Kitasatospora setae (strain ATCC 33774 / DSM 43861 / JCM 3304 / KCC A-0304 / NBRC 14216 / KM-6054) (Streptomyces setae)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Kitasatospora; Kitasatospora setae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7443 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4N2T3|E4N2T3_KITSK Robl_LC7 domain-containing protein OS=Kitasatospora setae (strain ATCC 33774 / DSM 43861 / JCM 3304 / KCC A-0304 / NBRC 14216 / KM-6054) OX=452652 GN=KSE_67090 PE=4 SV=1
MM1 pKa = 7.74AGTGEE6 pKa = 4.21ALEE9 pKa = 4.12VWIDD13 pKa = 3.41QDD15 pKa = 3.99LCTGDD20 pKa = 5.01GICAQYY26 pKa = 10.9APEE29 pKa = 4.23VFEE32 pKa = 5.9LDD34 pKa = 3.05IDD36 pKa = 3.65GLAYY40 pKa = 10.69VKK42 pKa = 11.17GEE44 pKa = 4.07DD45 pKa = 4.75DD46 pKa = 4.81EE47 pKa = 6.03LRR49 pKa = 11.84QQPGQSVPVPLTLLQDD65 pKa = 3.97VVDD68 pKa = 4.33SAKK71 pKa = 10.65DD72 pKa = 3.57CPGDD76 pKa = 4.13CIHH79 pKa = 6.47VRR81 pKa = 11.84RR82 pKa = 11.84AADD85 pKa = 3.45GVEE88 pKa = 4.48VYY90 pKa = 10.92GPDD93 pKa = 3.7AEE95 pKa = 4.31

Molecular weight: 10.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4NAR0|E4NAR0_KITSK Uncharacterized protein OS=Kitasatospora setae (strain ATCC 33774 / DSM 43861 / JCM 3304 / KCC A-0304 / NBRC 14216 / KM-6054) OX=452652 GN=KSE_24770 PE=4 SV=1
MM1 pKa = 7.17PQALRR6 pKa = 11.84VRR8 pKa = 11.84RR9 pKa = 11.84VLKK12 pKa = 10.51ARR14 pKa = 11.84QVPKK18 pKa = 10.38AQQALPVLRR27 pKa = 11.84VRR29 pKa = 11.84LATQVLRR36 pKa = 11.84ARR38 pKa = 11.84QALMAPPAPKK48 pKa = 10.09ARR50 pKa = 11.84RR51 pKa = 11.84ARR53 pKa = 11.84QALRR57 pKa = 11.84ARR59 pKa = 11.84RR60 pKa = 11.84VLRR63 pKa = 11.84ARR65 pKa = 11.84PALMVPPVPKK75 pKa = 10.38ARR77 pKa = 11.84QGPLARR83 pKa = 11.84RR84 pKa = 11.84ARR86 pKa = 11.84QALRR90 pKa = 11.84VRR92 pKa = 11.84SARR95 pKa = 11.84QALLVPKK102 pKa = 10.33ARR104 pKa = 11.84RR105 pKa = 11.84ALQAQPVLMAPPVPKK120 pKa = 10.34ARR122 pKa = 11.84QVRR125 pKa = 11.84LATQVPLAPKK135 pKa = 9.98ARR137 pKa = 11.84QALRR141 pKa = 11.84ARR143 pKa = 11.84PAPSALPAPKK153 pKa = 10.05AQRR156 pKa = 11.84ALMARR161 pKa = 11.84QVRR164 pKa = 11.84RR165 pKa = 11.84VRR167 pKa = 11.84PVLRR171 pKa = 11.84VRR173 pKa = 11.84PVLLVLKK180 pKa = 10.1DD181 pKa = 3.87RR182 pKa = 11.84PAILALPVPKK192 pKa = 10.23ARR194 pKa = 11.84RR195 pKa = 11.84VFKK198 pKa = 10.65ALRR201 pKa = 11.84ARR203 pKa = 11.84RR204 pKa = 11.84APKK207 pKa = 9.98DD208 pKa = 3.19QRR210 pKa = 11.84ALMARR215 pKa = 11.84QVRR218 pKa = 11.84RR219 pKa = 11.84ALPALKK225 pKa = 10.2DD226 pKa = 3.9RR227 pKa = 11.84PATPALPAPKK237 pKa = 9.93ARR239 pKa = 11.84RR240 pKa = 11.84ALQARR245 pKa = 11.84QARR248 pKa = 11.84QVRR251 pKa = 11.84RR252 pKa = 11.84VRR254 pKa = 11.84PATRR258 pKa = 11.84VPPAPKK264 pKa = 9.92ARR266 pKa = 11.84QVRR269 pKa = 11.84LATPAPKK276 pKa = 10.05DD277 pKa = 3.42QRR279 pKa = 11.84ALQAQSARR287 pKa = 11.84RR288 pKa = 11.84APRR291 pKa = 11.84ARR293 pKa = 11.84PAPSALPAPKK303 pKa = 10.0ARR305 pKa = 11.84RR306 pKa = 11.84ALQAQPGRR314 pKa = 11.84QVRR317 pKa = 11.84RR318 pKa = 11.84VRR320 pKa = 11.84PATRR324 pKa = 11.84VPPAPKK330 pKa = 10.0ARR332 pKa = 11.84QVQLATPAPKK342 pKa = 10.12DD343 pKa = 3.27QRR345 pKa = 11.84EE346 pKa = 4.06PLARR350 pKa = 11.84QARR353 pKa = 11.84QVRR356 pKa = 11.84RR357 pKa = 11.84VRR359 pKa = 11.84PATRR363 pKa = 11.84VPPAPKK369 pKa = 9.92ARR371 pKa = 11.84QVRR374 pKa = 11.84LATPAPKK381 pKa = 10.14DD382 pKa = 3.47RR383 pKa = 11.84PARR386 pKa = 11.84RR387 pKa = 11.84APRR390 pKa = 11.84ARR392 pKa = 11.84RR393 pKa = 11.84ALRR396 pKa = 11.84ARR398 pKa = 11.84PARR401 pKa = 11.84QVRR404 pKa = 11.84PATRR408 pKa = 11.84VPPAPKK414 pKa = 9.92ARR416 pKa = 11.84QVRR419 pKa = 11.84LATPAPKK426 pKa = 10.09DD427 pKa = 3.27QRR429 pKa = 11.84EE430 pKa = 4.06PLARR434 pKa = 11.84QARR437 pKa = 11.84QVRR440 pKa = 11.84QAPKK444 pKa = 9.57AQQALRR450 pKa = 11.84ARR452 pKa = 11.84PARR455 pKa = 11.84RR456 pKa = 11.84VLPALRR462 pKa = 11.84GRR464 pKa = 11.84PATQVRR470 pKa = 11.84RR471 pKa = 11.84VLRR474 pKa = 11.84ARR476 pKa = 11.84SALPVLKK483 pKa = 10.44DD484 pKa = 3.91RR485 pKa = 11.84PATPALPAPKK495 pKa = 9.75DD496 pKa = 3.63RR497 pKa = 11.84RR498 pKa = 11.84VLRR501 pKa = 11.84AQQARR506 pKa = 11.84RR507 pKa = 11.84APRR510 pKa = 11.84AQPAPSALPALPAPKK525 pKa = 9.81DD526 pKa = 3.49RR527 pKa = 11.84PARR530 pKa = 11.84RR531 pKa = 11.84VLPALRR537 pKa = 11.84GRR539 pKa = 11.84PATQVRR545 pKa = 11.84RR546 pKa = 11.84VLRR549 pKa = 11.84ARR551 pKa = 11.84SALPVLKK558 pKa = 10.44DD559 pKa = 3.91RR560 pKa = 11.84PATPALPAPKK570 pKa = 9.84DD571 pKa = 3.78RR572 pKa = 11.84QVLRR576 pKa = 11.84ARR578 pKa = 11.84QARR581 pKa = 11.84RR582 pKa = 11.84APRR585 pKa = 11.84GRR587 pKa = 11.84PAPSVLLARR596 pKa = 11.84RR597 pKa = 11.84VPAARR602 pKa = 11.84PVLLARR608 pKa = 11.84RR609 pKa = 11.84VRR611 pKa = 11.84PGRR614 pKa = 11.84RR615 pKa = 11.84VPLAPPVPGGRR626 pKa = 11.84PGRR629 pKa = 11.84PGRR632 pKa = 11.84PGCRR636 pKa = 11.84II637 pKa = 3.33

Molecular weight: 70.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7443

0

7443

2504147

29

6512

336.4

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.001 ± 0.054

0.762 ± 0.007

5.785 ± 0.022

5.324 ± 0.033

2.544 ± 0.014

9.748 ± 0.031

2.298 ± 0.015

2.59 ± 0.02

1.705 ± 0.023

11.03 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.435 ± 0.013

1.662 ± 0.019

6.767 ± 0.031

2.716 ± 0.018

8.279 ± 0.036

4.692 ± 0.024

6.022 ± 0.038

8.124 ± 0.025

1.528 ± 0.012

1.985 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski