Beihai mantis shrimp virus 2
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KJI5|A0A1L3KJI5_9VIRU Uncharacterized protein OS=Beihai mantis shrimp virus 2 OX=1922429 PE=4 SV=1
MM1 pKa = 7.72 DD2 pKa = 5.36 VIHH5 pKa = 6.03 THH7 pKa = 4.59 SAKK10 pKa = 10.12 PVRR13 pKa = 11.84 AVTFNNSQATPVRR26 pKa = 11.84 VLNMPDD32 pKa = 2.72 ITTLQSEE39 pKa = 3.98 IDD41 pKa = 3.64 YY42 pKa = 10.9 LKK44 pKa = 10.75 RR45 pKa = 11.84 QILALQGDD53 pKa = 4.87 FSQISSINEE62 pKa = 3.31 ILAQGLATQKK72 pKa = 11.18 AFLQRR77 pKa = 11.84 LSNLAATARR86 pKa = 11.84 NNSFTNTNQINQASAIPPGDD106 pKa = 3.59 STLGKK111 pKa = 9.35 PSAISLVYY119 pKa = 10.02 FLLHH123 pKa = 6.7 SMGSSDD129 pKa = 3.78 IKK131 pKa = 10.96 LSSTEE136 pKa = 3.59 IKK138 pKa = 10.91 NFLSQDD144 pKa = 3.28 LNLCKK149 pKa = 9.64 FTNEE153 pKa = 3.81 IFSFGPEE160 pKa = 3.8 ITPKK164 pKa = 10.45 AIEE167 pKa = 4.21 FTVPEE172 pKa = 4.12 PTLEE176 pKa = 4.18 EE177 pKa = 4.21 IYY179 pKa = 10.64 LVSIEE184 pKa = 4.62 PGKK187 pKa = 10.47 DD188 pKa = 3.23 DD189 pKa = 4.42 QSVVLYY195 pKa = 9.98 KK196 pKa = 10.21 FNIISNTWEE205 pKa = 4.11 TEE207 pKa = 4.05 VLGTCAIDD215 pKa = 3.62 FSNFSVTNTQGILQCAYY232 pKa = 9.11 GQFVATSTSNVDD244 pKa = 4.06 LYY246 pKa = 11.43 SQNALLNYY254 pKa = 8.11 TFVRR258 pKa = 11.84 ALEE261 pKa = 4.42 SYY263 pKa = 9.58 TLLLEE268 pKa = 4.14 EE269 pKa = 5.56 LIEE272 pKa = 4.42 SALSSVSQTKK282 pKa = 10.34 LNPIVEE288 pKa = 4.17 EE289 pKa = 3.85 ALRR292 pKa = 11.84 IEE294 pKa = 4.0 WLLLQFQEE302 pKa = 5.17 SLLSMQANGIFYY314 pKa = 10.78 VEE316 pKa = 4.27 STSDD320 pKa = 3.61 YY321 pKa = 10.02 VSEE324 pKa = 4.51 INFSDD329 pKa = 3.54 VTKK332 pKa = 10.23 PLSTVSCQSSINYY345 pKa = 9.61 SVTPEE350 pKa = 4.41 GYY352 pKa = 9.3 VGSVSAEE359 pKa = 3.7 PAFEE363 pKa = 4.52 PEE365 pKa = 4.03 QQEE368 pKa = 4.94 GYY370 pKa = 10.92 SDD372 pKa = 3.66 IKK374 pKa = 11.33 NLDD377 pKa = 3.74 DD378 pKa = 4.72 FLLTVTTHH386 pKa = 6.52 SEE388 pKa = 4.44 LEE390 pKa = 3.75 FHH392 pKa = 7.01 EE393 pKa = 4.99 YY394 pKa = 9.44 IVHH397 pKa = 6.79 EE398 pKa = 4.29 DD399 pKa = 3.3 HH400 pKa = 7.87 DD401 pKa = 4.08 VLEE404 pKa = 5.6 DD405 pKa = 3.64 YY406 pKa = 11.3 RR407 pKa = 11.84 KK408 pKa = 8.35 MTVSQEE414 pKa = 3.92 KK415 pKa = 10.13 PNLHH419 pKa = 6.88 HH420 pKa = 6.74 YY421 pKa = 10.84 VDD423 pKa = 3.99 IKK425 pKa = 10.88 ISGSSFPGMEE435 pKa = 4.36 VDD437 pKa = 4.06 VQPCTVDD444 pKa = 3.14 RR445 pKa = 11.84 DD446 pKa = 3.68 GDD448 pKa = 3.57 ISQVSFDD455 pKa = 3.49 TTYY458 pKa = 11.33 NADD461 pKa = 2.99 GSYY464 pKa = 10.36 QAVASVWVGARR475 pKa = 11.84 GLFYY479 pKa = 10.47 KK480 pKa = 10.86 YY481 pKa = 10.25 EE482 pKa = 4.17 VDD484 pKa = 3.97 GKK486 pKa = 8.36 TSQSGRR492 pKa = 11.84 YY493 pKa = 8.79 KK494 pKa = 9.87 EE495 pKa = 4.23 NKK497 pKa = 8.71 VLSEE501 pKa = 4.09 NEE503 pKa = 3.63 QVEE506 pKa = 4.59 IVTTASLEE514 pKa = 4.0 GDD516 pKa = 4.78 KK517 pKa = 10.4 IDD519 pKa = 4.49 CAQKK523 pKa = 8.59 IHH525 pKa = 6.91 GYY527 pKa = 10.07 ALEE530 pKa = 4.34 FNSFNSVVKK539 pKa = 10.54 ISGFKK544 pKa = 10.01 VRR546 pKa = 11.84 YY547 pKa = 6.5 PKK549 pKa = 10.54 PPVFSFVPSFDD560 pKa = 3.59 KK561 pKa = 11.19 QQTEE565 pKa = 3.92 LMAITFKK572 pKa = 10.13 NTYY575 pKa = 9.6 EE576 pKa = 4.35 SIKK579 pKa = 10.05 LTAEE583 pKa = 4.08 YY584 pKa = 9.52 EE585 pKa = 4.19 QLSARR590 pKa = 11.84 LEE592 pKa = 4.13 ALGKK596 pKa = 9.83 ICSPTLLGAFGGLFGTASNFAKK618 pKa = 10.25 SVKK621 pKa = 9.76 AACTLQKK628 pKa = 10.34 LASGFYY634 pKa = 10.43 GLEE637 pKa = 3.69 EE638 pKa = 4.24 LVQGRR643 pKa = 11.84 YY644 pKa = 9.12 IGAILGFAGASTASLTNKK662 pKa = 9.93 LVRR665 pKa = 11.84 KK666 pKa = 10.12 GSFEE670 pKa = 4.31 NKK672 pKa = 9.63 DD673 pKa = 3.48 DD674 pKa = 5.65 LIGHH678 pKa = 5.33 VAKK681 pKa = 10.04 TIRR684 pKa = 11.84 FSKK687 pKa = 10.39 YY688 pKa = 9.89 LPEE691 pKa = 4.27 LNKK694 pKa = 10.4 RR695 pKa = 11.84 KK696 pKa = 9.97 KK697 pKa = 10.28 NYY699 pKa = 9.84 SNSPFEE705 pKa = 4.3 TMSTSDD711 pKa = 3.29 NFIAKK716 pKa = 8.9 MGAPINQLPGSEE728 pKa = 4.86 FITNAFLNVTPEE740 pKa = 3.99 DD741 pKa = 3.59 KK742 pKa = 10.55 SYY744 pKa = 11.09 KK745 pKa = 9.9 RR746 pKa = 11.84 IMKK749 pKa = 9.88 IKK751 pKa = 9.09 EE752 pKa = 3.64 DD753 pKa = 4.27 GYY755 pKa = 10.9 FPEE758 pKa = 4.6 HH759 pKa = 6.53 QSIFTNTMFTVGPDD773 pKa = 2.91 VDD775 pKa = 4.0 TEE777 pKa = 4.28 EE778 pKa = 4.9 LFVMQCRR785 pKa = 11.84 YY786 pKa = 10.11 GISDD790 pKa = 3.53 GAVNRR795 pKa = 11.84 IHH797 pKa = 6.93 SFNTGGSSAKK807 pKa = 9.15 TVAGPGSSFLIKK819 pKa = 10.06 KK820 pKa = 10.22 DD821 pKa = 3.37 GEE823 pKa = 4.33 TSVPYY828 pKa = 10.6 LINGKK833 pKa = 8.39 IEE835 pKa = 4.13 KK836 pKa = 9.12 MALLAMDD843 pKa = 5.76 GYY845 pKa = 11.21 DD846 pKa = 3.03 PSLFTEE852 pKa = 4.53 EE853 pKa = 4.61 MSDD856 pKa = 3.41 TDD858 pKa = 5.84 LEE860 pKa = 4.83 SIDD863 pKa = 4.11 NRR865 pKa = 11.84 TNMEE869 pKa = 3.76 IALSIDD875 pKa = 3.07 KK876 pKa = 10.74 KK877 pKa = 9.89 MKK879 pKa = 9.83 FYY881 pKa = 10.53 NSRR884 pKa = 11.84 VIEE887 pKa = 4.13 SSPFQSTPEE896 pKa = 3.91 ANRR899 pKa = 11.84 DD900 pKa = 3.41 ALASLMSGSLFKK912 pKa = 10.93 DD913 pKa = 3.94 FNYY916 pKa = 10.49 SIPNRR921 pKa = 11.84 TCQPFADD928 pKa = 3.32 ALYY931 pKa = 10.65 NNMLKK936 pKa = 10.13 SEE938 pKa = 4.08 QAPILGGFDD947 pKa = 3.3 FNSRR951 pKa = 11.84 HH952 pKa = 6.24 RR953 pKa = 11.84 LTLEE957 pKa = 3.71 PMSYY961 pKa = 11.5 ADD963 pKa = 6.06 LDD965 pKa = 4.1 DD966 pKa = 6.55 LMLQTYY972 pKa = 10.21 RR973 pKa = 11.84 GILSFDD979 pKa = 2.88 IFVINNII986 pKa = 3.91
