Olsenella scatoligenes
Average proteome isoelectric point is 5.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2032 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A100YW27|A0A100YW27_9ACTN Aconitate hydratase OS=Olsenella scatoligenes OX=1299998 GN=acnA PE=3 SV=1
MM1 pKa = 7.32 TGALGLAACGSSTTASSSSTTSSSADD27 pKa = 3.09 ASSASTTSFDD37 pKa = 3.72 GNFVLGFDD45 pKa = 4.5 QDD47 pKa = 4.02 FPPYY51 pKa = 10.44 GYY53 pKa = 10.59 VGNDD57 pKa = 3.14 GSYY60 pKa = 9.83 TGFDD64 pKa = 3.13 IDD66 pKa = 5.22 LAQAVCDD73 pKa = 3.83 RR74 pKa = 11.84 EE75 pKa = 4.39 GWTLVPTPISWDD87 pKa = 3.51 AKK89 pKa = 9.86 DD90 pKa = 3.41 TLLNSGQITCIWNGFTIEE108 pKa = 3.95 GRR110 pKa = 11.84 EE111 pKa = 4.03 DD112 pKa = 3.79 GYY114 pKa = 11.68 AFTDD118 pKa = 4.03 PYY120 pKa = 10.24 MEE122 pKa = 4.12 NRR124 pKa = 11.84 QVVVVPASSDD134 pKa = 3.56 VQQLSDD140 pKa = 3.64 LAGKK144 pKa = 9.69 NVITQADD151 pKa = 3.94 SAALDD156 pKa = 3.67 VLSEE160 pKa = 4.36 GGSQEE165 pKa = 3.99 EE166 pKa = 4.33 LGKK169 pKa = 10.85 SFGNLQTIDD178 pKa = 3.62 NYY180 pKa = 9.73 NTAFMMLEE188 pKa = 4.14 SGQVDD193 pKa = 5.29 AIAIDD198 pKa = 3.77 YY199 pKa = 8.6 PVAVFNIGDD208 pKa = 3.64 KK209 pKa = 10.19 TSEE212 pKa = 4.02 FRR214 pKa = 11.84 ILDD217 pKa = 3.54 EE218 pKa = 4.52 NLNSEE223 pKa = 4.22 HH224 pKa = 6.71 FGVGFANTDD233 pKa = 3.75 DD234 pKa = 4.17 GAQLAKK240 pKa = 9.9 TVEE243 pKa = 4.26 ADD245 pKa = 3.73 LQVLDD250 pKa = 4.16 SEE252 pKa = 4.94 GTVKK256 pKa = 10.27 EE257 pKa = 4.12 LCEE260 pKa = 4.2 KK261 pKa = 10.79 YY262 pKa = 10.55 ADD264 pKa = 3.66 QGVSYY269 pKa = 10.5 DD270 pKa = 3.82 LWCLPKK276 pKa = 10.66 AA277 pKa = 4.01
Molecular weight: 29.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.668
IPC_protein 3.694
Toseland 3.465
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.63
Rodwell 3.516
Grimsley 3.376
Solomon 3.681
Lehninger 3.643
Nozaki 3.808
DTASelect 4.062
Thurlkill 3.516
EMBOSS 3.643
Sillero 3.808
Patrickios 1.125
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.75
Protein with the highest isoelectric point:
>tr|A0A100YWD7|A0A100YWD7_9ACTN Two-component system response regulator OS=Olsenella scatoligenes OX=1299998 GN=AUL39_00770 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.62 RR10 pKa = 11.84 KK11 pKa = 9.56 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.36 GGRR28 pKa = 11.84 KK29 pKa = 8.76 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.12 GRR39 pKa = 11.84 KK40 pKa = 8.12 QLTVV44 pKa = 2.97
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2032
0
2032
684394
37
2041
336.8
36.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.136 ± 0.077
1.533 ± 0.026
6.451 ± 0.048
6.439 ± 0.05
3.418 ± 0.031
8.388 ± 0.051
1.989 ± 0.023
4.724 ± 0.047
3.568 ± 0.048
9.105 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.592 ± 0.027
2.882 ± 0.028
4.343 ± 0.034
2.931 ± 0.026
6.165 ± 0.058
6.235 ± 0.057
5.688 ± 0.055
8.421 ± 0.048
1.124 ± 0.019
2.87 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here