Tortoise microvirus 100

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8WAE0|A0A4P8WAE0_9VIRU Uncharacterized protein OS=Tortoise microvirus 100 OX=2583100 PE=4 SV=1
MM1 pKa = 7.8ANNKK5 pKa = 9.53EE6 pKa = 4.24YY7 pKa = 10.2SLKK10 pKa = 10.61MIDD13 pKa = 3.66EE14 pKa = 4.21SRR16 pKa = 11.84KK17 pKa = 9.6ISAEE21 pKa = 3.87KK22 pKa = 10.44LDD24 pKa = 3.85YY25 pKa = 10.64TPIEE29 pKa = 4.22IPVSHH34 pKa = 6.5NVPEE38 pKa = 4.47TFNNTIQKK46 pKa = 9.53ILYY49 pKa = 7.98SSGMISQEE57 pKa = 4.36AYY59 pKa = 10.77HH60 pKa = 6.77KK61 pKa = 10.16MLGINYY67 pKa = 9.58DD68 pKa = 3.7GEE70 pKa = 5.0GDD72 pKa = 4.33GDD74 pKa = 4.66FDD76 pKa = 7.08DD77 pKa = 6.73DD78 pKa = 5.27DD79 pKa = 6.63DD80 pKa = 5.12EE81 pKa = 6.12FDD83 pKa = 3.59EE84 pKa = 5.52VYY86 pKa = 10.17EE87 pKa = 4.13QSPHH91 pKa = 5.93ASYY94 pKa = 11.6DD95 pKa = 3.4EE96 pKa = 4.21GRR98 pKa = 11.84KK99 pKa = 9.12SAEE102 pKa = 4.06VVEE105 pKa = 4.34NTPVEE110 pKa = 4.35TPKK113 pKa = 11.07GGEE116 pKa = 4.06TGNASPGSVDD126 pKa = 3.62PEE128 pKa = 4.16PVVDD132 pKa = 3.92TQPPKK137 pKa = 10.24NAEE140 pKa = 4.28KK141 pKa = 10.5KK142 pKa = 9.96SSSNKK147 pKa = 6.9TT148 pKa = 3.18

Molecular weight: 16.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8WAE0|A0A4P8WAE0_9VIRU Uncharacterized protein OS=Tortoise microvirus 100 OX=2583100 PE=4 SV=1
MM1 pKa = 7.44WGLARR6 pKa = 11.84FRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84NRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84PEE18 pKa = 3.44KK19 pKa = 10.06TRR21 pKa = 11.84NEE23 pKa = 4.03VSNKK27 pKa = 9.31KK28 pKa = 10.02RR29 pKa = 11.84LSPLLLKK36 pKa = 10.66PKK38 pKa = 10.09LLNRR42 pKa = 11.84NIEE45 pKa = 4.36TFDD48 pKa = 3.69KK49 pKa = 11.08PQDD52 pKa = 3.6TTRR55 pKa = 11.84KK56 pKa = 9.42LGPRR60 pKa = 11.84LKK62 pKa = 10.03IWSTQNDD69 pKa = 2.84NRR71 pKa = 11.84QNFNNDD77 pKa = 2.74TFSSIRR83 pKa = 11.84NILQLSRR90 pKa = 11.84LIDD93 pKa = 3.57HH94 pKa = 7.07DD95 pKa = 4.66RR96 pKa = 11.84EE97 pKa = 4.01VCRR100 pKa = 11.84RR101 pKa = 11.84RR102 pKa = 11.84KK103 pKa = 7.28TRR105 pKa = 11.84KK106 pKa = 9.85KK107 pKa = 9.87IILKK111 pKa = 6.26MTHH114 pKa = 5.62GRR116 pKa = 11.84GLSVKK121 pKa = 9.25KK122 pKa = 10.81AKK124 pKa = 8.09WTADD128 pKa = 3.15SLINCKK134 pKa = 8.42EE135 pKa = 3.73

Molecular weight: 16.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1446

135

607

289.2

33.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.772 ± 1.012

0.83 ± 0.469

5.463 ± 0.575

5.118 ± 0.846

5.74 ± 0.804

5.602 ± 0.508

1.452 ± 0.357

5.463 ± 0.302

7.054 ± 1.354

8.091 ± 0.79

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.075 ± 0.356

7.192 ± 0.703

4.703 ± 0.451

4.564 ± 0.861

5.878 ± 1.592

8.299 ± 0.487

5.947 ± 1.317

5.463 ± 0.685

1.383 ± 0.201

4.91 ± 0.983
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski