Prevotella sp. CAG:873

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2055 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7J5D1|R7J5D1_9BACT Ser/Thr protein phosphatase family protein OS=Prevotella sp. CAG:873 OX=1262936 GN=BN799_00188 PE=4 SV=1
MM1 pKa = 7.67PLSNNTLAVMALCNEE16 pKa = 4.26YY17 pKa = 10.41CQAIQSVASGQLAGDD32 pKa = 4.79DD33 pKa = 3.84DD34 pKa = 4.23TSARR38 pKa = 11.84DD39 pKa = 3.73TFVSTMLRR47 pKa = 11.84LLPRR51 pKa = 11.84IYY53 pKa = 10.14IAANDD58 pKa = 3.79IPEE61 pKa = 4.49SLDD64 pKa = 3.31DD65 pKa = 3.64TDD67 pKa = 5.95AYY69 pKa = 9.94IADD72 pKa = 3.91VLDD75 pKa = 3.96EE76 pKa = 4.93DD77 pKa = 4.37SYY79 pKa = 11.47EE80 pKa = 4.16ALRR83 pKa = 11.84SAIEE87 pKa = 3.95QLLGADD93 pKa = 3.64DD94 pKa = 4.32TYY96 pKa = 11.9LEE98 pKa = 4.72VFHH101 pKa = 7.98QDD103 pKa = 2.66MKK105 pKa = 11.55YY106 pKa = 10.81SDD108 pKa = 3.84TPIAASIAEE117 pKa = 4.3GLCDD121 pKa = 4.28IFQSTYY127 pKa = 11.24NYY129 pKa = 10.28LQTIRR134 pKa = 11.84DD135 pKa = 3.92DD136 pKa = 3.53ATDD139 pKa = 3.63RR140 pKa = 11.84VVDD143 pKa = 3.72AATEE147 pKa = 3.85ALRR150 pKa = 11.84ADD152 pKa = 5.6FIQYY156 pKa = 9.3WSGILCNVLRR166 pKa = 11.84ALNALRR172 pKa = 11.84ACC174 pKa = 4.53

Molecular weight: 19.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7IZL4|R7IZL4_9BACT 5'-Nucleotidase domain protein OS=Prevotella sp. CAG:873 OX=1262936 GN=BN799_01098 PE=3 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.36KK6 pKa = 9.96RR7 pKa = 11.84KK8 pKa = 6.44GHH10 pKa = 6.2KK11 pKa = 9.14MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.26NRR25 pKa = 11.84HH26 pKa = 4.71KK27 pKa = 10.87KK28 pKa = 9.36KK29 pKa = 10.77

Molecular weight: 3.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2055

0

2055

701839

29

2498

341.5

37.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.299 ± 0.073

1.355 ± 0.021

6.37 ± 0.04

5.301 ± 0.052

3.822 ± 0.037

7.112 ± 0.051

2.122 ± 0.027

6.754 ± 0.043

4.857 ± 0.053

8.397 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.024

4.419 ± 0.046

4.109 ± 0.027

3.028 ± 0.03

5.893 ± 0.057

6.123 ± 0.048

6.234 ± 0.045

6.91 ± 0.043

1.142 ± 0.02

4.089 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski