Enterococcus phage vB_EfaS_Ef6.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; unclassified Efquatrovirus

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DSE9|A0A4D6DSE9_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaS_Ef6.1 OX=2546631 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.27EE3 pKa = 4.09SVFSKK8 pKa = 10.68KK9 pKa = 10.37VVDD12 pKa = 3.61YY13 pKa = 11.35LEE15 pKa = 4.81AKK17 pKa = 9.66GAVVNVNTATIYY29 pKa = 10.95DD30 pKa = 3.67RR31 pKa = 11.84VGRR34 pKa = 11.84ADD36 pKa = 4.15IEE38 pKa = 4.28ACYY41 pKa = 10.01SGYY44 pKa = 10.96YY45 pKa = 9.08IALEE49 pKa = 4.29LKK51 pKa = 9.0TGNYY55 pKa = 9.45QPDD58 pKa = 3.98PLQIRR63 pKa = 11.84YY64 pKa = 7.94LQQVRR69 pKa = 11.84CAGGYY74 pKa = 10.4GLLLRR79 pKa = 11.84DD80 pKa = 4.16TLGDD84 pKa = 4.08LYY86 pKa = 11.5DD87 pKa = 4.05LLQYY91 pKa = 11.07LDD93 pKa = 3.59QLDD96 pKa = 3.49NRR98 pKa = 11.84EE99 pKa = 4.08YY100 pKa = 10.9QGFEE104 pKa = 4.31DD105 pKa = 4.26IYY107 pKa = 10.09YY108 pKa = 9.81TYY110 pKa = 9.78EE111 pKa = 4.13QPDD114 pKa = 4.07LPDD117 pKa = 3.26ITDD120 pKa = 3.88DD121 pKa = 3.85KK122 pKa = 11.98VEE124 pKa = 4.04VLYY127 pKa = 10.86DD128 pKa = 3.36

Molecular weight: 14.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DS23|A0A4D6DS23_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaS_Ef6.1 OX=2546631 PE=4 SV=1
MM1 pKa = 7.61IKK3 pKa = 10.2LLKK6 pKa = 10.52AIAFALLVTLGIALPLAFVFIIYY29 pKa = 9.35MSYY32 pKa = 8.44VTAGWAGIWLMLIVLYY48 pKa = 10.66IMIKK52 pKa = 10.25

Molecular weight: 5.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

12327

43

1410

186.8

21.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.857 ± 0.399

0.681 ± 0.141

6.23 ± 0.246

8.283 ± 0.574

4.21 ± 0.224

6.514 ± 0.54

1.533 ± 0.166

6.847 ± 0.234

8.899 ± 0.364

8.348 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.856 ± 0.165

6.157 ± 0.286

2.88 ± 0.239

3.813 ± 0.208

3.407 ± 0.236

5.054 ± 0.274

6.182 ± 0.331

6.717 ± 0.208

1.314 ± 0.095

4.218 ± 0.353
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski