Salmonella virus VSe103
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385EE42|A0A385EE42_9CAUD Uncharacterized protein OS=Salmonella virus VSe103 OX=2301720 GN=vse103_26 PE=4 SV=1
MM1 pKa = 7.42 VDD3 pKa = 3.56 VIKK6 pKa = 10.74 RR7 pKa = 11.84 RR8 pKa = 11.84 IVGVSDD14 pKa = 5.64 DD15 pKa = 4.46 SPQDD19 pKa = 3.58 GQVEE23 pKa = 4.03 IDD25 pKa = 4.07 MEE27 pKa = 4.4 NVMPLRR33 pKa = 11.84 FSTGLDD39 pKa = 3.07 DD40 pKa = 3.96 TTAVTAGQAITLTVVLADD58 pKa = 3.62 GMDD61 pKa = 3.72 PKK63 pKa = 10.39 TVQWYY68 pKa = 10.33 KK69 pKa = 11.3 DD70 pKa = 3.33 NNAIAGATALTYY82 pKa = 10.32 TKK84 pKa = 10.47 ANSAATDD91 pKa = 3.16 SGTYY95 pKa = 9.83 KK96 pKa = 10.54 VVAHH100 pKa = 7.07 DD101 pKa = 4.42 GYY103 pKa = 11.85 GNIISDD109 pKa = 3.91 STVVTVSS116 pKa = 2.88
Molecular weight: 12.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.334
IPC2_protein 4.164
IPC_protein 4.126
Toseland 3.884
ProMoST 4.317
Dawson 4.139
Bjellqvist 4.291
Wikipedia 4.126
Rodwell 3.948
Grimsley 3.795
Solomon 4.126
Lehninger 4.088
Nozaki 4.266
DTASelect 4.584
Thurlkill 3.973
EMBOSS 4.126
Sillero 4.253
Patrickios 3.859
IPC_peptide 4.126
IPC2_peptide 4.228
IPC2.peptide.svr19 4.198
Protein with the highest isoelectric point:
>tr|A0A385EG61|A0A385EG61_9CAUD Uncharacterized protein OS=Salmonella virus VSe103 OX=2301720 GN=vse103_17 PE=4 SV=1
MM1 pKa = 7.25 KK2 pKa = 9.97 RR3 pKa = 11.84 APQRR7 pKa = 11.84 PSVTANAFGRR17 pKa = 11.84 AEE19 pKa = 4.5 LYY21 pKa = 10.64 CFQYY25 pKa = 10.48 LSKK28 pKa = 9.97 IRR30 pKa = 11.84 RR31 pKa = 11.84 MMFTDD36 pKa = 2.98 IRR38 pKa = 11.84 VV39 pKa = 3.09
Molecular weight: 4.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.306
IPC2_protein 9.999
IPC_protein 11.038
Toseland 10.994
ProMoST 11.067
Dawson 11.067
Bjellqvist 10.906
Wikipedia 11.389
Rodwell 11.082
Grimsley 11.125
Solomon 11.33
Lehninger 11.272
Nozaki 10.979
DTASelect 10.906
Thurlkill 10.994
EMBOSS 11.433
Sillero 11.023
Patrickios 10.906
IPC_peptide 11.33
IPC2_peptide 10.233
IPC2.peptide.svr19 8.395
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
13048
39
1032
228.9
25.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.664 ± 0.579
1.042 ± 0.138
6.101 ± 0.201
6.568 ± 0.335
3.832 ± 0.153
7.718 ± 0.299
1.64 ± 0.143
4.836 ± 0.212
5.901 ± 0.319
7.787 ± 0.291
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.583 ± 0.142
4.414 ± 0.229
3.932 ± 0.203
3.633 ± 0.241
5.717 ± 0.218
5.848 ± 0.275
6.537 ± 0.304
7.396 ± 0.311
1.433 ± 0.136
3.418 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here