Seal anellovirus 2
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V5NF43|V5NF43_9VIRU ORF2 OS=Seal anellovirus 2 OX=1427157 PE=4 SV=1
MM1 pKa = 7.71 SADD4 pKa = 3.85 CDD6 pKa = 3.96 SARR9 pKa = 11.84 GPDD12 pKa = 3.44 LCHH15 pKa = 6.06 PQLYY19 pKa = 9.75 KK20 pKa = 10.99 YY21 pKa = 10.59 KK22 pKa = 9.05 EE23 pKa = 4.38 AKK25 pKa = 8.85 WKK27 pKa = 9.11 QQVSKK32 pKa = 10.24 LHH34 pKa = 7.35 RR35 pKa = 11.84 EE36 pKa = 3.83 FCGCNNFLDD45 pKa = 4.05 HH46 pKa = 6.71 FKK48 pKa = 10.61 WPGTALPGGGDD59 pKa = 3.48 AGEE62 pKa = 4.65 GSQDD66 pKa = 3.41 GGDD69 pKa = 3.36 TAGIGGLSTTGGGGGDD85 pKa = 4.54 SITDD89 pKa = 3.57 MEE91 pKa = 4.71 LLRR94 pKa = 5.87
Molecular weight: 9.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.984
IPC2_protein 5.118
IPC_protein 4.965
Toseland 4.927
ProMoST 5.169
Dawson 5.016
Bjellqvist 5.13
Wikipedia 4.952
Rodwell 4.914
Grimsley 4.863
Solomon 5.016
Lehninger 4.978
Nozaki 5.169
DTASelect 5.385
Thurlkill 4.991
EMBOSS 5.016
Sillero 5.207
Patrickios 3.808
IPC_peptide 5.016
IPC2_peptide 5.207
IPC2.peptide.svr19 5.192
Protein with the highest isoelectric point:
>tr|V5NF43|V5NF43_9VIRU ORF2 OS=Seal anellovirus 2 OX=1427157 PE=4 SV=1
MM1 pKa = 7.36 ARR3 pKa = 11.84 HH4 pKa = 6.13 RR5 pKa = 11.84 FTWRR9 pKa = 11.84 GRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 FPRR18 pKa = 11.84 WRR20 pKa = 11.84 RR21 pKa = 11.84 YY22 pKa = 8.31 RR23 pKa = 11.84 RR24 pKa = 11.84 YY25 pKa = 8.97 WRR27 pKa = 11.84 PFHH30 pKa = 5.69 HH31 pKa = 6.18 WRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 FHH39 pKa = 6.66 HH40 pKa = 5.7 RR41 pKa = 11.84 HH42 pKa = 4.09 GTASVRR48 pKa = 11.84 YY49 pKa = 7.88 WRR51 pKa = 11.84 SSRR54 pKa = 11.84 NKK56 pKa = 8.73 TIVVRR61 pKa = 11.84 GWEE64 pKa = 4.1 PLGNICPGSDD74 pKa = 3.07 ASKK77 pKa = 10.53 EE78 pKa = 3.98 ATPWEE83 pKa = 4.35 VLDD86 pKa = 5.4 KK87 pKa = 10.92 DD88 pKa = 4.31 YY89 pKa = 11.61 EE90 pKa = 4.22 DD91 pKa = 3.92 TKK93 pKa = 11.09 TKK95 pKa = 10.64 GKK97 pKa = 10.55 EE98 pKa = 3.66 SDD100 pKa = 3.83 CAKK103 pKa = 10.18 SCWSGTFGGHH113 pKa = 6.55 FFTFKK118 pKa = 11.06 LLLQRR123 pKa = 11.84 AKK125 pKa = 10.35 IRR127 pKa = 11.84 LCQFSSDD134 pKa = 3.52 WNGWDD139 pKa = 3.28 YY140 pKa = 11.98 LEE142 pKa = 5.08 FKK144 pKa = 10.45 GGYY147 pKa = 8.22 IWLPRR152 pKa = 11.84 LAGLQWMFNKK162 pKa = 10.17 DD163 pKa = 3.42 HH164 pKa = 6.85 SIKK167 pKa = 10.96 NPTEE171 pKa = 3.95 TKK173 pKa = 10.24 EE174 pKa = 4.21 GQEE177 pKa = 3.69 LAAAKK182 pKa = 9.91 SWTHH186 pKa = 5.69 PGVWLNRR193 pKa = 11.84 RR194 pKa = 11.84 GPIIMHH200 pKa = 7.04 DD201 pKa = 3.6 