candidate division MSBL1 archaeon SCGC-AAA261O19 
Average proteome isoelectric point is 6.33 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 779 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A133VEJ6|A0A133VEJ6_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA261O19 OX=1698277 GN=AKJ48_01075 PE=4 SV=1MM1 pKa = 7.71  PNVIYY6 pKa = 10.26  DD7 pKa = 3.61  YY8 pKa = 11.37  GKK10 pKa = 10.58  CNGNASCAEE19 pKa = 4.0  VCPVNILEE27 pKa = 4.15  EE28 pKa = 4.49  SEE30 pKa = 3.73  NGRR33 pKa = 11.84  WCKK36 pKa = 10.44  PIDD39 pKa = 3.4  EE40 pKa = 4.37  KK41 pKa = 11.71  VEE43 pKa = 3.81  NEE45 pKa = 3.9  EE46 pKa = 4.12  AVKK49 pKa = 10.41  QFHH52 pKa = 6.6  EE53 pKa = 4.48  EE54 pKa = 4.11  VEE56 pKa = 4.24  EE57 pKa = 5.14  SEE59 pKa = 4.43  TQLDD63 pKa = 3.79  VTIEE67 pKa = 3.95  NDD69 pKa = 3.31  MPEE72 pKa = 5.31  CIQCMACVASCPEE85 pKa = 3.87  DD86 pKa = 4.59  AIQIEE91 pKa = 4.38  
 10.23 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.9 
IPC2_protein 3.973 
IPC_protein 3.846 
Toseland    3.694 
ProMoST     3.923 
Dawson      3.795 
Bjellqvist  3.999 
Wikipedia   3.656 
Rodwell     3.694 
Grimsley    3.617 
Solomon     3.77 
Lehninger   3.732 
Nozaki      3.91 
DTASelect   3.986 
Thurlkill   3.719 
EMBOSS      3.668 
Sillero     3.961 
Patrickios  0.248 
IPC_peptide 3.783 
IPC2_peptide  3.948 
IPC2.peptide.svr19  3.901 
 Protein with the highest isoelectric point: 
>tr|A0A133VD18|A0A133VD18_9EURY Ornithine carbamoyltransferase OS=candidate division MSBL1 archaeon SCGC-AAA261O19 OX=1698277 GN=AKJ48_02975 PE=3 SV=1MM1 pKa = 7.44  KK2 pKa = 10.43  SVGGTPRR9 pKa = 11.84  FYY11 pKa = 10.28  PKK13 pKa = 10.52  EE14 pKa = 4.47  GITLRR19 pKa = 11.84  RR20 pKa = 11.84  RR21 pKa = 11.84  GSWAWTEE28 pKa = 3.84  MLFDD32 pKa = 5.57  LMVDD36 pKa = 3.59  PQRR39 pKa = 11.84  WLRR42 pKa = 11.84  EE43 pKa = 3.56  YY44 pKa = 10.3  HH45 pKa = 5.65  VRR47 pKa = 11.84  SNVEE51 pKa = 3.79  SGFSIFTRR59 pKa = 11.84  DD60 pKa = 3.18  FLAPLRR66 pKa = 11.84  KK67 pKa = 9.18  RR68 pKa = 11.84  IHH70 pKa = 6.32  RR71 pKa = 11.84  RR72 pKa = 11.84  RR73 pKa = 11.84  KK74 pKa = 7.27  TEE76 pKa = 3.61  AFARR80 pKa = 11.84  TCDD83 pKa = 3.73  YY84 pKa = 11.17  NLKK87 pKa = 7.93  QACYY91 pKa = 10.14  ARR93 pKa = 11.84  HH94 pKa = 5.27  QEE96 pKa = 4.06  GLIAPWMNTT105 pKa = 2.93  
 12.61 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.344 
IPC2_protein 9.677 
IPC_protein 10.584 
Toseland    10.57 
ProMoST     10.335 
Dawson      10.701 
Bjellqvist  10.452 
Wikipedia   10.935 
Rodwell     10.833 
Grimsley    10.774 
Solomon     10.818 
Lehninger   10.789 
Nozaki      10.57 
DTASelect   10.438 
Thurlkill   10.599 
EMBOSS      10.979 
Sillero     10.643 
Patrickios  10.57 
IPC_peptide 10.818 
IPC2_peptide  9.575 
IPC2.peptide.svr19  8.494 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        779 
0
779 
190618
50
1136
244.7
27.4
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        6.876 ± 0.093
0.965 ± 0.036
5.485 ± 0.081
9.471 ± 0.113
3.708 ± 0.061
7.659 ± 0.086
1.829 ± 0.038
7.209 ± 0.079
7.179 ± 0.113
9.752 ± 0.086
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.216 ± 0.035
3.346 ± 0.06
4.264 ± 0.054
2.459 ± 0.042
5.716 ± 0.083
6.025 ± 0.08
4.934 ± 0.058
6.974 ± 0.071
1.114 ± 0.036
2.817 ± 0.053
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here