Molecular weight: 109.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.785
IPC2_protein 4.825
IPC_protein 4.787
Toseland 4.66
ProMoST 4.889
Dawson 4.736
Bjellqvist 4.876
Wikipedia 4.596
Rodwell 4.647
Grimsley 4.571
Solomon 4.736
Lehninger 4.685
Nozaki 4.838
DTASelect 4.991
Thurlkill 4.647
EMBOSS 4.622
Sillero 4.914
Patrickios 3.795
IPC_peptide 4.749
IPC2_peptide 4.914
IPC2.peptide.svr19 4.854
Protein with the highest isoelectric point:
>tr|A0A1L3KJJ1|A0A1L3KJJ1_9VIRU Uncharacterized protein OS=Beihai mantis shrimp virus 2 OX=1922429 PE=4 SV=1
MM1 pKa = 7.67 SSTNAYY7 pKa = 9.64 VLNLRR12 pKa = 11.84 AKK14 pKa = 9.51 DD15 pKa = 3.48 AKK17 pKa = 11.04 LEE19 pKa = 4.06 IVKK22 pKa = 10.3 DD23 pKa = 3.65 FTMVPGRR30 pKa = 11.84 DD31 pKa = 3.78 PLEE34 pKa = 4.46 HH35 pKa = 6.57 RR36 pKa = 11.84 CKK38 pKa = 10.4 ATICVKK44 pKa = 10.3 GKK46 pKa = 9.81 HH47 pKa = 5.33 FEE49 pKa = 4.38 VVQSAQSKK57 pKa = 8.31 KK58 pKa = 10.36 AAYY61 pKa = 8.18 EE62 pKa = 3.93 KK63 pKa = 10.91 AAGEE67 pKa = 4.4 LLQLWPYY74 pKa = 10.11 VEE76 pKa = 4.69 EE77 pKa = 4.24 VLNEE81 pKa = 3.87 QLNEE85 pKa = 4.09 EE86 pKa = 4.65 EE87 pKa = 4.76 LTVLDD92 pKa = 3.76 INVVKK97 pKa = 9.04 TNKK100 pKa = 8.99 MFSVYY105 pKa = 10.16 RR106 pKa = 11.84 GSTQKK111 pKa = 10.55 NFKK114 pKa = 10.07 KK115 pKa = 10.63 KK116 pKa = 10.36 SDD118 pKa = 3.48 LLTYY122 pKa = 10.59 LEE124 pKa = 5.08 NILL127 pKa = 4.55
Molecular weight: 14.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.348
IPC2_protein 7.629
IPC_protein 7.761
Toseland 7.863
ProMoST 8.112
Dawson 8.434
Bjellqvist 8.478
Wikipedia 8.404
Rodwell 8.448
Grimsley 7.6
Solomon 8.712
Lehninger 8.712
Nozaki 8.682
DTASelect 8.375
Thurlkill 8.463
EMBOSS 8.668
Sillero 8.712
Patrickios 4.609
IPC_peptide 8.712
IPC2_peptide 7.337
IPC2.peptide.svr19 7.522
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3860
127
2185
772.0
86.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.658 ± 0.248
1.58 ± 0.374
5.984 ± 0.287
6.088 ± 0.375
5.855 ± 0.331
4.948 ± 0.451
2.021 ± 0.251
5.881 ± 0.329
6.917 ± 0.59
9.145 ± 0.36
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.684 ± 0.24
5.648 ± 0.511
5.181 ± 0.49
3.497 ± 0.282
3.886 ± 0.586
7.124 ± 1.259
6.969 ± 1.001
6.477 ± 0.465
0.622 ± 0.13
3.834 ± 0.255
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here