Thermoplasmatales archaeon (strain BRNA1)
Average proteome isoelectric point is 5.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1528 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M4YM78|M4YM78_THEXX Delta-aminolevulinic acid dehydratase OS=Thermoplasmatales archaeon (strain BRNA1) OX=1054217 GN=TALC_01208 PE=3 SV=1
MM1 pKa = 7.73 EE2 pKa = 5.48 LRR4 pKa = 11.84 NGEE7 pKa = 3.68 IGLIVLLAGIPVIIAGFLCFTEE29 pKa = 4.4 IVGLGLAALFYY40 pKa = 10.91 VGVAMIIIGAALCYY54 pKa = 9.83 IDD56 pKa = 5.24 VKK58 pKa = 11.17 GSDD61 pKa = 4.03 GVFEE65 pKa = 4.96 QYY67 pKa = 11.29 DD68 pKa = 3.83 SLCALRR74 pKa = 11.84 KK75 pKa = 9.46 MPDD78 pKa = 3.64 DD79 pKa = 5.5 DD80 pKa = 5.48 YY81 pKa = 11.37 MIDD84 pKa = 3.64 LTGSEE89 pKa = 4.11 QKK91 pKa = 10.73 YY92 pKa = 10.38 DD93 pKa = 3.79 LPEE96 pKa = 4.04 VTDD99 pKa = 3.55 MTKK102 pKa = 10.73 EE103 pKa = 4.1 GGQQ106 pKa = 3.24
Molecular weight: 11.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.948
IPC_protein 3.872
Toseland 3.668
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.795
Nozaki 3.986
DTASelect 4.19
Thurlkill 3.732
EMBOSS 3.808
Sillero 3.999
Patrickios 0.846
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.884
Protein with the highest isoelectric point:
>tr|M4YJZ0|M4YJZ0_THEXX Mevalonate kinase OS=Thermoplasmatales archaeon (strain BRNA1) OX=1054217 GN=TALC_00436 PE=3 SV=1
MM1 pKa = 6.86 TVKK4 pKa = 10.29 SEE6 pKa = 4.0 RR7 pKa = 11.84 GRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 YY12 pKa = 9.69 CAFRR16 pKa = 11.84 TDD18 pKa = 3.38 PSTTRR23 pKa = 11.84 EE24 pKa = 3.91 TLIPRR29 pKa = 11.84 LPKK32 pKa = 9.62 DD33 pKa = 3.17 HH34 pKa = 7.04 RR35 pKa = 11.84 FKK37 pKa = 11.1 VIQCAGGMAAIRR49 pKa = 11.84 CSPEE53 pKa = 4.15 DD54 pKa = 3.61 VEE56 pKa = 4.47 EE57 pKa = 4.37 CAAAMKK63 pKa = 10.47 RR64 pKa = 11.84 ADD66 pKa = 4.0 PSSEE70 pKa = 3.83 MVRR73 pKa = 11.84 VSGTIRR79 pKa = 11.84 TLRR82 pKa = 11.84 DD83 pKa = 3.06 RR84 pKa = 11.84 YY85 pKa = 10.69 SVLRR89 pKa = 11.84 ANAPPRR95 pKa = 11.84 PPRR98 pKa = 11.84 KK99 pKa = 8.28 GTPPVAVRR107 pKa = 11.84 EE108 pKa = 4.28 ARR110 pKa = 11.84 KK111 pKa = 9.31 KK112 pKa = 10.85 APSDD116 pKa = 3.53 DD117 pKa = 4.49 SSIKK121 pKa = 10.06 PP122 pKa = 3.48
Molecular weight: 13.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.487
IPC_protein 10.145
Toseland 10.716
ProMoST 10.379
Dawson 10.789
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 10.979
Grimsley 10.818
Solomon 10.921
Lehninger 10.891
Nozaki 10.716
DTASelect 10.482
Thurlkill 10.701
EMBOSS 11.125
Sillero 10.73
Patrickios 10.716
IPC_peptide 10.935
IPC2_peptide 9.663
IPC2.peptide.svr19 8.647
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1528
0
1528
443297
14
2782
290.1
32.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.467 ± 0.077
1.665 ± 0.028
6.789 ± 0.052
6.717 ± 0.08
3.862 ± 0.043
8.051 ± 0.07
1.626 ± 0.027
6.811 ± 0.058
5.689 ± 0.073
7.956 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.406 ± 0.048
3.46 ± 0.046
4.014 ± 0.037
2.316 ± 0.028
5.362 ± 0.072
6.287 ± 0.071
5.325 ± 0.073
7.853 ± 0.06
0.919 ± 0.02
3.422 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here