Pectobacterium phage PP74

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Berlinvirus; Pectobacterium virus PP74

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0MF95|A0A1J0MF95_9CAUD Uncharacterized protein OS=Pectobacterium phage PP74 OX=1916101 GN=PP74_13 PE=4 SV=1
MM1 pKa = 7.43EE2 pKa = 5.74RR3 pKa = 11.84NNNAYY8 pKa = 9.97YY9 pKa = 10.74DD10 pKa = 3.97LVAATVEE17 pKa = 4.16AFNEE21 pKa = 4.41RR22 pKa = 11.84IQADD26 pKa = 4.48KK27 pKa = 10.06LTQDD31 pKa = 3.68DD32 pKa = 4.29DD33 pKa = 5.56GYY35 pKa = 11.23DD36 pKa = 3.51ALHH39 pKa = 6.2EE40 pKa = 4.31VVDD43 pKa = 4.23NNVPHH48 pKa = 6.75YY49 pKa = 8.54YY50 pKa = 10.33HH51 pKa = 7.53EE52 pKa = 4.77IFTVMAADD60 pKa = 5.5GIDD63 pKa = 3.34HH64 pKa = 6.91AFEE67 pKa = 5.61DD68 pKa = 4.42SGLMPDD74 pKa = 3.77TNDD77 pKa = 3.01VTVILQARR85 pKa = 11.84IYY87 pKa = 8.84EE88 pKa = 4.18ALWNDD93 pKa = 3.36VLNDD97 pKa = 4.14CGLVWYY103 pKa = 9.55EE104 pKa = 4.84KK105 pKa = 10.53EE106 pKa = 4.19DD107 pKa = 3.82EE108 pKa = 4.14EE109 pKa = 6.07DD110 pKa = 4.18EE111 pKa = 4.13EE112 pKa = 6.22DD113 pKa = 3.71EE114 pKa = 4.52AA115 pKa = 6.49

Molecular weight: 13.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0MER6|A0A1J0MER6_9CAUD DNA-directed DNA polymerase OS=Pectobacterium phage PP74 OX=1916101 GN=PP74_24 PE=3 SV=1
MM1 pKa = 7.03LTALFVITMYY11 pKa = 11.5ALIGAYY17 pKa = 8.92FLRR20 pKa = 11.84DD21 pKa = 4.16FKK23 pKa = 11.04RR24 pKa = 11.84GLVIHH29 pKa = 7.0KK30 pKa = 8.15ATLSHH35 pKa = 6.86FKK37 pKa = 10.87YY38 pKa = 10.57GFIPRR43 pKa = 11.84FTVRR47 pKa = 11.84EE48 pKa = 3.73RR49 pKa = 11.84NGRR52 pKa = 11.84FKK54 pKa = 11.15ANKK57 pKa = 9.12VGVFYY62 pKa = 11.11VATHH66 pKa = 6.31

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12099

26

1502

242.0

26.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.397 ± 0.667

0.984 ± 0.197

6.331 ± 0.278

7.1 ± 0.343

3.554 ± 0.211

7.711 ± 0.367

1.876 ± 0.178

4.967 ± 0.307

6.794 ± 0.48

7.935 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.19 ± 0.219

4.562 ± 0.265

3.537 ± 0.217

3.736 ± 0.237

5.356 ± 0.159

5.967 ± 0.356

5.794 ± 0.308

6.339 ± 0.353

1.513 ± 0.194

3.356 ± 0.173
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski