Amino acid dipepetide frequency for Clostridium sp. CAG:413

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.145AlaAla: 11.145 ± 0.189	1.425AlaCys: 1.425 ± 0.045	5.863AlaAsp: 5.863 ± 0.097	7.006AlaGlu: 7.006 ± 0.126	3.639AlaPhe: 3.639 ± 0.093	6.159AlaGly: 6.159 ± 0.1	1.307AlaHis: 1.307 ± 0.051	4.989AlaIle: 4.989 ± 0.08	5.381AlaLys: 5.381 ± 0.1	8.268AlaLeu: 8.268 ± 0.138	2.268AlaMet: 2.268 ± 0.064	2.894AlaAsn: 2.894 ± 0.074	2.977AlaPro: 2.977 ± 0.076	2.41AlaGln: 2.41 ± 0.058	3.503AlaArg: 3.503 ± 0.071	4.588AlaSer: 4.588 ± 0.086	3.575AlaThr: 3.575 ± 0.087	8.073AlaVal: 8.073 ± 0.135	0.583AlaTrp: 0.583 ± 0.03	3.013AlaTyr: 3.013 ± 0.07	0.006AlaXaa: 0.006 ± 0.003
Cys	1.611CysAla: 1.611 ± 0.055	0.391CysCys: 0.391 ± 0.027	1.219CysAsp: 1.219 ± 0.042	1.154CysGlu: 1.154 ± 0.045	0.704CysPhe: 0.704 ± 0.034	2.137CysGly: 2.137 ± 0.077	0.327CysHis: 0.327 ± 0.02	1.014CysIle: 1.014 ± 0.038	0.982CysLys: 0.982 ± 0.042	1.252CysLeu: 1.252 ± 0.048	0.347CysMet: 0.347 ± 0.022	0.616CysAsn: 0.616 ± 0.032	0.814CysPro: 0.814 ± 0.044	0.262CysGln: 0.262 ± 0.02	0.982CysArg: 0.982 ± 0.043	1.309CysSer: 1.309 ± 0.044	0.901CysThr: 0.901 ± 0.044	1.291CysVal: 1.291 ± 0.042	0.128CysTrp: 0.128 ± 0.014	0.56CysTyr: 0.56 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.478AspAla: 4.478 ± 0.089	1.127AspCys: 1.127 ± 0.05	3.468AspAsp: 3.468 ± 0.086	4.908AspGlu: 4.908 ± 0.09	2.994AspPhe: 2.994 ± 0.063	5.918AspGly: 5.918 ± 0.109	0.856AspHis: 0.856 ± 0.037	5.247AspIle: 5.247 ± 0.088	4.185AspLys: 4.185 ± 0.087	4.097AspLeu: 4.097 ± 0.076	1.722AspMet: 1.722 ± 0.05	2.382AspAsn: 2.382 ± 0.057	2.319AspPro: 2.319 ± 0.066	0.771AspGln: 0.771 ± 0.037	2.824AspArg: 2.824 ± 0.064	3.752AspSer: 3.752 ± 0.077	2.963AspThr: 2.963 ± 0.068	3.58AspVal: 3.58 ± 0.068	0.605AspTrp: 0.605 ± 0.031	2.81AspTyr: 2.81 ± 0.065	0.003AspXaa: 0.003 ± 0.002
Glu	5.525GluAla: 5.525 ± 0.098	1.08GluCys: 1.08 ± 0.048	3.365GluAsp: 3.365 ± 0.068	4.495GluGlu: 4.495 ± 0.099	3.027GluPhe: 3.027 ± 0.075	4.1GluGly: 4.1 ± 0.079	1.257GluHis: 1.257 ± 0.048	5.097GluIle: 5.097 ± 0.093	5.228GluLys: 5.228 ± 0.092	6.664GluLeu: 6.664 ± 0.136	2.015GluMet: 2.015 ± 0.051	3.467GluAsn: 3.467 ± 0.077	2.155GluPro: 2.155 ± 0.055	2.267GluGln: 2.267 ± 0.068	3.938GluArg: 3.938 ± 0.087	3.691GluSer: 3.691 ± 0.079	3.516GluThr: 3.516 ± 0.081	3.788GluVal: 3.788 ± 0.071	0.638GluTrp: 0.638 ± 0.031	3.066GluTyr: 3.066 ± 0.069	0.0GluXaa: 0.0 ± 0.0