PNLFPSHH208 pKa = 6.71 RR209 pKa = 11.84 GFRR212 pKa = 11.84 RR213 pKa = 11.84 LKK215 pKa = 10.03 VKK217 pKa = 10.39 PPTGWEE223 pKa = 3.84 GHH225 pKa = 5.63 YY226 pKa = 10.71 SLPAVMNYY234 pKa = 9.38 IFFQWFWTALDD245 pKa = 4.09 MQHH248 pKa = 7.02 AMYY251 pKa = 10.25 DD252 pKa = 3.65 YY253 pKa = 10.16 WCVKK257 pKa = 9.83 QNPTAEE263 pKa = 4.2 HH264 pKa = 6.12 TKK266 pKa = 9.75 ICCQVPWWAAQIDD279 pKa = 4.39 DD280 pKa = 3.75 TWRR283 pKa = 11.84 PKK285 pKa = 10.76 YY286 pKa = 10.86 ADD288 pKa = 4.73 DD289 pKa = 6.16 DD290 pKa = 4.21 PAKK293 pKa = 9.98 TCGGDD298 pKa = 3.96 VRR300 pKa = 11.84 EE301 pKa = 4.25 LWTDD305 pKa = 3.59 RR306 pKa = 11.84 EE307 pKa = 4.33 NYY309 pKa = 9.63 LQSKK313 pKa = 10.29 CNTNQARR320 pKa = 11.84 TDD322 pKa = 3.6 QNNIPANTQNWGPFLPQSFVRR343 pKa = 11.84 PGEE346 pKa = 4.12 AGFSLWFKK354 pKa = 10.55 YY355 pKa = 10.22 KK356 pKa = 10.46 FYY358 pKa = 10.65 FQLSGDD364 pKa = 3.61 SVYY367 pKa = 10.61 RR368 pKa = 11.84 QQPHH372 pKa = 6.26 TDD374 pKa = 3.22 PKK376 pKa = 10.82 VVIPRR381 pKa = 11.84 APKK384 pKa = 10.38 AKK386 pKa = 8.81 NTLTGEE392 pKa = 4.25 VQPGPEE398 pKa = 4.11 RR399 pKa = 11.84 PQDD402 pKa = 3.3 PWDD405 pKa = 4.12 ILPGDD410 pKa = 4.5 LDD412 pKa = 3.29 RR413 pKa = 11.84 HH414 pKa = 6.26 GILKK418 pKa = 9.57 EE419 pKa = 3.41 RR420 pKa = 11.84 AYY422 pKa = 10.9 RR423 pKa = 11.84 RR424 pKa = 11.84 ITSTGPGDD432 pKa = 3.75 EE433 pKa = 4.14 PAKK436 pKa = 10.24 VGPSRR441 pKa = 11.84 VRR443 pKa = 11.84 FRR445 pKa = 11.84 SDD447 pKa = 2.72 PSVRR451 pKa = 11.84 RR452 pKa = 11.84 KK453 pKa = 9.64 RR454 pKa = 11.84 LRR456 pKa = 11.84 HH457 pKa = 4.86 ILSRR461 pKa = 11.84 LLEE464 pKa = 4.2
Molecular weight: 55.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.279
IPC2_protein 9.443
IPC_protein 9.706
Toseland 10.248
ProMoST 9.911
Dawson 10.409
Bjellqvist 10.101
Wikipedia 10.584
Rodwell 10.73
Grimsley 10.467
Solomon 10.452
Lehninger 10.423
Nozaki 10.262
DTASelect 10.087
Thurlkill 10.277
EMBOSS 10.643
Sillero 10.335
Patrickios 10.335
IPC_peptide 10.452
IPC2_peptide 8.99
IPC2.peptide.svr19 8.478
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
762
94
464
254.0
29.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.856 ± 0.775
1.969 ± 0.572
6.168 ± 0.788
5.381 ± 1.414
3.675 ± 1.219
8.005 ± 2.578
3.018 ± 0.657
3.806 ± 0.659
6.168 ± 0.115
8.005 ± 1.682
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.706 ± 0.375
3.543 ± 0.329
6.955 ± 0.688
4.331 ± 0.242
8.793 ± 2.202
7.087 ± 1.972
6.43 ± 0.97
3.15 ± 0.692
3.937 ± 1.306
3.018 ± 0.388
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here