Amino acid dipepetide frequency for Prevotella sp. CAG:873

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.106AlaAla: 10.106 ± 0.182	1.081AlaCys: 1.081 ± 0.045	6.122AlaAsp: 6.122 ± 0.09	5.239AlaGlu: 5.239 ± 0.114	3.391AlaPhe: 3.391 ± 0.077	6.047AlaGly: 6.047 ± 0.114	1.731AlaHis: 1.731 ± 0.05	6.145AlaIle: 6.145 ± 0.12	4.25AlaLys: 4.25 ± 0.094	8.602AlaLeu: 8.602 ± 0.134	2.684AlaMet: 2.684 ± 0.073	3.519AlaAsn: 3.519 ± 0.083	3.576AlaPro: 3.576 ± 0.085	3.273AlaGln: 3.273 ± 0.073	5.034AlaArg: 5.034 ± 0.097	5.35AlaSer: 5.35 ± 0.092	5.63AlaThr: 5.63 ± 0.104	7.044AlaVal: 7.044 ± 0.123	0.955AlaTrp: 0.955 ± 0.039	3.207AlaTyr: 3.207 ± 0.072	0.006AlaXaa: 0.006 ± 0.003
Cys	1.207CysAla: 1.207 ± 0.047	0.199CysCys: 0.199 ± 0.017	0.851CysAsp: 0.851 ± 0.04	0.594CysGlu: 0.594 ± 0.032	0.499CysPhe: 0.499 ± 0.028	1.307CysGly: 1.307 ± 0.055	0.36CysHis: 0.36 ± 0.021	0.902CysIle: 0.902 ± 0.038	0.507CysLys: 0.507 ± 0.028	0.985CysLeu: 0.985 ± 0.043	0.311CysMet: 0.311 ± 0.018	0.56CysAsn: 0.56 ± 0.029	0.628CysPro: 0.628 ± 0.034	0.351CysGln: 0.351 ± 0.021	1.08CysArg: 1.08 ± 0.04	0.895CysSer: 0.895 ± 0.045	0.799CysThr: 0.799 ± 0.039	0.889CysVal: 0.889 ± 0.043	0.137CysTrp: 0.137 ± 0.013	0.489CysTyr: 0.489 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	6.141AspAla: 6.141 ± 0.105	0.741AspCys: 0.741 ± 0.034	3.786AspAsp: 3.786 ± 0.093	3.725AspGlu: 3.725 ± 0.092	2.82AspPhe: 2.82 ± 0.062	5.017AspGly: 5.017 ± 0.112	0.99AspHis: 0.99 ± 0.036	5.412AspIle: 5.412 ± 0.107	3.37AspLys: 3.37 ± 0.064	4.665AspLeu: 4.665 ± 0.086	1.77AspMet: 1.77 ± 0.048	3.189AspAsn: 3.189 ± 0.074	2.381AspPro: 2.381 ± 0.061	1.237AspGln: 1.237 ± 0.045	3.19AspArg: 3.19 ± 0.073	3.675AspSer: 3.675 ± 0.076	3.984AspThr: 3.984 ± 0.078	4.255AspVal: 4.255 ± 0.093	0.678AspTrp: 0.678 ± 0.033	2.676AspTyr: 2.676 ± 0.073	0.0AspXaa: 0.0 ± 0.0
Glu	5.333GluAla: 5.333 ± 0.112	0.673GluCys: 0.673 ± 0.034	3.388GluAsp: 3.388 ± 0.069	2.797GluGlu: 2.797 ± 0.08	1.842GluPhe: 1.842 ± 0.053	3.62GluGly: 3.62 ± 0.084	1.178GluHis: 1.178 ± 0.041	3.746GluIle: 3.746 ± 0.082	3.049GluLys: 3.049 ± 0.087	4.236GluLeu: 4.236 ± 0.091	1.549GluMet: 1.549 ± 0.058	2.265GluAsn: 2.265 ± 0.053	1.723GluPro: 1.723 ± 0.052	2.077GluGln: 2.077 ± 0.059	3.175GluArg: 3.175 ± 0.082	2.677GluSer: 2.677 ± 0.067	2.91GluThr: 2.91 ± 0.067	3.585GluVal: 3.585 ± 0.073	0.606GluTrp: 0.606 ± 0.027	2.578GluTyr: 2.578 ± 0.066	0.003GluXaa: 0.003 ± 0.002
Phe	3.458PheAla: 3.458 ± 0.08	0.55PheCys: 0.55 ± 0.029	2.904PheAsp: 2.904 ± 0.069	2.213PheGlu: 2.213 ± 0.059	1.667PhePhe: 1.667 ± 0.068	3.261PheGly: 3.261 ± 0.063	0.691PheHis: 0.691 ± 0.027	2.223PheIle: 2.223 ± 0.073	1.748PheLys: 1.748 ± 0.054	2.778PheLeu: 2.778 ± 0.066	0.966PheMet: 0.966 ± 0.04	1.992PheAsn: 1.992 ± 0.056	1.389PhePro: 1.389 ± 0.048	0.768PheGln: 0.768 ± 0.037	1.951PheArg: 1.951 ± 0.054	2.491PheSer: 2.491 ± 0.071	2.392PheThr: 2.392 ± 0.068	2.865PheVal: 2.865 ± 0.064	0.395PheTrp: 0.395 ± 0.024	1.513PheTyr: 1.513 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	5.758GlyAla: 5.758 ± 0.108	1.221GlyCys: 1.221 ± 0.056	4.414GlyAsp: 4.414 ± 0.088	3.609GlyGlu: 3.609 ± 0.065	2.706GlyPhe: 2.706 ± 0.061	5.018GlyGly: 5.018 ± 0.114	1.714GlyHis: 1.714 ± 0.055	5.441GlyIle: 5.441 ± 0.099	3.975GlyLys: 3.975 ± 0.084	5.699GlyLeu: 5.699 ± 0.101	1.905GlyMet: 1.905 ± 0.058	3.294GlyAsn: 3.294 ± 0.088	1.553GlyPro: 1.553 ± 0.047	2.109GlyGln: 2.109 ± 0.056	4.216GlyArg: 4.216 ± 0.089	4.176GlySer: 4.176 ± 0.095	4.84GlyThr: 4.84 ± 0.087	5.393GlyVal: 5.393 ± 0.114	0.916GlyTrp: 0.916 ± 0.032	3.157GlyTyr: 3.157 ± 0.08	0.006GlyXaa: 0.006 ± 0.003
His	1.71HisAla: 1.71 ± 0.055	0.332HisCys: 0.332 ± 0.022	1.228HisAsp: 1.228 ± 0.046	0.868HisGlu: 0.868 ± 0.038	0.875HisPhe: 0.875 ± 0.036	1.611HisGly: 1.611 ± 0.055	0.559HisHis: 0.559 ± 0.032	1.755HisIle: 1.755 ± 0.062	0.94HisLys: 0.94 ± 0.035	1.817HisLeu: 1.817 ± 0.05	0.425HisMet: 0.425 ± 0.024	1.153HisAsn: 1.153 ± 0.04	1.134HisPro: 1.134 ± 0.042	0.539HisGln: 0.539 ± 0.028	1.379HisArg: 1.379 ± 0.046	1.284HisSer: 1.284 ± 0.051	1.314HisThr: 1.314 ± 0.044	1.177HisVal: 1.177 ± 0.043	0.252HisTrp: 0.252 ± 0.018	0.865HisTyr: 0.865 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	6.537IleAla: 6.537 ± 0.106	0.995IleCys: 0.995 ± 0.04	4.99IleAsp: 4.99 ± 0.097	3.981IleGlu: 3.981 ± 0.083	2.495IlePhe: 2.495 ± 0.066	4.76IleGly: 4.76 ± 0.097	1.308IleHis: 1.308 ± 0.044	4.622IleIle: 4.622 ± 0.106	3.166IleLys: 3.166 ± 0.073	5.221IleLeu: 5.221 ± 0.093	1.384IleMet: 1.384 ± 0.05	2.999IleAsn: 2.999 ± 0.072	3.153IlePro: 3.153 ± 0.075	1.73IleGln: 1.73 ± 0.05	3.546IleArg: 3.546 ± 0.06	4.34IleSer: 4.34 ± 0.084	4.195IleThr: 4.195 ± 0.08	5.05IleVal: 5.05 ± 0.091	0.554IleTrp: 0.554 ± 0.028	2.515IleTyr: 2.515 ± 0.07	0.0IleXaa: 0.0 ± 0.0
Lys	4.675LysAla: 4.675 ± 0.093	0.536LysCys: 0.536 ± 0.029	3.358LysAsp: 3.358 ± 0.073	2.74LysGlu: 2.74 ± 0.073	1.55LysPhe: 1.55 ± 0.047	3.283LysGly: 3.283 ± 0.081	1.091LysHis: 1.091 ± 0.035	2.991LysIle: 2.991 ± 0.073	2.86LysLys: 2.86 ± 0.076	3.564LysLeu: 3.564 ± 0.077	1.634LysMet: 1.634 ± 0.048	2.117LysAsn: 2.117 ± 0.061	1.868LysPro: 1.868 ± 0.062	1.599LysGln: 1.599 ± 0.054	2.667LysArg: 2.667 ± 0.077	2.844LysSer: 2.844 ± 0.081	3.145LysThr: 3.145 ± 0.073	3.316LysVal: 3.316 ± 0.071	0.54LysTrp: 0.54 ± 0.034	2.194LysTyr: 2.194 ± 0.066	0.001LysXaa: 0.001 ± 0.001
Leu	7.8LeuAla: 7.8 ± 0.121	1.466LeuCys: 1.466 ± 0.045	5.142LeuAsp: 5.142 ± 0.095	4.195LeuGlu: 4.195 ± 0.094	2.921LeuPhe: 2.921 ± 0.076	5.88LeuGly: 5.88 ± 0.099	1.953LeuHis: 1.953 ± 0.051	4.433LeuIle: 4.433 ± 0.1	3.951LeuLys: 3.951 ± 0.091	7.069LeuLeu: 7.069 ± 0.149	2.25LeuMet: 2.25 ± 0.066	3.635LeuAsn: 3.635 ± 0.081	3.948LeuPro: 3.948 ± 0.076	2.59LeuGln: 2.59 ± 0.071	5.822LeuArg: 5.822 ± 0.095	6.058LeuSer: 6.058 ± 0.096	5.574LeuThr: 5.574 ± 0.104	5.129LeuVal: 5.129 ± 0.086	0.996LeuTrp: 0.996 ± 0.042	3.159LeuTyr: 3.159 ± 0.07	0.001LeuXaa: 0.001 ± 0.001
Met	2.734MetAla: 2.734 ± 0.068	0.318MetCys: 0.318 ± 0.02	1.536MetAsp: 1.536 ± 0.051	1.2MetGlu: 1.2 ± 0.049	0.967MetPhe: 0.967 ± 0.041	1.741MetGly: 1.741 ± 0.051	0.64MetHis: 0.64 ± 0.032	1.275MetIle: 1.275 ± 0.044	1.396MetLys: 1.396 ± 0.041	2.535MetLeu: 2.535 ± 0.068	0.69MetMet: 0.69 ± 0.033	0.956MetAsn: 0.956 ± 0.041	1.546MetPro: 1.546 ± 0.047	0.875MetGln: 0.875 ± 0.035	1.774MetArg: 1.774 ± 0.049	1.805MetSer: 1.805 ± 0.046	1.765MetThr: 1.765 ± 0.044	1.713MetVal: 1.713 ± 0.047	0.265MetTrp: 0.265 ± 0.02	0.848MetTyr: 0.848 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	4.232AsnAla: 4.232 ± 0.084	0.549AsnCys: 0.549 ± 0.033	2.66AsnAsp: 2.66 ± 0.068	2.097AsnGlu: 2.097 ± 0.064	1.818AsnPhe: 1.818 ± 0.054	3.535AsnGly: 3.535 ± 0.084	0.779AsnHis: 0.779 ± 0.034	3.488AsnIle: 3.488 ± 0.086	2.16AsnLys: 2.16 ± 0.067	3.34AsnLeu: 3.34 ± 0.076	1.11AsnMet: 1.11 ± 0.043	2.199AsnAsn: 2.199 ± 0.071	2.378AsnPro: 2.378 ± 0.064	1.032AsnGln: 1.032 ± 0.044	2.251AsnArg: 2.251 ± 0.06	2.525AsnSer: 2.525 ± 0.066	2.67AsnThr: 2.67 ± 0.062	3.092AsnVal: 3.092 ± 0.063	0.5AsnTrp: 0.5 ± 0.031	1.774AsnTyr: 1.774 ± 0.051	0.0AsnXaa: 0.0 ± 0.0
Pro	3.655ProAla: 3.655 ± 0.08	0.399ProCys: 0.399 ± 0.024	2.898ProAsp: 2.898 ± 0.066	3.304ProGlu: 3.304 ± 0.074	1.465ProPhe: 1.465 ± 0.047	2.996ProGly: 2.996 ± 0.075	0.762ProHis: 0.762 ± 0.03	2.169ProIle: 2.169 ± 0.058	1.915ProLys: 1.915 ± 0.053	3.35ProLeu: 3.35 ± 0.062	1.12ProMet: 1.12 ± 0.042	1.654ProAsn: 1.654 ± 0.054	1.143ProPro: 1.143 ± 0.049	1.582ProGln: 1.582 ± 0.049	2.067ProArg: 2.067 ± 0.061	2.603ProSer: 2.603 ± 0.072	2.509ProThr: 2.509 ± 0.07	3.19ProVal: 3.19 ± 0.071	0.514ProTrp: 0.514 ± 0.029	1.788ProTyr: 1.788 ± 0.051	0.01ProXaa: 0.01 ± 0.004
Gln	2.754GlnAla: 2.754 ± 0.076	0.398GlnCys: 0.398 ± 0.024	1.51GlnAsp: 1.51 ± 0.048	1.338GlnGlu: 1.338 ± 0.053	1.029GlnPhe: 1.029 ± 0.042	2.075GlnGly: 2.075 ± 0.066	0.637GlnHis: 0.637 ± 0.029	1.935GlnIle: 1.935 ± 0.055	1.517GlnLys: 1.517 ± 0.048	2.706GlnLeu: 2.706 ± 0.071	0.973GlnMet: 0.973 ± 0.04	1.25GlnAsn: 1.25 ± 0.048	1.25GlnPro: 1.25 ± 0.043	1.365GlnGln: 1.365 ± 0.06	2.176GlnArg: 2.176 ± 0.065	1.845GlnSer: 1.845 ± 0.054	1.691GlnThr: 1.691 ± 0.057	1.832GlnVal: 1.832 ± 0.053	0.541GlnTrp: 0.541 ± 0.027	1.456GlnTyr: 1.456 ± 0.048	0.001GlnXaa: 0.001 ± 0.001
Arg	4.568ArgAla: 4.568 ± 0.097	0.759ArgCys: 0.759 ± 0.044	3.393ArgAsp: 3.393 ± 0.08	2.788ArgGlu: 2.788 ± 0.067	2.33ArgPhe: 2.33 ± 0.058	3.395ArgGly: 3.395 ± 0.064	1.807ArgHis: 1.807 ± 0.053	4.129ArgIle: 4.129 ± 0.072	2.562ArgLys: 2.562 ± 0.072	5.904ArgLeu: 5.904 ± 0.101	1.734ArgMet: 1.734 ± 0.052	2.559ArgAsn: 2.559 ± 0.065	2.569ArgPro: 2.569 ± 0.074	2.462ArgGln: 2.462 ± 0.068	5.08ArgArg: 5.08 ± 0.124	2.847ArgSer: 2.847 ± 0.067	3.053ArgThr: 3.053 ± 0.067	3.516ArgVal: 3.516 ± 0.077	0.852ArgTrp: 0.852 ± 0.043	2.617ArgTyr: 2.617 ± 0.066	0.003ArgXaa: 0.003 ± 0.002
Ser	5.525SerAla: 5.525 ± 0.106	0.777SerCys: 0.777 ± 0.033	3.642SerAsp: 3.642 ± 0.076	3.243SerGlu: 3.243 ± 0.066	2.478SerPhe: 2.478 ± 0.069	4.739SerGly: 4.739 ± 0.097	1.274SerHis: 1.274 ± 0.041	4.178SerIle: 4.178 ± 0.087	2.961SerLys: 2.961 ± 0.065	5.527SerLeu: 5.527 ± 0.1	1.603SerMet: 1.603 ± 0.051	2.55SerAsn: 2.55 ± 0.073	2.419SerPro: 2.419 ± 0.063	1.848SerGln: 1.848 ± 0.053	3.353SerArg: 3.353 ± 0.069	3.769SerSer: 3.769 ± 0.101	3.742SerThr: 3.742 ± 0.081	4.501SerVal: 4.501 ± 0.106	0.708SerTrp: 0.708 ± 0.035	2.389SerTyr: 2.389 ± 0.069	0.001SerXaa: 0.001 ± 0.001
Thr	6.063ThrAla: 6.063 ± 0.119	0.658ThrCys: 0.658 ± 0.031	3.881ThrAsp: 3.881 ± 0.087	2.988ThrGlu: 2.988 ± 0.07	2.586ThrPhe: 2.586 ± 0.055	4.487ThrGly: 4.487 ± 0.095	1.247ThrHis: 1.247 ± 0.045	4.762ThrIle: 4.762 ± 0.092	2.471ThrLys: 2.471 ± 0.064	5.979ThrLeu: 5.979 ± 0.094	1.475ThrMet: 1.475 ± 0.048	2.275ThrAsn: 2.275 ± 0.062	3.511ThrPro: 3.511 ± 0.077	1.676ThrGln: 1.676 ± 0.052	3.075ThrArg: 3.075 ± 0.068	3.886ThrSer: 3.886 ± 0.112	3.935ThrThr: 3.935 ± 0.091	4.236ThrVal: 4.236 ± 0.09	0.621ThrTrp: 0.621 ± 0.027	2.528ThrTyr: 2.528 ± 0.067	0.001ThrXaa: 0.001 ± 0.001
