 1.575 ± 0.322
1.125GlnAsn: 1.125 ± 0.214
1.5GlnPro: 1.5 ± 0.365
2.4GlnGln: 2.4 ± 0.492
2.85GlnArg: 2.85 ± 0.403
3.3GlnSer: 3.3 ± 0.528
3.075GlnThr: 3.075 ± 0.456
3.0GlnVal: 3.0 ± 0.429
0.3GlnTrp: 0.3 ± 0.138
1.65GlnTyr: 1.65 ± 0.336
0.0GlnXaa: 0.0 ± 0.0
Arg
4.425ArgAla: 4.425 ± 0.604
0.525ArgCys: 0.525 ± 0.225
3.9ArgAsp: 3.9 ± 0.444
2.925ArgGlu: 2.925 ± 0.472
2.025ArgPhe: 2.025 ± 0.381
3.75ArgGly: 3.75 ± 0.394
0.825ArgHis: 0.825 ± 0.21
2.175ArgIle: 2.175 ± 0.356
2.55ArgLys: 2.55 ± 0.432
3.0ArgLeu: 3.0 ± 0.466
1.425ArgMet: 1.425 ± 0.315
2.4ArgAsn: 2.4 ± 0.465
2.025ArgPro: 2.025 ± 0.333
1.875ArgGln: 1.875 ± 0.433
2.175ArgArg: 2.175 ± 0.609
2.85ArgSer: 2.85 ± 0.533
3.525ArgThr: 3.525 ± 0.471
4.125ArgVal: 4.125 ± 0.607
1.2ArgTrp: 1.2 ± 0.268
1.8ArgTyr: 1.8 ± 0.364
0.0ArgXaa: 0.0 ± 0.0
Ser
5.251SerAla: 5.251 ± 0.594
0.525SerCys: 0.525 ± 0.205
4.125SerAsp: 4.125 ± 0.589
2.925SerGlu: 2.925 ± 0.48
2.475SerPhe: 2.475 ± 0.372
7.876SerGly: 7.876 ± 0.909
0.9SerHis: 0.9 ± 0.232
3.0SerIle: 3.0 ± 0.381
1.875SerLys: 1.875 ± 0.314
4.2SerLeu: 4.2 ± 0.53
1.725SerMet: 1.725 ± 0.435
3.375SerAsn: 3.375 ± 0.442
2.925SerPro: 2.925 ± 0.466
2.85SerGln: 2.85 ± 0.383
2.625SerArg: 2.625 ± 0.4
5.176SerSer: 5.176 ± 0.797
4.425SerThr: 4.425 ± 0.752
4.35SerVal: 4.35 ± 0.683
1.2SerTrp: 1.2 ± 0.329
2.475SerTyr: 2.475 ± 0.451
0.0SerXaa: 0.0 ± 0.0
Thr
6.151ThrAla: 6.151 ± 0.981
0.375ThrCys: 0.375 ± 0.16
4.425ThrAsp: 4.425 ± 0.575
2.85ThrGlu: 2.85 ± 0.451
2.325ThrPhe: 2.325 ± 0.331
6.226ThrGly: 6.226 ± 0.819
1.275ThrHis: 1.275 ± 0.321
3.075ThrIle: 3.075 ± 0.659
3.975ThrLys: 3.975 ± 0.582
5.626ThrLeu: 5.626 ± 0.9
1.875ThrMet: 1.875 ± 0.449
3.15ThrAsn: 3.15 ± 0.601
4.125ThrPro: 4.125 ± 0.475
2.85ThrGln: 2.85 ± 0.513
2.625ThrArg: 2.625 ± 0.517
4.8ThrSer: 4.8 ± 0.653
5.701ThrThr: 5.701 ± 0.671
4.5ThrVal: 4.5 ± 0.699
1.05ThrTrp: 1.05 ± 0.223
3.15ThrTyr: 3.15 ± 0.356
0.0ThrXaa: 0.0 ± 0.0
Val
6.301ValAla: 6.301 ± 0.92
0.375ValCys: 0.375 ± 0.18
5.026ValAsp: 5.026 ± 0.65
4.35ValGlu: 4.35 ± 0.869
2.025ValPhe: 2.025 ± 0.388
4.425ValGly: 4.425 ± 0.513
1.5ValHis: 1.5 ± 0.327
2.925ValIle: 2.925 ± 0.334
4.8ValLys: 4.8 ± 0.6
4.875ValLeu: 4.875 ± 0.442
2.1ValMet: 2.1 ± 0.268
3.375ValAsn: 3.375 ± 0.61
2.475ValPro: 2.475 ± 0.478
3.3ValGln: 3.3 ± 0.482
3.9ValArg: 3.9 ± 0.507
4.8ValSer: 4.8 ± 0.466
5.851ValThr: 5.851 ± 0.857
3.525ValVal: 3.525 ± 0.779
1.05ValTrp: 1.05 ± 0.28
3.075ValTyr: 3.075 ± 0.532
0.0ValXaa: 0.0 ± 0.0
Trp
1.575TrpAla: 1.575 ± 0.366
0.075TrpCys: 0.075 ± 0.073
1.35TrpAsp: 1.35 ± 0.342
1.05TrpGlu: 1.05 ± 0.261
0.45TrpPhe: 0.45 ± 0.182
0.9TrpGly: 0.9 ± 0.211
0.15TrpHis: 0.15 ± 0.116
0.75TrpIle: 0.75 ± 0.229
1.125TrpLys: 1.125 ± 0.258
1.05TrpLeu: 1.05 ± 0.254
0.45TrpMet: 0.45 ± 0.16
0.75TrpAsn: 0.75 ± 0.238
0.675TrpPro: 0.675 ± 0.255
0.975TrpGln: 0.975 ± 0.279
0.825TrpArg: 0.825 ± 0.201
0.9TrpSer: 0.9 ± 0.279
1.425TrpThr: 1.425 ± 0.288
1.8TrpVal: 1.8 ± 0.276
0.45TrpTrp: 0.45 ± 0.174
0.675TrpTyr: 0.675 ± 0.241
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.225TyrAla: 3.225 ± 0.563
0.375TyrCys: 0.375 ± 0.171
2.925TyrAsp: 2.925 ± 0.427
2.55TyrGlu: 2.55 ± 0.536
0.6TyrPhe: 0.6 ± 0.187
3.45TyrGly: 3.45 ± 0.485
0.3TyrHis: 0.3 ± 0.154
1.2TyrIle: 1.2 ± 0.272
2.775TyrLys: 2.775 ± 0.449
2.775TyrLeu: 2.775 ± 0.379
1.725TyrMet: 1.725 ± 0.341
1.725TyrAsn: 1.725 ± 0.458
1.275TyrPro: 1.275 ± 0.317
1.95TyrGln: 1.95 ± 0.306
1.575TyrArg: 1.575 ± 0.357
2.4TyrSer: 2.4 ± 0.339
2.175TyrThr: 2.175 ± 0.34
2.925TyrVal: 2.925 ± 0.528
0.45TyrTrp: 0.45 ± 0.209
1.8TyrTyr: 1.8 ± 0.378
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (13333 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski