Clostridium sp. CAG:413

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2197 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6PI16|R6PI16_9CLOT Anaerobic ribonucleoside-triphosphate reductase activating protein OS=Clostridium sp. CAG:413 OX=1262803 GN=BN649_01382 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.31NYY4 pKa = 10.31DD5 pKa = 4.05SNASDD10 pKa = 3.75PTADD14 pKa = 3.47LYY16 pKa = 11.58VYY18 pKa = 10.67EE19 pKa = 5.19DD20 pKa = 3.51GKK22 pKa = 10.95EE23 pKa = 3.9KK24 pKa = 10.49QVASNVSTDD33 pKa = 4.27SLICSQDD40 pKa = 2.79GVLGYY45 pKa = 10.29ISVDD49 pKa = 3.24DD50 pKa = 3.75SGARR54 pKa = 11.84SFGIYY59 pKa = 10.34GGGEE63 pKa = 3.92AVTASSGVSEE73 pKa = 4.34ILLLVV78 pKa = 3.75

Molecular weight: 8.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6NZE0|R6NZE0_9CLOT RNA polymerase sigma-24 subunit ECF subfamily protein OS=Clostridium sp. CAG:413 OX=1262803 GN=BN649_02193 PE=3 SV=1
MM1 pKa = 6.26QTASFASVAQLVEE14 pKa = 3.97QWTEE18 pKa = 3.63NPRR21 pKa = 11.84VAGSTPAGGTNFADD35 pKa = 3.63VAHH38 pKa = 6.21LVEE41 pKa = 4.07RR42 pKa = 11.84HH43 pKa = 4.57LAKK46 pKa = 10.76VEE48 pKa = 4.11VASSSLVIRR57 pKa = 11.84SIFLVSFLFTAHH69 pKa = 6.23NLIVRR74 pKa = 11.84CHH76 pKa = 5.32SQVVRR81 pKa = 11.84QRR83 pKa = 11.84SAKK86 pKa = 9.83PRR88 pKa = 11.84FPSSNLGGTSRR99 pKa = 11.84VKK101 pKa = 10.63APKK104 pKa = 9.87SLYY107 pKa = 9.55IKK109 pKa = 10.72AFGVFLLPEE118 pKa = 4.15RR119 pKa = 11.84NFAAVKK125 pKa = 10.23QNSKK129 pKa = 10.35LAPKK133 pKa = 10.15LPNFCRR139 pKa = 11.84ILGCFANFAEE149 pKa = 4.96LFLHH153 pKa = 5.41TTSNVKK159 pKa = 10.21RR160 pKa = 11.84CIHH163 pKa = 6.65YY164 pKa = 10.55IPFLCIRR171 pKa = 11.84QVSFYY176 pKa = 10.89FSLFLQKK183 pKa = 10.34RR184 pKa = 11.84RR185 pKa = 3.67

Molecular weight: 20.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2197

0

2197

709010

30

2860

322.7

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.907 ± 0.061

1.8 ± 0.027

6.101 ± 0.042

6.838 ± 0.055

4.307 ± 0.034

7.56 ± 0.042

1.575 ± 0.019

6.869 ± 0.04

6.352 ± 0.043

8.571 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.029

4.096 ± 0.038

3.638 ± 0.026

2.282 ± 0.027

4.727 ± 0.048

6.394 ± 0.047

5.437 ± 0.048

7.108 ± 0.038

0.865 ± 0.021

3.956 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski