Methanomassiliicoccaceae archaeon DOK

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Methanomassiliicoccales; Methanomassiliicoccaceae; unclassified Methanomassiliicoccaceae

Average proteome isoelectric point is 5.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1835 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9TCW7|A0A6B9TCW7_9ARCH Flavodoxin OS=Methanomassiliicoccaceae archaeon DOK OX=1535962 GN=JS82_08600 PE=4 SV=1
MM1 pKa = 7.56NSITKK6 pKa = 10.36AIALLVVLLTATAGVAMMADD26 pKa = 3.59SSDD29 pKa = 3.56AAPADD34 pKa = 3.32IQEE37 pKa = 4.83PVPASNPEE45 pKa = 3.91AGTAIAKK52 pKa = 9.99VDD54 pKa = 3.54GTPYY58 pKa = 10.5TDD60 pKa = 4.33FEE62 pKa = 4.83VAVDD66 pKa = 3.91AAISSNKK73 pKa = 8.73TLTLLNDD80 pKa = 3.66YY81 pKa = 10.88QITNLNLDD89 pKa = 4.16AEE91 pKa = 4.74SVTIDD96 pKa = 4.35LNTKK100 pKa = 8.82ILSFEE105 pKa = 4.31RR106 pKa = 11.84IKK108 pKa = 10.96LINASSLTFEE118 pKa = 4.56NGTLNGLYY126 pKa = 9.97IGAANEE132 pKa = 3.54ASIAVNTGCSVTLNDD147 pKa = 3.04VNYY150 pKa = 7.86YY151 pKa = 9.56TKK153 pKa = 10.35ATGLFPSGDD162 pKa = 3.61AASVTVKK169 pKa = 10.65NGSVVHH175 pKa = 6.61AVGFCIGTNAGSSSNYY191 pKa = 9.64YY192 pKa = 10.43VDD194 pKa = 4.63IKK196 pKa = 10.88ISDD199 pKa = 3.52SGIYY203 pKa = 10.13ADD205 pKa = 4.78SDD207 pKa = 3.64SSAAGTAVFYY217 pKa = 10.33NVPGSLTITDD227 pKa = 3.95STVQGYY233 pKa = 7.01FHH235 pKa = 6.74GVIVRR240 pKa = 11.84GGTAVISGSTITNTMNLSGWEE261 pKa = 4.08TYY263 pKa = 10.59YY264 pKa = 11.09DD265 pKa = 3.83SEE267 pKa = 4.19PWGTGNSIKK276 pKa = 10.7LGALVLGNRR285 pKa = 11.84DD286 pKa = 3.44NASYY290 pKa = 11.03NYY292 pKa = 7.54PTDD295 pKa = 3.19VTLIDD300 pKa = 3.82TDD302 pKa = 4.18VISDD306 pKa = 3.63GTYY309 pKa = 8.67GANYY313 pKa = 8.95SAVYY317 pKa = 8.78MWANPDD323 pKa = 3.56PEE325 pKa = 5.03NGVNLVYY332 pKa = 10.88DD333 pKa = 4.14SACTFSNLNSTTNDD347 pKa = 2.79LFFYY351 pKa = 10.44GNNGANITAGLAVAQAGDD369 pKa = 4.06RR370 pKa = 11.84IYY372 pKa = 11.46NSLQAAIDD380 pKa = 4.12DD381 pKa = 4.35DD382 pKa = 4.48NEE384 pKa = 4.33EE385 pKa = 4.01ITLVGDD391 pKa = 3.42TKK393 pKa = 11.34EE394 pKa = 4.07NVVVDD399 pKa = 4.52GQTVTLDD406 pKa = 3.77LNGKK410 pKa = 6.77TVTNVDD416 pKa = 3.23DD417 pKa = 3.5HH418 pKa = 6.77TITVKK423 pKa = 10.67SGEE426 pKa = 4.21LTVKK430 pKa = 10.76GKK432 pKa = 8.76GTVQNLTHH440 pKa = 6.32QKK442 pKa = 10.27AALAAYY448 pKa = 7.92PGTKK452 pKa = 8.63VTLNGGTFDD461 pKa = 3.78RR462 pKa = 11.84TNEE465 pKa = 4.15TGTGPDD471 pKa = 3.12TSGNNSWYY479 pKa = 9.26TIKK482 pKa = 10.72NHH484 pKa = 6.2GDD486 pKa = 3.24MTIDD490 pKa = 3.39GATVRR495 pKa = 11.84NGGGFSSCINNGWYY509 pKa = 10.05SGSAQGTPGNNDD521 pKa = 3.29LAVYY525 pKa = 10.48AGTNAKK531 pKa = 8.59LTIVSGKK538 pKa = 9.79IDD540 pKa = 3.59GGLNAVKK547 pKa = 10.5NDD549 pKa = 3.86DD550 pKa = 3.85YY551 pKa = 12.05GEE553 pKa = 4.42LLVQGGEE560 pKa = 4.33LTNSVQATILNWNIATIEE578 pKa = 4.16KK579 pKa = 9.02GTFSATSDD587 pKa = 3.24AKK589 pKa = 11.01AVILNGKK596 pKa = 9.18IDD598 pKa = 3.43GTMDD602 pKa = 3.28KK603 pKa = 11.03GQLTISDD610 pKa = 4.06GTFSGPVGIQMMSGGVGIGDD630 pKa = 3.48VTITGGTFNISSDD643 pKa = 4.04NIIDD647 pKa = 4.22PLTGTEE653 pKa = 4.17TNVQVSGGSFSDD665 pKa = 4.12DD666 pKa = 2.96SVLAYY671 pKa = 10.43LVEE674 pKa = 4.46GKK676 pKa = 10.84GLVEE680 pKa = 4.5NGTMFDD686 pKa = 3.52VVDD689 pKa = 3.8VHH691 pKa = 5.86TVTYY695 pKa = 9.09TFNDD699 pKa = 3.14TTYY702 pKa = 9.8IEE704 pKa = 4.53KK705 pKa = 10.73VISGQTATQNTIPALPTLPEE725 pKa = 4.01GCEE728 pKa = 3.79YY729 pKa = 11.26DD730 pKa = 3.44FGEE733 pKa = 4.19WDD735 pKa = 3.08KK736 pKa = 10.37TAPVTEE742 pKa = 4.42DD743 pKa = 2.98VSVSVKK749 pKa = 11.1AEE751 pKa = 3.87ITSLTVAIGTSTVDD765 pKa = 3.34GVTTLTATVDD775 pKa = 3.64STASGTPAYY784 pKa = 8.03TWSSGEE790 pKa = 3.95ATEE793 pKa = 5.49SITPSVGGTYY803 pKa = 10.62DD804 pKa = 3.25VTVTLTVTSGDD815 pKa = 3.44VTVTGKK821 pKa = 10.8ANASVTYY828 pKa = 9.02TPSEE832 pKa = 4.32SGSTTTTTTNPDD844 pKa = 2.85GSTTTVEE851 pKa = 4.1TDD853 pKa = 2.56SDD855 pKa = 4.23GSVTEE860 pKa = 4.45TTTKK864 pKa = 10.63TEE866 pKa = 3.93TTEE869 pKa = 3.96TGNEE873 pKa = 3.66ITTVTTTGSDD883 pKa = 3.36PSGSQTTSSTEE894 pKa = 3.81VTIEE898 pKa = 3.99AASGTSGSVSLPEE911 pKa = 3.92EE912 pKa = 4.36DD913 pKa = 3.6VNSVIEE919 pKa = 4.56SIASAGTTTNTISVNIGEE937 pKa = 4.66NDD939 pKa = 3.65TVTMGSAAISAIAEE953 pKa = 4.24SNAVLEE959 pKa = 4.38ISNEE963 pKa = 3.94NATVVADD970 pKa = 3.66GDD972 pKa = 4.19VIEE975 pKa = 4.64TLSTGDD981 pKa = 3.13VSITVGSATHH991 pKa = 7.22DD992 pKa = 3.74NLNEE996 pKa = 4.1SQQQSVPEE1004 pKa = 3.81NSTIVEE1010 pKa = 4.23LSATVGTSPVHH1021 pKa = 6.39DD1022 pKa = 4.39LNGTVEE1028 pKa = 4.09VTMTYY1033 pKa = 9.25TLPSGIDD1040 pKa = 3.35ADD1042 pKa = 4.2DD1043 pKa = 4.62VIVFYY1048 pKa = 11.37VDD1050 pKa = 4.06DD1051 pKa = 5.87DD1052 pKa = 4.5GALHH1056 pKa = 6.49QMRR1059 pKa = 11.84TVYY1062 pKa = 10.91AEE1064 pKa = 4.86GILTFYY1070 pKa = 7.38TTHH1073 pKa = 6.24FSYY1076 pKa = 11.22YY1077 pKa = 9.83FVGDD1081 pKa = 3.17RR1082 pKa = 11.84SMIPSVDD1089 pKa = 3.84PEE1091 pKa = 4.26PSPGEE1096 pKa = 3.9DD1097 pKa = 3.45DD1098 pKa = 3.78QPVVVPPVDD1107 pKa = 5.25DD1108 pKa = 5.45DD1109 pKa = 4.75DD1110 pKa = 7.13DD1111 pKa = 4.39YY1112 pKa = 12.26VPLPPIVDD1120 pKa = 3.66TDD1122 pKa = 3.89GSGSSGGDD1130 pKa = 3.11DD1131 pKa = 3.19TVKK1134 pKa = 10.49IVACAAAAVVAALMAAFLIISRR1156 pKa = 11.84RR1157 pKa = 11.84DD1158 pKa = 3.09

Molecular weight: 119.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9T8U7|A0A6B9T8U7_9ARCH Abortive phage infection protein OS=Methanomassiliicoccaceae archaeon DOK OX=1535962 GN=JS82_00450 PE=4 SV=1
MM1 pKa = 7.67SSTKK5 pKa = 10.4APAMKK10 pKa = 9.91QRR12 pKa = 11.84LNKK15 pKa = 9.84KK16 pKa = 8.11VNQNRR21 pKa = 11.84RR22 pKa = 11.84VPAWVMLRR30 pKa = 11.84TNRR33 pKa = 11.84QFLRR37 pKa = 11.84HH38 pKa = 5.85PKK40 pKa = 8.59RR41 pKa = 11.84RR42 pKa = 11.84SWRR45 pKa = 11.84MSKK48 pKa = 10.58LKK50 pKa = 10.36EE51 pKa = 3.79

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1835

0

1835

554497

27

3418

302.2

33.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.112 ± 0.066

1.607 ± 0.029

7.116 ± 0.052

6.834 ± 0.062

3.592 ± 0.038

8.296 ± 0.065

1.636 ± 0.021

6.402 ± 0.05

4.328 ± 0.06

8.031 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.498 ± 0.038

3.303 ± 0.055

4.029 ± 0.041

2.278 ± 0.029

5.948 ± 0.091

6.531 ± 0.058

5.713 ± 0.095

8.365 ± 0.059

0.964 ± 0.021

3.416 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski