Pseudomonas phage Zuri
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C1K5H7|A0A5C1K5H7_9CAUD Uncharacterized protein OS=Pseudomonas phage Zuri OX=2604899 GN=Zuri_35 PE=4 SV=1
MM1 pKa = 7.42 NNYY4 pKa = 9.17 PDD6 pKa = 4.57 EE7 pKa = 4.97 FEE9 pKa = 5.35 LPADD13 pKa = 3.91 HH14 pKa = 7.94 DD15 pKa = 3.46 IHH17 pKa = 6.27 EE18 pKa = 4.76 LLRR21 pKa = 11.84 ITEE24 pKa = 4.45 TICDD28 pKa = 3.67 EE29 pKa = 4.26 MEE31 pKa = 4.15 TPNGSGSSS39 pKa = 3.46
Molecular weight: 4.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.801
IPC2_protein 3.986
IPC_protein 3.808
Toseland 3.656
ProMoST 3.986
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.719
Rodwell 3.668
Grimsley 3.579
Solomon 3.757
Lehninger 3.719
Nozaki 3.935
DTASelect 4.05
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.935
Patrickios 1.875
IPC_peptide 3.757
IPC2_peptide 3.91
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0A5C1K6U0|A0A5C1K6U0_9CAUD Shock protein A OS=Pseudomonas phage Zuri OX=2604899 GN=Zuri_3 PE=4 SV=1
MM1 pKa = 7.83 AIICRR6 pKa = 11.84 TTKK9 pKa = 10.46 QDD11 pKa = 3.75 INTCKK16 pKa = 10.29 CSRR19 pKa = 11.84 CHH21 pKa = 5.39 PHH23 pKa = 6.19 QEE25 pKa = 3.43 ARR27 pKa = 11.84 YY28 pKa = 8.6 GHH30 pKa = 5.91 VQRR33 pKa = 11.84 QVASRR38 pKa = 11.84 QAGSKK43 pKa = 10.24 KK44 pKa = 10.19 LVLVRR49 pKa = 11.84 VV50 pKa = 3.87
Molecular weight: 5.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 9.399
IPC_protein 9.633
Toseland 10.438
ProMoST 9.999
Dawson 10.54
Bjellqvist 10.218
Wikipedia 10.672
Rodwell 10.935
Grimsley 10.57
Solomon 10.613
Lehninger 10.599
Nozaki 10.511
DTASelect 10.175
Thurlkill 10.438
EMBOSS 10.833
Sillero 10.482
Patrickios 10.774
IPC_peptide 10.628
IPC2_peptide 9.604
IPC2.peptide.svr19 8.258
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
99
0
99
23976
29
3474
242.2
26.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.418 ± 0.479
0.859 ± 0.133
5.935 ± 0.166
6.582 ± 0.281
3.545 ± 0.179
6.648 ± 0.23
2.01 ± 0.203
5.335 ± 0.264
5.973 ± 0.299
8.821 ± 0.212
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.853 ± 0.144
4.872 ± 0.182
4.379 ± 0.145
5.222 ± 0.304
4.817 ± 0.206
5.568 ± 0.238
5.889 ± 0.28
6.84 ± 0.192
1.293 ± 0.131
3.116 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here