Nostoc sp. T09

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Nostoc; unclassified Nostoc

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6607 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A252DWT8|A0A252DWT8_9NOSO Hrp-dependent type III effector protein OS=Nostoc sp. T09 OX=1932621 GN=BV372_19655 PE=3 SV=1
MM1 pKa = 7.64VDD3 pKa = 3.7DD4 pKa = 5.28FFRR7 pKa = 11.84KK8 pKa = 10.09AKK10 pKa = 10.54DD11 pKa = 3.31LFLGRR16 pKa = 11.84NDD18 pKa = 3.51NQAEE22 pKa = 4.14EE23 pKa = 3.93EE24 pKa = 4.4DD25 pKa = 3.77YY26 pKa = 11.01RR27 pKa = 11.84DD28 pKa = 3.65RR29 pKa = 11.84QVRR32 pKa = 11.84PASEE36 pKa = 4.53DD37 pKa = 3.75PYY39 pKa = 11.5GDD41 pKa = 3.9PADD44 pKa = 3.44QNFYY48 pKa = 11.58GNVTPASQDD57 pKa = 3.43PYY59 pKa = 11.3GDD61 pKa = 3.66PADD64 pKa = 4.4FYY66 pKa = 11.75GNAIPASQDD75 pKa = 3.3PYY77 pKa = 11.39GDD79 pKa = 3.66PADD82 pKa = 4.4FYY84 pKa = 11.75GNAIPASQDD93 pKa = 3.3PYY95 pKa = 11.44GDD97 pKa = 4.23PADD100 pKa = 3.5QGYY103 pKa = 11.08GDD105 pKa = 4.38VRR107 pKa = 11.84PASEE111 pKa = 5.18DD112 pKa = 3.79PYY114 pKa = 11.34GDD116 pKa = 3.85PADD119 pKa = 3.62EE120 pKa = 4.41EE121 pKa = 4.57YY122 pKa = 10.9RR123 pKa = 11.84RR124 pKa = 3.86

Molecular weight: 13.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A252CRB8|A0A252CRB8_9NOSO Cytochrome c6 OS=Nostoc sp. T09 OX=1932621 GN=petJ PE=3 SV=1
MM1 pKa = 6.9WTWLWAWLWTWLWTWLRR18 pKa = 11.84IWLWAWLRR26 pKa = 11.84TWLRR30 pKa = 11.84TWLRR34 pKa = 11.84LWLRR38 pKa = 11.84IMLRR42 pKa = 11.84AWLRR46 pKa = 11.84TWLRR50 pKa = 11.84TWLRR54 pKa = 11.84LWLWIMLWTRR64 pKa = 11.84LWW66 pKa = 3.4

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6607

0

6607

2116477

29

2618

320.3

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.371 ± 0.035

0.954 ± 0.009

4.797 ± 0.021

6.204 ± 0.027

3.917 ± 0.02

6.485 ± 0.027

1.864 ± 0.014

6.812 ± 0.019

4.836 ± 0.025

11.084 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.774 ± 0.012

4.397 ± 0.024

4.669 ± 0.023

5.639 ± 0.029

5.073 ± 0.025

6.366 ± 0.024

5.61 ± 0.018

6.625 ± 0.022

1.449 ± 0.013

3.073 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski