Enterococcus hermanniensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2550 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L8TFW7|A0A1L8TFW7_9ENTE Uncharacterized protein OS=Enterococcus hermanniensis OX=249189 GN=RV04_GL000753 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.41KK3 pKa = 10.2VVLVLLFSSFLIAVAGCSTTNEE25 pKa = 4.18AEE27 pKa = 4.32TEE29 pKa = 4.19EE30 pKa = 4.65SSSSYY35 pKa = 11.17NIDD38 pKa = 4.51DD39 pKa = 3.93EE40 pKa = 4.94FEE42 pKa = 3.86EE43 pKa = 5.12AKK45 pKa = 10.46QQVASEE51 pKa = 4.1EE52 pKa = 4.28AYY54 pKa = 10.05EE55 pKa = 4.27PEE57 pKa = 4.3SEE59 pKa = 4.51DD60 pKa = 3.42VSEE63 pKa = 4.75SSSSSKK69 pKa = 10.43VSASIGQSQPFTSEE83 pKa = 3.77SGDD86 pKa = 3.71MIDD89 pKa = 3.97VKK91 pKa = 10.52IDD93 pKa = 3.62SVEE96 pKa = 4.07KK97 pKa = 11.16DD98 pKa = 3.38MGDD101 pKa = 3.02GDD103 pKa = 4.49YY104 pKa = 11.03YY105 pKa = 11.37VPEE108 pKa = 4.03NGYY111 pKa = 7.93YY112 pKa = 10.67AKK114 pKa = 11.11VNFTVTNLGDD124 pKa = 3.54NPFEE128 pKa = 4.39VTAHH132 pKa = 6.05QLDD135 pKa = 5.01FYY137 pKa = 11.56DD138 pKa = 4.42GDD140 pKa = 3.71NMKK143 pKa = 10.94AEE145 pKa = 4.31LNSRR149 pKa = 11.84EE150 pKa = 4.29FYY152 pKa = 11.0SDD154 pKa = 3.82TIQPGKK160 pKa = 9.57SANGVAYY167 pKa = 10.27FDD169 pKa = 4.46VKK171 pKa = 11.17NDD173 pKa = 3.43TPDD176 pKa = 3.5YY177 pKa = 10.65EE178 pKa = 5.44VYY180 pKa = 10.67FSDD183 pKa = 4.79ASWTGSYY190 pKa = 10.67

Molecular weight: 20.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L8TL75|A0A1L8TL75_9ENTE Uncharacterized protein OS=Enterococcus hermanniensis OX=249189 GN=RV04_GL000476 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.76VLAAA44 pKa = 4.31

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2550

0

2550

752147

37

2126

295.0

33.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.432 ± 0.054

0.588 ± 0.013

5.398 ± 0.038

7.214 ± 0.054

4.558 ± 0.04

6.603 ± 0.043

1.816 ± 0.021

7.691 ± 0.047

6.885 ± 0.042

9.861 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.699 ± 0.028

4.611 ± 0.035

3.416 ± 0.028

4.298 ± 0.043

3.803 ± 0.037

5.886 ± 0.036

5.941 ± 0.038

6.778 ± 0.035

0.895 ± 0.017

3.626 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski