Klebsiella phage ST16-OXA48phi5.4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Reipivirus; Klebsiella virus ST16OXA48phi5-4

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482ILV6|A0A482ILV6_9CAUD Ogr_Delta domain-containing protein OS=Klebsiella phage ST16-OXA48phi5.4 OX=2510483 PE=4 SV=1
MM1 pKa = 7.19KK2 pKa = 9.9TFNQLKK8 pKa = 10.35SLIDD12 pKa = 3.78FCQTDD17 pKa = 3.56AFFLEE22 pKa = 4.47HH23 pKa = 7.11LNRR26 pKa = 11.84LQIAGVIYY34 pKa = 10.55LDD36 pKa = 4.56EE37 pKa = 6.47GDD39 pKa = 4.06IDD41 pKa = 4.98ADD43 pKa = 3.74HH44 pKa = 6.58KK45 pKa = 9.9TVSDD49 pKa = 3.85DD50 pKa = 3.91FYY52 pKa = 11.77DD53 pKa = 3.88RR54 pKa = 11.84LASVYY59 pKa = 10.61GIEE62 pKa = 4.26PEE64 pKa = 4.24IKK66 pKa = 10.25SEE68 pKa = 4.02EE69 pKa = 4.0AA70 pKa = 3.3

Molecular weight: 8.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482IL36|A0A482IL36_9CAUD Probable tape measure protein OS=Klebsiella phage ST16-OXA48phi5.4 OX=2510483 PE=3 SV=1
MM1 pKa = 7.67SDD3 pKa = 3.32RR4 pKa = 11.84MFSEE8 pKa = 4.91LDD10 pKa = 3.27QVFQDD15 pKa = 3.68ILDD18 pKa = 4.23GVSPAGRR25 pKa = 11.84TRR27 pKa = 11.84TARR30 pKa = 11.84KK31 pKa = 9.1IGLALRR37 pKa = 11.84RR38 pKa = 11.84SQQRR42 pKa = 11.84RR43 pKa = 11.84IASQKK48 pKa = 9.76NPDD51 pKa = 3.18GSGYY55 pKa = 10.25AVRR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84KK61 pKa = 9.83VYY63 pKa = 9.1RR64 pKa = 11.84TQQGIKK70 pKa = 9.86FFWNNEE76 pKa = 3.18VRR78 pKa = 11.84ALKK81 pKa = 10.04NWRR84 pKa = 11.84GGRR87 pKa = 11.84GKK89 pKa = 10.48YY90 pKa = 10.19GRR92 pKa = 11.84TITGFDD98 pKa = 3.25EE99 pKa = 3.93KK100 pKa = 10.85RR101 pKa = 11.84RR102 pKa = 11.84DD103 pKa = 2.78IRR105 pKa = 11.84TFYY108 pKa = 10.59RR109 pKa = 11.84ADD111 pKa = 3.04IEE113 pKa = 4.39RR114 pKa = 11.84YY115 pKa = 10.17LEE117 pKa = 4.16IKK119 pKa = 8.14TQSATHH125 pKa = 6.23SEE127 pKa = 4.35TKK129 pKa = 9.14KK130 pKa = 10.79APMFTRR136 pKa = 11.84LRR138 pKa = 11.84TLRR141 pKa = 11.84FMKK144 pKa = 10.32LRR146 pKa = 11.84PDD148 pKa = 3.42AGGVTVGFDD157 pKa = 3.89GIAARR162 pKa = 11.84IARR165 pKa = 11.84IHH167 pKa = 6.08QYY169 pKa = 10.06GLKK172 pKa = 10.73DD173 pKa = 3.6EE174 pKa = 4.84VGPGAYY180 pKa = 9.33AQYY183 pKa = 9.91PARR186 pKa = 11.84EE187 pKa = 4.16LLGITPADD195 pKa = 3.73LSATEE200 pKa = 4.0NAVISSLGGASS211 pKa = 3.08

Molecular weight: 23.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

11656

38

1008

253.4

28.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.411 ± 0.505

1.295 ± 0.214

5.8 ± 0.307

6.083 ± 0.312

4.127 ± 0.272

7.104 ± 0.5

1.802 ± 0.167

5.654 ± 0.323

5.225 ± 0.352

9.394 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.076 ± 0.111

4.367 ± 0.364

3.723 ± 0.188

3.809 ± 0.292

6.28 ± 0.413

7.018 ± 0.333

6.443 ± 0.317

6.031 ± 0.279

1.381 ± 0.15

2.977 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski