Bacillus sp. FJAT-27231

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3828 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K9H9X6|A0A0K9H9X6_9BACI Octanoyl-[GcvH]:protein N-octanoyltransferase OS=Bacillus sp. FJAT-27231 OX=1679168 GN=lipL PE=3 SV=1
MM1 pKa = 8.01DD2 pKa = 4.55CVNWFIEE9 pKa = 5.2GYY11 pKa = 10.21KK12 pKa = 10.37KK13 pKa = 10.18LTGKK17 pKa = 7.64TICAEE22 pKa = 3.98QVGYY26 pKa = 10.2EE27 pKa = 4.05AVEE30 pKa = 3.93LAQEE34 pKa = 4.22EE35 pKa = 4.26IGYY38 pKa = 9.68EE39 pKa = 4.19FIPEE43 pKa = 3.64EE44 pKa = 4.49DD45 pKa = 3.73LRR47 pKa = 11.84KK48 pKa = 10.15LPDD51 pKa = 4.05PLLVEE56 pKa = 4.07TCVYY60 pKa = 11.07DD61 pKa = 4.83DD62 pKa = 4.01EE63 pKa = 6.3NGNEE67 pKa = 4.06WIAGIVLCGDD77 pKa = 4.54LNAGIYY83 pKa = 7.37TFWTRR88 pKa = 11.84NGEE91 pKa = 4.33GISRR95 pKa = 11.84QFTSEE100 pKa = 3.48WGGEE104 pKa = 4.23GLL106 pKa = 4.87

Molecular weight: 11.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K9HBR2|A0A0K9HBR2_9BACI Glyoxalase OS=Bacillus sp. FJAT-27231 OX=1679168 GN=AC623_20200 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSKK25 pKa = 10.18NGRR28 pKa = 11.84RR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3828

0

3828

1141752

37

1849

298.3

33.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.954 ± 0.046

0.717 ± 0.011

4.693 ± 0.03

7.732 ± 0.048

4.568 ± 0.034

7.076 ± 0.039

2.109 ± 0.018

7.49 ± 0.036

6.775 ± 0.033

9.853 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.821 ± 0.019

3.906 ± 0.027

3.75 ± 0.023

3.796 ± 0.03

4.173 ± 0.029

5.889 ± 0.028

5.314 ± 0.024

7.064 ± 0.036

1.017 ± 0.015

3.303 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski