Klebsiella phage Kp2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; Klebsiella virus Kp2

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0IXR8|A0A0P0IXR8_9CAUD Uncharacterized protein OS=Klebsiella phage Kp2 OX=1701805 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.37AILLCPEE9 pKa = 3.94HH10 pKa = 6.68KK11 pKa = 10.14LWYY14 pKa = 9.19LWCNQTCDD22 pKa = 3.48EE23 pKa = 4.32YY24 pKa = 11.34NYY26 pKa = 10.12TVVIFSDD33 pKa = 3.23RR34 pKa = 11.84DD35 pKa = 3.75TNQEE39 pKa = 3.96SPLEE43 pKa = 4.01FVDD46 pKa = 4.74TYY48 pKa = 11.4TEE50 pKa = 3.89MAARR54 pKa = 11.84TVLDD58 pKa = 3.67AVNLGSINDD67 pKa = 3.29WRR69 pKa = 11.84QLYY72 pKa = 9.72GG73 pKa = 3.34

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0IK20|A0A0P0IK20_9CAUD Uncharacterized protein OS=Klebsiella phage Kp2 OX=1701805 PE=4 SV=1
MM1 pKa = 7.13YY2 pKa = 9.86GKK4 pKa = 10.22SPEE7 pKa = 4.04TLLMRR12 pKa = 11.84KK13 pKa = 7.52QQQTIDD19 pKa = 3.49GLARR23 pKa = 11.84EE24 pKa = 4.55YY25 pKa = 10.64SAKK28 pKa = 10.43AALRR32 pKa = 11.84QHH34 pKa = 6.56YY35 pKa = 8.44EE36 pKa = 3.71RR37 pKa = 11.84QAQRR41 pKa = 11.84LGMTLRR47 pKa = 11.84GYY49 pKa = 9.38CVRR52 pKa = 11.84FNVRR56 pKa = 11.84GVVV59 pKa = 3.11

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

13805

37

1232

238.0

26.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.46 ± 0.6

1.145 ± 0.2

6.338 ± 0.208

5.882 ± 0.39

3.137 ± 0.192

7.381 ± 0.271

1.63 ± 0.159

4.411 ± 0.232

4.853 ± 0.326

8.721 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.898 ± 0.229

3.81 ± 0.213

3.781 ± 0.243

4.868 ± 0.376

5.809 ± 0.296

6.49 ± 0.322

5.991 ± 0.366

7.07 ± 0.237

1.463 ± 0.109

3.861 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski