Lysobacter sp. A03

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; unclassified Lysobacter

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D0S020|A0A0D0S020_9GAMM NODE_37 whole genome shotgun sequence OS=Lysobacter sp. A03 OX=1199154 GN=TI01_1212 PE=4 SV=1
MM1 pKa = 7.05GTTGGEE7 pKa = 3.79RR8 pKa = 11.84QIVNVADD15 pKa = 3.43ATVATDD21 pKa = 3.9AVNLRR26 pKa = 11.84QMQSAIAGVGGVTMPQVQTVVDD48 pKa = 3.9AGDD51 pKa = 3.66AQTLADD57 pKa = 3.83ANAYY61 pKa = 8.17TDD63 pKa = 3.53SQIIAAGSITPAQVQAIADD82 pKa = 3.84AGDD85 pKa = 3.61AQTLTDD91 pKa = 3.68ANAYY95 pKa = 8.11TDD97 pKa = 3.95ASAAQTLADD106 pKa = 3.87ANSYY110 pKa = 9.36TDD112 pKa = 4.15AGTTQTLADD121 pKa = 3.64ANAYY125 pKa = 8.44TDD127 pKa = 3.61ASSAQTLTDD136 pKa = 3.42AYY138 pKa = 9.38TYY140 pKa = 9.73TDD142 pKa = 3.33SGTAQALADD151 pKa = 3.64AKK153 pKa = 10.56IYY155 pKa = 10.51IDD157 pKa = 3.77AQVISAGSITEE168 pKa = 4.05NQVQAIADD176 pKa = 3.8AGDD179 pKa = 3.61AQTLTDD185 pKa = 3.68ANAYY189 pKa = 10.31SDD191 pKa = 4.11AGDD194 pKa = 3.69VQTLADD200 pKa = 3.66ANAYY204 pKa = 10.38SDD206 pKa = 4.97AGDD209 pKa = 3.84TQTLASANAYY219 pKa = 7.66TDD221 pKa = 3.46SGDD224 pKa = 3.81ARR226 pKa = 11.84TLSDD230 pKa = 3.05ARR232 pKa = 11.84AYY234 pKa = 8.01TATTATQTLQTANTYY249 pKa = 11.35ADD251 pKa = 3.55TGDD254 pKa = 3.63AATLQSANAYY264 pKa = 8.22TDD266 pKa = 3.4QQVARR271 pKa = 11.84FNHH274 pKa = 5.95GLDD277 pKa = 3.57EE278 pKa = 4.82FRR280 pKa = 11.84MEE282 pKa = 3.87MDD284 pKa = 4.61DD285 pKa = 5.09RR286 pKa = 11.84FHH288 pKa = 6.31TLDD291 pKa = 3.51RR292 pKa = 11.84RR293 pKa = 11.84IDD295 pKa = 3.5RR296 pKa = 11.84MGATSAAYY304 pKa = 9.93AGLAANTAGLGGANNIGVGIGSQGGQQALAIGYY337 pKa = 8.89RR338 pKa = 11.84RR339 pKa = 11.84AIGARR344 pKa = 11.84ASVSLGGAIAGGEE357 pKa = 4.3SSVSAGAGFSWW368 pKa = 3.78

Molecular weight: 37.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D0R6J3|A0A0D0R6J3_9GAMM NODE_17 whole genome shotgun sequence OS=Lysobacter sp. A03 OX=1199154 GN=TI01_1184 PE=4 SV=1
MM1 pKa = 7.41VAIQQGLRR9 pKa = 11.84EE10 pKa = 4.54DD11 pKa = 4.1GFDD14 pKa = 3.17VSMVKK19 pKa = 10.33LCRR22 pKa = 11.84WFGVARR28 pKa = 11.84RR29 pKa = 11.84TVYY32 pKa = 10.28YY33 pKa = 10.06RR34 pKa = 11.84PTKK37 pKa = 10.33APSTVRR43 pKa = 11.84PEE45 pKa = 3.51LAEE48 pKa = 4.61PIKK51 pKa = 10.77ALIEE55 pKa = 4.15AEE57 pKa = 4.03PSFGYY62 pKa = 8.7RR63 pKa = 11.84TVAGLLGMNKK73 pKa = 8.28NTVQRR78 pKa = 11.84IFQLKK83 pKa = 7.71GWQVRR88 pKa = 11.84KK89 pKa = 9.35RR90 pKa = 11.84AVGRR94 pKa = 11.84RR95 pKa = 11.84PRR97 pKa = 11.84IEE99 pKa = 4.42ALPSVAQAPDD109 pKa = 3.64EE110 pKa = 4.13RR111 pKa = 11.84WATDD115 pKa = 3.24LCRR118 pKa = 11.84IWGGRR123 pKa = 11.84DD124 pKa = 2.88GWLTLALVIDD134 pKa = 4.07CHH136 pKa = 6.05TRR138 pKa = 11.84QLLGWQLSRR147 pKa = 11.84SGRR150 pKa = 11.84ATTAAAALEE159 pKa = 4.03QALITRR165 pKa = 11.84YY166 pKa = 8.15GTLGRR171 pKa = 11.84VPAPFLLRR179 pKa = 11.84SDD181 pKa = 3.62NGLVFTSRR189 pKa = 11.84HH190 pKa = 3.84YY191 pKa = 9.24TRR193 pKa = 11.84LVRR196 pKa = 11.84SYY198 pKa = 11.13GLKK201 pKa = 9.89QEE203 pKa = 5.56FITPHH208 pKa = 6.56CPQQNGMVEE217 pKa = 3.99RR218 pKa = 11.84VIRR221 pKa = 11.84TLKK224 pKa = 8.41EE225 pKa = 3.75QCAHH229 pKa = 5.3RR230 pKa = 11.84HH231 pKa = 4.58RR232 pKa = 11.84FEE234 pKa = 4.28TLQHH238 pKa = 5.6AMRR241 pKa = 11.84VIGDD245 pKa = 4.3WIQFYY250 pKa = 7.98NHH252 pKa = 6.68RR253 pKa = 11.84RR254 pKa = 11.84PHH256 pKa = 5.38QALGMKK262 pKa = 9.24TPAEE266 pKa = 4.29AYY268 pKa = 10.2ALAII272 pKa = 4.36

Molecular weight: 31.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2559

0

2559

836927

37

2905

327.1

35.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.641 ± 0.066

0.788 ± 0.012

6.178 ± 0.038

5.552 ± 0.047

3.325 ± 0.034

8.674 ± 0.05

2.245 ± 0.024

4.389 ± 0.035

2.74 ± 0.044

10.472 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.394 ± 0.024

2.5 ± 0.026

5.286 ± 0.036

3.583 ± 0.026

7.461 ± 0.048

5.488 ± 0.036

4.984 ± 0.031

7.602 ± 0.04

1.457 ± 0.025

2.24 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski