candidate division MSBL1 archaeon SCGC-AAA261O19
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 779 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A133VEJ6|A0A133VEJ6_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA261O19 OX=1698277 GN=AKJ48_01075 PE=4 SV=1
MM1 pKa = 7.71 PNVIYY6 pKa = 10.26 DD7 pKa = 3.61 YY8 pKa = 11.37 GKK10 pKa = 10.58 CNGNASCAEE19 pKa = 4.0 VCPVNILEE27 pKa = 4.15 EE28 pKa = 4.49 SEE30 pKa = 3.73 NGRR33 pKa = 11.84 WCKK36 pKa = 10.44 PIDD39 pKa = 3.4 EE40 pKa = 4.37 KK41 pKa = 11.71 VEE43 pKa = 3.81 NEE45 pKa = 3.9 EE46 pKa = 4.12 AVKK49 pKa = 10.41 QFHH52 pKa = 6.6 EE53 pKa = 4.48 EE54 pKa = 4.11 VEE56 pKa = 4.24 EE57 pKa = 5.14 SEE59 pKa = 4.43 TQLDD63 pKa = 3.79 VTIEE67 pKa = 3.95 NDD69 pKa = 3.31 MPEE72 pKa = 5.31 CIQCMACVASCPEE85 pKa = 3.87 DD86 pKa = 4.59 AIQIEE91 pKa = 4.38
Molecular weight: 10.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.9
IPC2_protein 3.973
IPC_protein 3.846
Toseland 3.694
ProMoST 3.923
Dawson 3.795
Bjellqvist 3.999
Wikipedia 3.656
Rodwell 3.694
Grimsley 3.617
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 3.986
Thurlkill 3.719
EMBOSS 3.668
Sillero 3.961
Patrickios 0.248
IPC_peptide 3.783
IPC2_peptide 3.948
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|A0A133VD18|A0A133VD18_9EURY Ornithine carbamoyltransferase OS=candidate division MSBL1 archaeon SCGC-AAA261O19 OX=1698277 GN=AKJ48_02975 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.43 SVGGTPRR9 pKa = 11.84 FYY11 pKa = 10.28 PKK13 pKa = 10.52 EE14 pKa = 4.47 GITLRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 GSWAWTEE28 pKa = 3.84 MLFDD32 pKa = 5.57 LMVDD36 pKa = 3.59 PQRR39 pKa = 11.84 WLRR42 pKa = 11.84 EE43 pKa = 3.56 YY44 pKa = 10.3 HH45 pKa = 5.65 VRR47 pKa = 11.84 SNVEE51 pKa = 3.79 SGFSIFTRR59 pKa = 11.84 DD60 pKa = 3.18 FLAPLRR66 pKa = 11.84 KK67 pKa = 9.18 RR68 pKa = 11.84 IHH70 pKa = 6.32 RR71 pKa = 11.84 RR72 pKa = 11.84 RR73 pKa = 11.84 KK74 pKa = 7.27 TEE76 pKa = 3.61 AFARR80 pKa = 11.84 TCDD83 pKa = 3.73 YY84 pKa = 11.17 NLKK87 pKa = 7.93 QACYY91 pKa = 10.14 ARR93 pKa = 11.84 HH94 pKa = 5.27 QEE96 pKa = 4.06 GLIAPWMNTT105 pKa = 2.93
Molecular weight: 12.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.677
IPC_protein 10.584
Toseland 10.57
ProMoST 10.335
Dawson 10.701
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 10.833
Grimsley 10.774
Solomon 10.818
Lehninger 10.789
Nozaki 10.57
DTASelect 10.438
Thurlkill 10.599
EMBOSS 10.979
Sillero 10.643
Patrickios 10.57
IPC_peptide 10.818
IPC2_peptide 9.575
IPC2.peptide.svr19 8.494
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
779
0
779
190618
50
1136
244.7
27.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.876 ± 0.093
0.965 ± 0.036
5.485 ± 0.081
9.471 ± 0.113
3.708 ± 0.061
7.659 ± 0.086
1.829 ± 0.038
7.209 ± 0.079
7.179 ± 0.113
9.752 ± 0.086
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.216 ± 0.035
3.346 ± 0.06
4.264 ± 0.054
2.459 ± 0.042
5.716 ± 0.083
6.025 ± 0.08
4.934 ± 0.058
6.974 ± 0.071
1.114 ± 0.036
2.817 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here