Amino acid dipepetide frequency for Candidatus Accumulibacter sp. BA-91

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.221AlaAla: 16.221 ± 0.169	1.6AlaCys: 1.6 ± 0.039	6.474AlaAsp: 6.474 ± 0.075	6.997AlaGlu: 6.997 ± 0.078	3.816AlaPhe: 3.816 ± 0.064	10.642AlaGly: 10.642 ± 0.113	2.541AlaHis: 2.541 ± 0.05	5.73AlaIle: 5.73 ± 0.082	3.2AlaLys: 3.2 ± 0.063	13.276AlaLeu: 13.276 ± 0.124	2.908AlaMet: 2.908 ± 0.049	2.964AlaAsn: 2.964 ± 0.057	4.788AlaPro: 4.788 ± 0.082	4.559AlaGln: 4.559 ± 0.064	9.206AlaArg: 9.206 ± 0.085	6.442AlaSer: 6.442 ± 0.086	5.539AlaThr: 5.539 ± 0.084	8.317AlaVal: 8.317 ± 0.088	1.648AlaTrp: 1.648 ± 0.041	2.364AlaTyr: 2.364 ± 0.047	0.03AlaXaa: 0.03 ± 0.006
Cys	1.266CysAla: 1.266 ± 0.036	0.242CysCys: 0.242 ± 0.015	0.604CysAsp: 0.604 ± 0.022	0.631CysGlu: 0.631 ± 0.024	0.459CysPhe: 0.459 ± 0.019	1.174CysGly: 1.174 ± 0.032	0.364CysHis: 0.364 ± 0.017	0.455CysIle: 0.455 ± 0.021	0.29CysLys: 0.29 ± 0.017	1.189CysLeu: 1.189 ± 0.034	0.228CysMet: 0.228 ± 0.014	0.279CysAsn: 0.279 ± 0.017	0.633CysPro: 0.633 ± 0.025	0.454CysGln: 0.454 ± 0.021	1.153CysArg: 1.153 ± 0.033	0.764CysSer: 0.764 ± 0.025	0.524CysThr: 0.524 ± 0.022	0.725CysVal: 0.725 ± 0.024	0.189CysTrp: 0.189 ± 0.011	0.271CysTyr: 0.271 ± 0.016	0.004CysXaa: 0.004 ± 0.002
Asp	6.435AspAla: 6.435 ± 0.084	0.624AspCys: 0.624 ± 0.024	3.296AspAsp: 3.296 ± 0.065	3.473AspGlu: 3.473 ± 0.057	2.339AspPhe: 2.339 ± 0.046	4.56AspGly: 4.56 ± 0.074	1.453AspHis: 1.453 ± 0.036	2.409AspIle: 2.409 ± 0.043	1.45AspLys: 1.45 ± 0.041	6.018AspLeu: 6.018 ± 0.066	0.889AspMet: 0.889 ± 0.027	1.209AspAsn: 1.209 ± 0.031	3.097AspPro: 3.097 ± 0.052	2.134AspGln: 2.134 ± 0.046	4.152AspArg: 4.152 ± 0.06	2.721AspSer: 2.721 ± 0.049	2.291AspThr: 2.291 ± 0.048	3.477AspVal: 3.477 ± 0.058	0.913AspTrp: 0.913 ± 0.028	1.41AspTyr: 1.41 ± 0.033	0.006AspXaa: 0.006 ± 0.002
Glu	6.638GluAla: 6.638 ± 0.089	0.533GluCys: 0.533 ± 0.024	2.342GluAsp: 2.342 ± 0.049	2.956GluGlu: 2.956 ± 0.061	2.009GluPhe: 2.009 ± 0.044	3.428GluGly: 3.428 ± 0.057	1.577GluHis: 1.577 ± 0.042	3.435GluIle: 3.435 ± 0.061	2.221GluLys: 2.221 ± 0.052	6.212GluLeu: 6.212 ± 0.082	1.483GluMet: 1.483 ± 0.04	1.673GluAsn: 1.673 ± 0.037	2.304GluPro: 2.304 ± 0.046	2.95GluGln: 2.95 ± 0.052	5.014GluArg: 5.014 ± 0.068	2.945GluSer: 2.945 ± 0.048	2.779GluThr: 2.779 ± 0.046	4.299GluVal: 4.299 ± 0.063	0.831GluTrp: 0.831 ± 0.028	1.136GluTyr: 1.136 ± 0.028	0.007GluXaa: 0.007 ± 0.002
Phe	4.43PheAla: 4.43 ± 0.059	0.461PheCys: 0.461 ± 0.02	2.44PheAsp: 2.44 ± 0.044	2.12PheGlu: 2.12 ± 0.047	1.465PhePhe: 1.465 ± 0.037	3.292PheGly: 3.292 ± 0.051	0.831PheHis: 0.831 ± 0.027	1.484PheIle: 1.484 ± 0.035	0.92PheLys: 0.92 ± 0.027	3.663PheLeu: 3.663 ± 0.063	0.658PheMet: 0.658 ± 0.022	1.002PheAsn: 1.002 ± 0.03	1.675PhePro: 1.675 ± 0.037	1.111PheGln: 1.111 ± 0.03	2.364PheArg: 2.364 ± 0.044	2.326PheSer: 2.326 ± 0.049	1.728PheThr: 1.728 ± 0.042	2.766PheVal: 2.766 ± 0.052	0.457PheTrp: 0.457 ± 0.022	0.834PheTyr: 0.834 ± 0.029	0.008PheXaa: 0.008 ± 0.003
Gly	7.464GlyAla: 7.464 ± 0.087	1.066GlyCys: 1.066 ± 0.034	4.409GlyAsp: 4.409 ± 0.066	4.893GlyGlu: 4.893 ± 0.06	3.167GlyPhe: 3.167 ± 0.058	6.306GlyGly: 6.306 ± 0.087	1.973GlyHis: 1.973 ± 0.042	4.354GlyIle: 4.354 ± 0.064	3.429GlyLys: 3.429 ± 0.061	8.184GlyLeu: 8.184 ± 0.084	2.133GlyMet: 2.133 ± 0.035	2.55GlyAsn: 2.55 ± 0.061	2.509GlyPro: 2.509 ± 0.049	3.536GlyGln: 3.536 ± 0.067	5.872GlyArg: 5.872 ± 0.084	5.03GlySer: 5.03 ± 0.089	3.907GlyThr: 3.907 ± 0.07	5.957GlyVal: 5.957 ± 0.082	1.232GlyTrp: 1.232 ± 0.037	1.921GlyTyr: 1.921 ± 0.046	0.021GlyXaa: 0.021 ± 0.005
His	2.885HisAla: 2.885 ± 0.06	0.393HisCys: 0.393 ± 0.018	1.388HisAsp: 1.388 ± 0.036	1.191HisGlu: 1.191 ± 0.032	1.079HisPhe: 1.079 ± 0.033	1.955HisGly: 1.955 ± 0.04	0.834HisHis: 0.834 ± 0.032	0.909HisIle: 0.909 ± 0.026	0.502HisLys: 0.502 ± 0.021	2.876HisLeu: 2.876 ± 0.053	0.378HisMet: 0.378 ± 0.021	0.426HisAsn: 0.426 ± 0.022	1.621HisPro: 1.621 ± 0.04	1.104HisGln: 1.104 ± 0.032	2.111HisArg: 2.111 ± 0.045	1.191HisSer: 1.191 ± 0.029	0.937HisThr: 0.937 ± 0.03	1.504HisVal: 1.504 ± 0.035	0.373HisTrp: 0.373 ± 0.018	0.686HisTyr: 0.686 ± 0.027	0.005HisXaa: 0.005 ± 0.002
Ile	6.132IleAla: 6.132 ± 0.08	0.533IleCys: 0.533 ± 0.019	3.66IleAsp: 3.66 ± 0.063	3.555IleGlu: 3.555 ± 0.06	1.484IlePhe: 1.484 ± 0.038	4.466IleGly: 4.466 ± 0.063	1.042IleHis: 1.042 ± 0.029	1.872IleIle: 1.872 ± 0.042	1.35IleLys: 1.35 ± 0.039	4.237IleLeu: 4.237 ± 0.064	0.695IleMet: 0.695 ± 0.026	1.352IleAsn: 1.352 ± 0.035	2.245IlePro: 2.245 ± 0.048	1.334IleGln: 1.334 ± 0.034	3.284IleArg: 3.284 ± 0.055	2.666IleSer: 2.666 ± 0.054	2.32IleThr: 2.32 ± 0.049	3.696IleVal: 3.696 ± 0.056	0.455IleTrp: 0.455 ± 0.02	0.989IleTyr: 0.989 ± 0.026	0.008IleXaa: 0.008 ± 0.003
Lys	3.351LysAla: 3.351 ± 0.057	0.222LysCys: 0.222 ± 0.013	1.498LysAsp: 1.498 ± 0.039	1.553LysGlu: 1.553 ± 0.045	0.826LysPhe: 0.826 ± 0.026	2.161LysGly: 2.161 ± 0.048	0.682LysHis: 0.682 ± 0.023	1.522LysIle: 1.522 ± 0.043	1.175LysLys: 1.175 ± 0.041	3.278LysLeu: 3.278 ± 0.06	0.711LysMet: 0.711 ± 0.029	0.881LysAsn: 0.881 ± 0.031	1.883LysPro: 1.883 ± 0.049	1.179LysGln: 1.179 ± 0.033	2.299LysArg: 2.299 ± 0.054	1.64LysSer: 1.64 ± 0.039	1.72LysThr: 1.72 ± 0.048	2.182LysVal: 2.182 ± 0.053	0.326LysTrp: 0.326 ± 0.02	0.627LysTyr: 0.627 ± 0.025	0.003LysXaa: 0.003 ± 0.002
Leu	15.427LeuAla: 15.427 ± 0.139	1.324LeuCys: 1.324 ± 0.033	6.277LeuAsp: 6.277 ± 0.076	5.681LeuGlu: 5.681 ± 0.072	3.787LeuPhe: 3.787 ± 0.057	8.271LeuGly: 8.271 ± 0.1	2.443LeuHis: 2.443 ± 0.045	4.855LeuIle: 4.855 ± 0.078	3.209LeuLys: 3.209 ± 0.06	13.578LeuLeu: 13.578 ± 0.167	2.065LeuMet: 2.065 ± 0.043	2.631LeuAsn: 2.631 ± 0.048	6.655LeuPro: 6.655 ± 0.085	4.555LeuGln: 4.555 ± 0.068	8.717LeuArg: 8.717 ± 0.102	6.331LeuSer: 6.331 ± 0.074	5.315LeuThr: 5.315 ± 0.078	7.411LeuVal: 7.411 ± 0.083	1.343LeuTrp: 1.343 ± 0.034	2.114LeuTyr: 2.114 ± 0.052	0.022LeuXaa: 0.022 ± 0.004
Met	2.631MetAla: 2.631 ± 0.05	0.167MetCys: 0.167 ± 0.012	0.941MetAsp: 0.941 ± 0.026	0.904MetGlu: 0.904 ± 0.03	0.647MetPhe: 0.647 ± 0.024	1.292MetGly: 1.292 ± 0.036	0.578MetHis: 0.578 ± 0.021	1.089MetIle: 1.089 ± 0.033	0.831MetLys: 0.831 ± 0.029	2.554MetLeu: 2.554 ± 0.056	0.478MetMet: 0.478 ± 0.022	0.796MetAsn: 0.796 ± 0.026	1.363MetPro: 1.363 ± 0.032	0.982MetGln: 0.982 ± 0.029	1.632MetArg: 1.632 ± 0.038	1.348MetSer: 1.348 ± 0.035	1.294MetThr: 1.294 ± 0.036	1.435MetVal: 1.435 ± 0.036	0.173MetTrp: 0.173 ± 0.013	0.304MetTyr: 0.304 ± 0.016	0.002MetXaa: 0.002 ± 0.001
Asn	2.892AsnAla: 2.892 ± 0.055	0.304AsnCys: 0.304 ± 0.014	1.426AsnAsp: 1.426 ± 0.038	1.342AsnGlu: 1.342 ± 0.035	0.943AsnPhe: 0.943 ± 0.027	2.232AsnGly: 2.232 ± 0.048	0.617AsnHis: 0.617 ± 0.022	1.181AsnIle: 1.181 ± 0.033	0.722AsnLys: 0.722 ± 0.025	2.777AsnLeu: 2.777 ± 0.051	0.453AsnMet: 0.453 ± 0.018	0.668AsnAsn: 0.668 ± 0.026	1.808AsnPro: 1.808 ± 0.045	0.929AsnGln: 0.929 ± 0.032	2.049AsnArg: 2.049 ± 0.042	1.345AsnSer: 1.345 ± 0.034	1.288AsnThr: 1.288 ± 0.039	1.722AsnVal: 1.722 ± 0.048	0.428AsnTrp: 0.428 ± 0.019	0.6AsnTyr: 0.6 ± 0.024	0.003AsnXaa: 0.003 ± 0.001
Pro	6.668ProAla: 6.668 ± 0.103	0.52ProCys: 0.52 ± 0.022	3.089ProAsp: 3.089 ± 0.048	3.494ProGlu: 3.494 ± 0.059	1.755ProPhe: 1.755 ± 0.037	4.498ProGly: 4.498 ± 0.066	0.983ProHis: 0.983 ± 0.03	1.927ProIle: 1.927 ± 0.04	1.276ProLys: 1.276 ± 0.036	5.457ProLeu: 5.457 ± 0.068	1.117ProMet: 1.117 ± 0.028	1.117ProAsn: 1.117 ± 0.032	2.89ProPro: 2.89 ± 0.064	2.024ProGln: 2.024 ± 0.048	3.113ProArg: 3.113 ± 0.05	2.584ProSer: 2.584 ± 0.046	2.282ProThr: 2.282 ± 0.044	3.967ProVal: 3.967 ± 0.06	0.726ProTrp: 0.726 ± 0.025	1.118ProTyr: 1.118 ± 0.03	0.016ProXaa: 0.016 ± 0.003
Gln	5.336GlnAla: 5.336 ± 0.071	0.413GlnCys: 0.413 ± 0.024	1.605GlnAsp: 1.605 ± 0.035	1.988GlnGlu: 1.988 ± 0.045	1.335GlnPhe: 1.335 ± 0.04	3.106GlnGly: 3.106 ± 0.053	1.145GlnHis: 1.145 ± 0.037	2.138GlnIle: 2.138 ± 0.042	1.167GlnLys: 1.167 ± 0.033	4.407GlnLeu: 4.407 ± 0.07	0.999GlnMet: 0.999 ± 0.027	0.896GlnAsn: 0.896 ± 0.027	2.249GlnPro: 2.249 ± 0.053	2.418GlnGln: 2.418 ± 0.054	3.981GlnArg: 3.981 ± 0.064	2.034GlnSer: 2.034 ± 0.044	1.994GlnThr: 1.994 ± 0.044	3.119GlnVal: 3.119 ± 0.054	0.55GlnTrp: 0.55 ± 0.023	0.748GlnTyr: 0.748 ± 0.025	0.008GlnXaa: 0.008 ± 0.003
Arg	7.546ArgAla: 7.546 ± 0.09	1.058ArgCys: 1.058 ± 0.038	3.747ArgAsp: 3.747 ± 0.054	4.517ArgGlu: 4.517 ± 0.071	3.139ArgPhe: 3.139 ± 0.052	4.658ArgGly: 4.658 ± 0.069	2.389ArgHis: 2.389 ± 0.055	4.157ArgIle: 4.157 ± 0.061	2.395ArgLys: 2.395 ± 0.047	9.537ArgLeu: 9.537 ± 0.107	1.892ArgMet: 1.892 ± 0.044	2.041ArgAsn: 2.041 ± 0.043	3.501ArgPro: 3.501 ± 0.057	4.415ArgGln: 4.415 ± 0.077	6.767ArgArg: 6.767 ± 0.101	4.329ArgSer: 4.329 ± 0.059	3.229ArgThr: 3.229 ± 0.054	5.296ArgVal: 5.296 ± 0.062	1.159ArgTrp: 1.159 ± 0.035	1.984ArgTyr: 1.984 ± 0.042	0.02ArgXaa: 0.02 ± 0.004
Ser	6.169SerAla: 6.169 ± 0.083	0.6SerCys: 0.6 ± 0.026	2.924SerAsp: 2.924 ± 0.053	3.135SerGlu: 3.135 ± 0.056	2.007SerPhe: 2.007 ± 0.041	5.439SerGly: 5.439 ± 0.08	1.23SerHis: 1.23 ± 0.033	2.628SerIle: 2.628 ± 0.052	1.583SerLys: 1.583 ± 0.038	6.067SerLeu: 6.067 ± 0.078	1.288SerMet: 1.288 ± 0.035	1.377SerAsn: 1.377 ± 0.036	3.058SerPro: 3.058 ± 0.057	1.959SerGln: 1.959 ± 0.045	4.138SerArg: 4.138 ± 0.052	3.577SerSer: 3.577 ± 0.077	2.941SerThr: 2.941 ± 0.067	3.777SerVal: 3.777 ± 0.064	0.669SerTrp: 0.669 ± 0.026	1.301SerTyr: 1.301 ± 0.039	0.013SerXaa: 0.013 ± 0.003
Thr	5.624ThrAla: 5.624 ± 0.088	0.461ThrCys: 0.461 ± 0.023	2.456ThrAsp: 2.456 ± 0.045	2.203ThrGlu: 2.203 ± 0.046	1.641ThrPhe: 1.641 ± 0.037	4.36ThrGly: 4.36 ± 0.072	1.103ThrHis: 1.103 ± 0.03	2.275ThrIle: 2.275 ± 0.044	1.01ThrLys: 1.01 ± 0.033	6.077ThrLeu: 6.077 ± 0.08	0.91ThrMet: 0.91 ± 0.029	1.067ThrAsn: 1.067 ± 0.033	3.162ThrPro: 3.162 ± 0.058	1.694ThrGln: 1.694 ± 0.042	3.523ThrArg: 3.523 ± 0.049	2.48ThrSer: 2.48 ± 0.053	2.469ThrThr: 2.469 ± 0.057	3.657ThrVal: 3.657 ± 0.06	0.58ThrTrp: 0.58 ± 0.021	0.915ThrTyr: 0.915 ± 0.029	0.019ThrXaa: 0.019 ± 0.004
Val	8.713ValAla: 8.713 ± 0.091	0.868ValCys: 0.868 ± 0.028	4.156ValAsp: 4.156 ± 0.067	4.475ValGlu: 4.475 ± 0.078	2.76ValPhe: 2.76 ± 0.048	5.535ValGly: 5.535 ± 0.084	1.606ValHis: 1.606 ± 0.039	3.579ValIle: 3.579 ± 0.052	2.02ValLys: 2.02 ± 0.048	7.936ValLeu: 7.936 ± 0.087	1.513ValMet: 1.513 ± 0.042	1.885ValAsn: 1.885 ± 0.043	3.266ValPro: 3.266 ± 0.059	2.436ValGln: 2.436 ± 0.05	4.981ValArg: 4.981 ± 0.063	4.173ValSer: 4.173 ± 0.07	3.585ValThr: 3.585 ± 0.059	5.725ValVal: 5.725 ± 0.081	0.855ValTrp: 0.855 ± 0.03	1.306ValTyr: 1.306 ± 0.036	0.024ValXaa: 0.024 ± 0.004
Trp	1.036TrpAla: 1.036 ± 0.03	0.194TrpCys: 0.194 ± 0.013	0.489TrpAsp: 0.489 ± 0.022	0.626TrpGlu: 0.626 ± 0.022	0.493TrpPhe: 0.493 ± 0.024	0.764TrpGly: 0.764 ± 0.026	0.377TrpHis: 0.377 ± 0.017	0.644TrpIle: 0.644 ± 0.026	0.466TrpLys: 0.466 ± 0.02	2.005TrpLeu: 2.005 ± 0.046	0.316TrpMet: 0.316 ± 0.016	0.399TrpAsn: 0.399 ± 0.017	0.716TrpPro: 0.716 ± 0.025	0.875TrpGln: 0.875 ± 0.028	1.33TrpArg: 1.33 ± 0.034	0.775TrpSer: 0.775 ± 0.027	0.572TrpThr: 0.572 ± 0.024	0.871TrpVal: 0.871 ± 0.029	0.237TrpTrp: 0.237 ± 0.015	0.306TrpTyr: 0.306 ± 0.016	0.002TrpXaa: 0.002 ± 0.001
Tyr	2.374TyrAla: 2.374 ± 0.047	0.311TyrCys: 0.311 ± 0.015	1.128TyrAsp: 1.128 ± 0.037	1.027TyrGlu: 1.027 ± 0.029	0.881TyrPhe: 0.881 ± 0.029	1.752TyrGly: 1.752 ± 0.04	0.568TyrHis: 0.568 ± 0.02	0.72TyrIle: 0.72 ± 0.025	0.495TyrLys: 0.495 ± 0.023	2.671TyrLeu: 2.671 ± 0.048	0.318TyrMet: 0.318 ± 0.017	0.514TyrAsn: 0.514 ± 0.023	1.181TyrPro: 1.181 ± 0.033	0.977TyrGln: 0.977 ± 0.029	2.072TyrArg: 2.072 ± 0.047	1.16TyrSer: 1.16 ± 0.032	0.962TyrThr: 0.962 ± 0.03	1.478TyrVal: 1.478 ± 0.033	0.345TyrTrp: 0.345 ± 0.017	0.59TyrTyr: 0.59 ± 0.027	0.003TyrXaa: 0.003 ± 0.002
Xaa	0.024XaaAla: 0.024 ± 0.005	0.005XaaCys: 0.005 ± 0.002	0.005XaaAsp: 0.005 ± 0.002	0.009XaaGlu: 0.009 ± 0.003	0.003XaaPhe: 0.003 ± 0.002	0.023XaaGly: 0.023 ± 0.004	0.013XaaHis: 0.013 ± 0.003	0.01XaaIle: 0.01 ± 0.003	0.006XaaLys: 0.006 ± 0.002	0.016XaaLeu: 0.016 ± 0.004	0.008XaaMet: 0.008 ± 0.003	0.003XaaAsn: 0.003 ± 0.002	0.018XaaPro: 0.018 ± 0.004	0.01XaaGln: 0.01 ± 0.003	0.018XaaArg: 0.018 ± 0.004	0.018XaaSer: 0.018 ± 0.004	0.012XaaThr: 0.012 ± 0.003	0.013XaaVal: 0.013 ± 0.003	0.005XaaTrp: 0.005 ± 0.002	0.005XaaTyr: 0.005 ± 0.002	0.897XaaXaa: 0.897 ± 0.087

Statistics based on 4449 proteins (1187751 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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