Val	6.366ValAla: 6.366 ± 0.119	1.097ValCys: 1.097 ± 0.039	4.347ValAsp: 4.347 ± 0.077	3.562ValGlu: 3.562 ± 0.081	2.701ValPhe: 2.701 ± 0.067	4.528ValGly: 4.528 ± 0.091	1.335ValHis: 1.335 ± 0.049	4.463ValIle: 4.463 ± 0.091	3.401ValLys: 3.401 ± 0.072	5.715ValLeu: 5.715 ± 0.09	1.674ValMet: 1.674 ± 0.051	3.116ValAsn: 3.116 ± 0.082	3.014ValPro: 3.014 ± 0.066	1.751ValGln: 1.751 ± 0.052	3.955ValArg: 3.955 ± 0.092	4.896ValSer: 4.896 ± 0.079	4.646ValThr: 4.646 ± 0.103	5.025ValVal: 5.025 ± 0.095	0.739ValTrp: 0.739 ± 0.033	2.767ValTyr: 2.767 ± 0.076	0.0ValXaa: 0.0 ± 0.0
Trp	0.815TrpAla: 0.815 ± 0.039	0.208TrpCys: 0.208 ± 0.018	0.737TrpAsp: 0.737 ± 0.036	0.581TrpGlu: 0.581 ± 0.034	0.412TrpPhe: 0.412 ± 0.025	0.828TrpGly: 0.828 ± 0.041	0.345TrpHis: 0.345 ± 0.02	0.645TrpIle: 0.645 ± 0.034	0.527TrpLys: 0.527 ± 0.027	1.099TrpLeu: 1.099 ± 0.041	0.342TrpMet: 0.342 ± 0.023	0.603TrpAsn: 0.603 ± 0.031	0.292TrpPro: 0.292 ± 0.019	0.416TrpGln: 0.416 ± 0.024	0.822TrpArg: 0.822 ± 0.036	0.708TrpSer: 0.708 ± 0.035	0.741TrpThr: 0.741 ± 0.034	0.621TrpVal: 0.621 ± 0.028	0.197TrpTrp: 0.197 ± 0.019	0.483TrpTyr: 0.483 ± 0.031	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.552TyrAla: 3.552 ± 0.084	0.59TyrCys: 0.59 ± 0.03	2.911TyrAsp: 2.911 ± 0.077	1.949TyrGlu: 1.949 ± 0.067	1.663TyrPhe: 1.663 ± 0.046	2.989TyrGly: 2.989 ± 0.067	0.812TyrHis: 0.812 ± 0.039	2.827TyrIle: 2.827 ± 0.062	1.844TyrLys: 1.844 ± 0.05	3.18TyrLeu: 3.18 ± 0.068	0.979TyrMet: 0.979 ± 0.042	2.303TyrAsn: 2.303 ± 0.067	1.614TyrPro: 1.614 ± 0.051	1.002TyrGln: 1.002 ± 0.042	2.312TyrArg: 2.312 ± 0.066	2.552TyrSer: 2.552 ± 0.064	2.803TyrThr: 2.803 ± 0.072	2.67TyrVal: 2.67 ± 0.072	0.455TyrTrp: 0.455 ± 0.03	1.881TyrTyr: 1.881 ± 0.069	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.006XaaAla: 0.006 ± 0.003	0.003XaaCys: 0.003 ± 0.002	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.003XaaGly: 0.003 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.004XaaLeu: 0.004 ± 0.003	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.004XaaPro: 0.004 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.003XaaArg: 0.003 ± 0.002	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.003XaaTyr: 0.003 ± 0.002	0.036XaaXaa: 0.036 ± 0.009

Statistics based on 2055 proteins (701839 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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