Pontimonas salivibrio
Average proteome isoelectric point is 5.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1693 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L2BQ19|A0A2L2BQ19_9MICO Cation-translocating P-type ATPase MgtA OS=Pontimonas salivibrio OX=1159327 GN=C3B54_11721 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.39 KK3 pKa = 9.72 ILSLVAISALALTGITALEE22 pKa = 4.15 QEE24 pKa = 4.86 AGAAADD30 pKa = 3.94 PASLSLTCTSNTTLVSSLSDD50 pKa = 3.64 EE51 pKa = 4.82 FPAGAPYY58 pKa = 10.3 VISALIGDD66 pKa = 5.36 DD67 pKa = 3.35 IDD69 pKa = 3.92 VTASGTSTDD78 pKa = 4.49 DD79 pKa = 3.71 DD80 pKa = 4.4 CDD82 pKa = 3.6 FANVSGNAALTITGGGTVEE101 pKa = 4.97 GGTSTAANEE110 pKa = 3.89 LDD112 pKa = 3.92 VANSGAFTITPVTGDD127 pKa = 3.3 AVTVYY132 pKa = 10.69 VDD134 pKa = 3.31 ACSLSGSGITSDD146 pKa = 2.96 PWLVGTAADD155 pKa = 4.32 FKK157 pKa = 11.33 SVGAEE162 pKa = 3.63 SSTAGEE168 pKa = 4.31 NSCSLAGHH176 pKa = 6.12 YY177 pKa = 9.95 LQTASLTGVDD187 pKa = 3.72 TSSVGDD193 pKa = 3.73 SDD195 pKa = 4.36 WIVDD199 pKa = 3.87 GVFTGTYY206 pKa = 10.4 DD207 pKa = 3.52 GDD209 pKa = 4.06 HH210 pKa = 6.48 YY211 pKa = 11.18 SISYY215 pKa = 9.96 FSGGGSATFQGRR227 pKa = 11.84 SPVFDD232 pKa = 3.69 EE233 pKa = 4.96 LGSGGVIKK241 pKa = 10.66 RR242 pKa = 11.84 LNLTGHH248 pKa = 6.83 IKK250 pKa = 10.77 GDD252 pKa = 3.6 STRR255 pKa = 11.84 NASLVEE261 pKa = 3.92 YY262 pKa = 10.18 LVGGTISEE270 pKa = 4.34 VRR272 pKa = 11.84 SSVWLEE278 pKa = 4.04 VEE280 pKa = 5.02 DD281 pKa = 4.28 SNALIGGLAAVTGSSSVVSDD301 pKa = 3.63 ASSRR305 pKa = 11.84 IQYY308 pKa = 9.88 SKK310 pKa = 10.84 YY311 pKa = 9.73 SGRR314 pKa = 11.84 IDD316 pKa = 3.23 WVDD319 pKa = 3.68 DD320 pKa = 3.51 GTGDD324 pKa = 4.29 VEE326 pKa = 4.63 GPTIGGLVGMVVGTGTTEE344 pKa = 4.01 LRR346 pKa = 11.84 DD347 pKa = 3.96 SYY349 pKa = 11.64 SLASISYY356 pKa = 10.54 DD357 pKa = 3.52 SGDD360 pKa = 3.69 LTGTDD365 pKa = 4.25 PDD367 pKa = 3.85 TAIYY371 pKa = 10.52 AGGLVGSDD379 pKa = 3.76 GEE381 pKa = 4.27 TDD383 pKa = 3.42 LAAFNTDD390 pKa = 3.17 DD391 pKa = 4.07 GDD393 pKa = 3.93 RR394 pKa = 11.84 TFSASEE400 pKa = 3.69 VRR402 pKa = 11.84 IVRR405 pKa = 11.84 SYY407 pKa = 10.91 FAGSFSNLCEE417 pKa = 4.69 GSAADD422 pKa = 4.9 CNTDD426 pKa = 3.3 SPSHH430 pKa = 5.96 VFTGGLFGVSEE441 pKa = 4.93 DD442 pKa = 5.43 LDD444 pKa = 4.3 DD445 pKa = 6.25 ADD447 pKa = 5.3 DD448 pKa = 4.75 LLVSAFWLSSSASNAVGEE466 pKa = 4.34 IVDD469 pKa = 5.33 AGTQPLDD476 pKa = 3.44 YY477 pKa = 10.17 TAATPALPEE486 pKa = 4.25 APGLSATLLKK496 pKa = 9.91 TVSTFQSEE504 pKa = 4.48 EE505 pKa = 4.05 GSTTGSPSGDD515 pKa = 3.3 ADD517 pKa = 4.18 LLVASSTGTLAEE529 pKa = 3.74 QDD531 pKa = 3.84 YY532 pKa = 10.55 RR533 pKa = 11.84 WAIEE537 pKa = 3.98 SGNIQTFVPSDD548 pKa = 3.58 YY549 pKa = 10.62 TSEE552 pKa = 4.26 ANYY555 pKa = 8.62 LTRR558 pKa = 11.84 QLFSDD563 pKa = 4.0 TSVSQSYY570 pKa = 9.7 RR571 pKa = 11.84 RR572 pKa = 11.84 EE573 pKa = 3.95 GAGDD577 pKa = 3.73 LTVHH581 pKa = 6.65 GGDD584 pKa = 4.26 DD585 pKa = 3.9 PEE587 pKa = 4.75 SVTGYY592 pKa = 6.83 PTLGRR597 pKa = 11.84 VWEE600 pKa = 4.37 VCSNSNSGYY609 pKa = 9.78 PFLVWEE615 pKa = 4.8 EE616 pKa = 4.45 LDD618 pKa = 3.9 CSATGGGSDD627 pKa = 5.73 DD628 pKa = 5.64 DD629 pKa = 6.16 DD630 pKa = 6.78 DD631 pKa = 6.42 DD632 pKa = 4.81 EE633 pKa = 6.19 SSEE636 pKa = 4.16 SSAAALGLTEE646 pKa = 4.6 AEE648 pKa = 4.12 YY649 pKa = 10.95 LAFLASGLTLEE660 pKa = 4.28 QFKK663 pKa = 10.47 AARR666 pKa = 11.84 LAATGPSNEE675 pKa = 3.7 TMALGFYY682 pKa = 9.77 STVLLVGMGLVLLWGYY698 pKa = 10.11 RR699 pKa = 11.84 RR700 pKa = 11.84 QRR702 pKa = 11.84 AEE704 pKa = 3.66 SSS706 pKa = 3.12
Molecular weight: 72.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.681
IPC_protein 3.719
Toseland 3.49
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.075
Thurlkill 3.541
EMBOSS 3.656
Sillero 3.846
Patrickios 1.036
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.757
Protein with the highest isoelectric point:
>tr|A0A2L2BN97|A0A2L2BN97_9MICO Foldase YidC OS=Pontimonas salivibrio OX=1159327 GN=C3B54_11139 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.5 VHH17 pKa = 5.46 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILTARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.65 GRR40 pKa = 11.84 SEE42 pKa = 3.94 LSAA45 pKa = 4.73
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.453
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.34
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.735
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.076
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1693
0
1693
538662
25
1521
318.2
34.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.106 ± 0.071
0.487 ± 0.014
5.987 ± 0.055
6.279 ± 0.05
3.31 ± 0.036
8.693 ± 0.057
2.303 ± 0.033
4.933 ± 0.04
2.653 ± 0.046
10.166 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.019 ± 0.022
2.295 ± 0.03
5.203 ± 0.04
3.464 ± 0.035
6.285 ± 0.054
6.517 ± 0.046
5.865 ± 0.047
8.948 ± 0.056
1.471 ± 0.028
2.015 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here