Phe	4.186PheAla: 4.186 ± 0.082	0.859PheCys: 0.859 ± 0.034	3.172PheAsp: 3.172 ± 0.072	2.836PheGlu: 2.836 ± 0.068	1.865PhePhe: 1.865 ± 0.055	3.57PheGly: 3.57 ± 0.076	0.564PheHis: 0.564 ± 0.033	2.921PheIle: 2.921 ± 0.076	2.416PheLys: 2.416 ± 0.061	3.121PheLeu: 3.121 ± 0.066	1.066PheMet: 1.066 ± 0.04	1.78PheAsn: 1.78 ± 0.055	1.378PhePro: 1.378 ± 0.042	0.628PheGln: 0.628 ± 0.031	1.779PheArg: 1.779 ± 0.05	3.137PheSer: 3.137 ± 0.079	2.763PheThr: 2.763 ± 0.073	2.965PheVal: 2.965 ± 0.079	0.371PheTrp: 0.371 ± 0.027	1.69PheTyr: 1.69 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	5.907GlyAla: 5.907 ± 0.105	1.54GlyCys: 1.54 ± 0.057	4.374GlyAsp: 4.374 ± 0.094	5.526GlyGlu: 5.526 ± 0.101	3.505GlyPhe: 3.505 ± 0.076	6.035GlyGly: 6.035 ± 0.107	1.165GlyHis: 1.165 ± 0.047	5.434GlyIle: 5.434 ± 0.104	5.663GlyLys: 5.663 ± 0.097	5.646GlyLeu: 5.646 ± 0.096	2.154GlyMet: 2.154 ± 0.059	2.911GlyAsn: 2.911 ± 0.079	1.08GlyPro: 1.08 ± 0.044	1.553GlyGln: 1.553 ± 0.049	3.669GlyArg: 3.669 ± 0.079	5.107GlySer: 5.107 ± 0.086	4.152GlyThr: 4.152 ± 0.097	6.021GlyVal: 6.021 ± 0.093	0.822GlyTrp: 0.822 ± 0.054	3.33GlyTyr: 3.33 ± 0.081	0.003GlyXaa: 0.003 ± 0.002
His	1.079HisAla: 1.079 ± 0.037	0.367HisCys: 0.367 ± 0.024	0.942HisAsp: 0.942 ± 0.04	0.932HisGlu: 0.932 ± 0.044	0.759HisPhe: 0.759 ± 0.033	1.367HisGly: 1.367 ± 0.043	0.32HisHis: 0.32 ± 0.024	1.227HisIle: 1.227 ± 0.047	0.797HisLys: 0.797 ± 0.036	1.221HisLeu: 1.221 ± 0.045	0.423HisMet: 0.423 ± 0.026	0.729HisAsn: 0.729 ± 0.031	0.759HisPro: 0.759 ± 0.035	0.341HisGln: 0.341 ± 0.021	0.767HisArg: 0.767 ± 0.035	1.127HisSer: 1.127 ± 0.04	0.922HisThr: 0.922 ± 0.044	0.805HisVal: 0.805 ± 0.039	0.183HisTrp: 0.183 ± 0.017	0.681HisTyr: 0.681 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	6.248IleAla: 6.248 ± 0.102	1.344IleCys: 1.344 ± 0.039	4.451IleAsp: 4.451 ± 0.078	4.536IleGlu: 4.536 ± 0.077	2.812IlePhe: 2.812 ± 0.068	4.918IleGly: 4.918 ± 0.097	0.989IleHis: 0.989 ± 0.037	4.983IleIle: 4.983 ± 0.1	4.479IleLys: 4.479 ± 0.089	5.135IleLeu: 5.135 ± 0.107	1.643IleMet: 1.643 ± 0.056	3.032IleAsn: 3.032 ± 0.07	2.848IlePro: 2.848 ± 0.065	1.214IleGln: 1.214 ± 0.04	3.096IleArg: 3.096 ± 0.08	4.928IleSer: 4.928 ± 0.09	4.113IleThr: 4.113 ± 0.087	4.912IleVal: 4.912 ± 0.1	0.505IleTrp: 0.505 ± 0.027	2.505IleTyr: 2.505 ± 0.069	0.0IleXaa: 0.0 ± 0.0
Lys	5.644LysAla: 5.644 ± 0.079	0.882LysCys: 0.882 ± 0.038	3.408LysAsp: 3.408 ± 0.07	4.392LysGlu: 4.392 ± 0.091	2.145LysPhe: 2.145 ± 0.048	4.111LysGly: 4.111 ± 0.07	0.92LysHis: 0.92 ± 0.034	4.251LysIle: 4.251 ± 0.083	4.984LysLys: 4.984 ± 0.097	5.633LysLeu: 5.633 ± 0.097	1.973LysMet: 1.973 ± 0.049	3.272LysAsn: 3.272 ± 0.066	2.375LysPro: 2.375 ± 0.075	1.65LysGln: 1.65 ± 0.046	3.042LysArg: 3.042 ± 0.07	3.527LysSer: 3.527 ± 0.073	3.783LysThr: 3.783 ± 0.08	4.038LysVal: 4.038 ± 0.085	0.609LysTrp: 0.609 ± 0.032	2.881LysTyr: 2.881 ± 0.058	0.001LysXaa: 0.001 ± 0.002
Leu	7.606LeuAla: 7.606 ± 0.111	1.884LeuCys: 1.884 ± 0.057	5.322LeuAsp: 5.322 ± 0.094	5.086LeuGlu: 5.086 ± 0.095	3.587LeuPhe: 3.587 ± 0.081	6.412LeuGly: 6.412 ± 0.108	1.388LeuHis: 1.388 ± 0.045	5.767LeuIle: 5.767 ± 0.112	4.986LeuLys: 4.986 ± 0.085	7.824LeuLeu: 7.824 ± 0.146	2.199LeuMet: 2.199 ± 0.058	3.402LeuAsn: 3.402 ± 0.067	3.874LeuPro: 3.874 ± 0.076	1.616LeuGln: 1.616 ± 0.047	4.52LeuArg: 4.52 ± 0.089	6.183LeuSer: 6.183 ± 0.107	5.278LeuThr: 5.278 ± 0.085	5.133LeuVal: 5.133 ± 0.085	0.715LeuTrp: 0.715 ± 0.033	2.927LeuTyr: 2.927 ± 0.073	0.001LeuXaa: 0.001 ± 0.001
Met	2.113MetAla: 2.113 ± 0.064	0.388MetCys: 0.388 ± 0.022	1.255MetAsp: 1.255 ± 0.045	1.315MetGlu: 1.315 ± 0.041	0.955MetPhe: 0.955 ± 0.039	1.704MetGly: 1.704 ± 0.052	0.434MetHis: 0.434 ± 0.024	1.728MetIle: 1.728 ± 0.048	2.062MetLys: 2.062 ± 0.05	2.891MetLeu: 2.891 ± 0.076	0.749MetMet: 0.749 ± 0.033	1.238MetAsn: 1.238 ± 0.046	1.337MetPro: 1.337 ± 0.05	0.865MetGln: 0.865 ± 0.034	1.379MetArg: 1.379 ± 0.041	1.705MetSer: 1.705 ± 0.048	1.63MetThr: 1.63 ± 0.051	1.41MetVal: 1.41 ± 0.051	0.196MetTrp: 0.196 ± 0.016	0.769MetTyr: 0.769 ± 0.036	0.001MetXaa: 0.001 ± 0.001
Asn	3.65AsnAla: 3.65 ± 0.074	0.79AsnCys: 0.79 ± 0.036	2.489AsnAsp: 2.489 ± 0.068	2.676AsnGlu: 2.676 ± 0.067	1.606AsnPhe: 1.606 ± 0.05	3.91AsnGly: 3.91 ± 0.095	0.619AsnHis: 0.619 ± 0.028	3.272AsnIle: 3.272 ± 0.077	2.292AsnLys: 2.292 ± 0.064	2.915AsnLeu: 2.915 ± 0.073	1.063AsnMet: 1.063 ± 0.034	1.639AsnAsn: 1.639 ± 0.052	1.805AsnPro: 1.805 ± 0.053	0.793AsnGln: 0.793 ± 0.034	1.676AsnArg: 1.676 ± 0.045	2.735AsnSer: 2.735 ± 0.072	2.164AsnThr: 2.164 ± 0.062	2.818AsnVal: 2.818 ± 0.064	0.35AsnTrp: 0.35 ± 0.023	1.693AsnTyr: 1.693 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	3.417ProAla: 3.417 ± 0.097	0.601ProCys: 0.601 ± 0.032	2.74ProAsp: 2.74 ± 0.067	3.489ProGlu: 3.489 ± 0.072	1.587ProPhe: 1.587 ± 0.053	2.111ProGly: 2.111 ± 0.063	0.654ProHis: 0.654 ± 0.033	2.023ProIle: 2.023 ± 0.052	1.98ProLys: 1.98 ± 0.056	3.041ProLeu: 3.041 ± 0.07	0.88ProMet: 0.88 ± 0.037	1.303ProAsn: 1.303 ± 0.042	1.039ProPro: 1.039 ± 0.039	1.149ProGln: 1.149 ± 0.037	1.298ProArg: 1.298 ± 0.041	2.23ProSer: 2.23 ± 0.066	1.975ProThr: 1.975 ± 0.063	3.07ProVal: 3.07 ± 0.07	0.343ProTrp: 0.343 ± 0.026	1.444ProTyr: 1.444 ± 0.046	0.003ProXaa: 0.003 ± 0.002
Gln	1.889GlnAla: 1.889 ± 0.051	0.322GlnCys: 0.322 ± 0.023	0.987GlnAsp: 0.987 ± 0.041	1.281GlnGlu: 1.281 ± 0.044	0.886GlnPhe: 0.886 ± 0.034	1.417GlnGly: 1.417 ± 0.046	0.396GlnHis: 0.396 ± 0.024	1.643GlnIle: 1.643 ± 0.051	1.687GlnLys: 1.687 ± 0.054	2.236GlnLeu: 2.236 ± 0.052	0.653GlnMet: 0.653 ± 0.03	1.075GlnAsn: 1.075 ± 0.034	0.865GlnPro: 0.865 ± 0.032	0.787GlnGln: 0.787 ± 0.047	1.371GlnArg: 1.371 ± 0.051	1.451GlnSer: 1.451 ± 0.049	1.371GlnThr: 1.371 ± 0.048	1.35GlnVal: 1.35 ± 0.046	0.22GlnTrp: 0.22 ± 0.019	0.934GlnTyr: 0.934 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	3.544ArgAla: 3.544 ± 0.065	0.803ArgCys: 0.803 ± 0.039	2.542ArgAsp: 2.542 ± 0.064	3.598ArgGlu: 3.598 ± 0.078	2.142ArgPhe: 2.142 ± 0.049	2.776ArgGly: 2.776 ± 0.075	0.803ArgHis: 0.803 ± 0.039	3.629ArgIle: 3.629 ± 0.068	2.798ArgLys: 2.798 ± 0.075	4.87ArgLeu: 4.87 ± 0.094	1.261ArgMet: 1.261 ± 0.047	1.997ArgAsn: 1.997 ± 0.058	1.535ArgPro: 1.535 ± 0.046	1.258ArgGln: 1.258 ± 0.042	3.061ArgArg: 3.061 ± 0.082	3.085ArgSer: 3.085 ± 0.067	2.234ArgThr: 2.234 ± 0.053	3.07ArgVal: 3.07 ± 0.075	0.379ArgTrp: 0.379 ± 0.025	1.877ArgTyr: 1.877 ± 0.054	0.004ArgXaa: 0.004 ± 0.002
Ser	6.038SerAla: 6.038 ± 0.101	1.063SerCys: 1.063 ± 0.04	4.092SerAsp: 4.092 ± 0.08	4.21SerGlu: 4.21 ± 0.088	3.083SerPhe: 3.083 ± 0.074	5.865SerGly: 5.865 ± 0.096	1.031SerHis: 1.031 ± 0.038	3.743SerIle: 3.743 ± 0.083	3.487SerLys: 3.487 ± 0.076	5.75SerLeu: 5.75 ± 0.09	1.504SerMet: 1.504 ± 0.053	2.161SerAsn: 2.161 ± 0.067	2.204SerPro: 2.204 ± 0.058	1.472SerGln: 1.472 ± 0.045	2.89SerArg: 2.89 ± 0.073	4.082SerSer: 4.082 ± 0.1	2.886SerThr: 2.886 ± 0.06	5.417SerVal: 5.417 ± 0.094	0.52SerTrp: 0.52 ± 0.03	2.434SerTyr: 2.434 ± 0.066	0.003SerXaa: 0.003 ± 0.002
Thr	5.694ThrAla: 5.694 ± 0.107	0.687ThrCys: 0.687 ± 0.041	3.685ThrAsp: 3.685 ± 0.078	3.629ThrGlu: 3.629 ± 0.075	2.196ThrPhe: 2.196 ± 0.07	4.485ThrGly: 4.485 ± 0.093	0.903ThrHis: 0.903 ± 0.042	2.883ThrIle: 2.883 ± 0.074	2.667ThrLys: 2.667 ± 0.065	4.9ThrLeu: 4.9 ± 0.086	1.236ThrMet: 1.236 ± 0.051	1.748ThrAsn: 1.748 ± 0.049	2.43ThrPro: 2.43 ± 0.063	1.226ThrGln: 1.226 ± 0.041	1.897ThrArg: 1.897 ± 0.048	2.838ThrSer: 2.838 ± 0.061	2.787ThrThr: 2.787 ± 0.11	6.0ThrVal: 6.0 ± 0.129	0.415ThrTrp: 0.415 ± 0.031	2.061ThrTyr: 2.061 ± 0.069	0.001ThrXaa: 0.001 ± 0.001
Val	5.623ValAla: 5.623 ± 0.101	1.599ValCys: 1.599 ± 0.05	4.183ValAsp: 4.183 ± 0.073	4.348ValGlu: 4.348 ± 0.077	3.319ValPhe: 3.319 ± 0.066	4.766ValGly: 4.766 ± 0.08	0.952ValHis: 0.952 ± 0.042	5.453ValIle: 5.453 ± 0.107	4.735ValLys: 4.735 ± 0.087	6.2ValLeu: 6.2 ± 0.109	1.821ValMet: 1.821 ± 0.054	3.013ValAsn: 3.013 ± 0.069	2.929ValPro: 2.929 ± 0.072	1.361ValGln: 1.361 ± 0.041	3.329ValArg: 3.329 ± 0.069	5.082ValSer: 5.082 ± 0.092	4.659ValThr: 4.659 ± 0.119	4.509ValVal: 4.509 ± 0.098	0.619ValTrp: 0.619 ± 0.028	2.577ValTyr: 2.577 ± 0.075	0.003ValXaa: 0.003 ± 0.002
Trp	0.606TrpAla: 0.606 ± 0.031	0.158TrpCys: 0.158 ± 0.018	0.566TrpAsp: 0.566 ± 0.03	0.522TrpGlu: 0.522 ± 0.032	0.413TrpPhe: 0.413 ± 0.026	0.725TrpGly: 0.725 ± 0.035	0.185TrpHis: 0.185 ± 0.02	0.52TrpIle: 0.52 ± 0.028	0.451TrpLys: 0.451 ± 0.029	0.876TrpLeu: 0.876 ± 0.036	0.22TrpMet: 0.22 ± 0.018	0.46TrpAsn: 0.46 ± 0.027	0.141TrpPro: 0.141 ± 0.015	0.386TrpGln: 0.386 ± 0.024	0.437TrpArg: 0.437 ± 0.023	0.587TrpSer: 0.587 ± 0.032	0.386TrpThr: 0.386 ± 0.028	0.516TrpVal: 0.516 ± 0.027	0.099TrpTrp: 0.099 ± 0.012	0.399TrpTyr: 0.399 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.061TyrAla: 3.061 ± 0.068	0.69TyrCys: 0.69 ± 0.038	2.886TyrAsp: 2.886 ± 0.066	2.44TyrGlu: 2.44 ± 0.066	1.839TyrPhe: 1.839 ± 0.051	3.106TyrGly: 3.106 ± 0.064	0.68TyrHis: 0.68 ± 0.034	2.867TyrIle: 2.867 ± 0.064	2.264TyrLys: 2.264 ± 0.053	3.166TyrLeu: 3.166 ± 0.07	0.927TyrMet: 0.927 ± 0.037	1.836TyrAsn: 1.836 ± 0.058	1.512TyrPro: 1.512 ± 0.048	0.808TyrGln: 0.808 ± 0.035	1.81TyrArg: 1.81 ± 0.056	2.79TyrSer: 2.79 ± 0.067	2.306TyrThr: 2.306 ± 0.084	2.309TyrVal: 2.309 ± 0.062	0.354TyrTrp: 0.354 ± 0.02	1.911TyrTyr: 1.911 ± 0.063	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.007XaaAla: 0.007 ± 0.003	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.003XaaPhe: 0.003 ± 0.002	0.006XaaGly: 0.006 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.003XaaSer: 0.003 ± 0.002	0.001XaaThr: 0.001 ± 0.001	0.003XaaVal: 0.003 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.03XaaXaa: 0.03 ± 0.007

Statistics based on 2197 proteins (709010 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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