Amino acid dipepetide frequency for Ascobolus immersus RN42

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.384AlaAla: 7.384 ± 0.049	1.002AlaCys: 1.002 ± 0.012	3.681AlaAsp: 3.681 ± 0.024	5.237AlaGlu: 5.237 ± 0.035	2.818AlaPhe: 2.818 ± 0.023	4.944AlaGly: 4.944 ± 0.031	1.655AlaHis: 1.655 ± 0.014	3.714AlaIle: 3.714 ± 0.025	4.075AlaLys: 4.075 ± 0.025	6.787AlaLeu: 6.787 ± 0.034	1.626AlaMet: 1.626 ± 0.015	2.665AlaAsn: 2.665 ± 0.022	5.053AlaPro: 5.053 ± 0.039	2.92AlaGln: 2.92 ± 0.023	4.747AlaArg: 4.747 ± 0.028	6.574AlaSer: 6.574 ± 0.035	4.861AlaThr: 4.861 ± 0.026	4.656AlaVal: 4.656 ± 0.028	0.945AlaTrp: 0.945 ± 0.013	1.994AlaTyr: 1.994 ± 0.019	0.0AlaXaa: 0.0 ± 0.0
Cys	0.843CysAla: 0.843 ± 0.011	0.349CysCys: 0.349 ± 0.009	0.674CysAsp: 0.674 ± 0.01	0.766CysGlu: 0.766 ± 0.011	0.625CysPhe: 0.625 ± 0.01	1.027CysGly: 1.027 ± 0.015	0.345CysHis: 0.345 ± 0.006	0.696CysIle: 0.696 ± 0.01	0.692CysLys: 0.692 ± 0.012	1.299CysLeu: 1.299 ± 0.015	0.307CysMet: 0.307 ± 0.006	0.492CysAsn: 0.492 ± 0.009	0.775CysPro: 0.775 ± 0.011	0.473CysGln: 0.473 ± 0.008	0.889CysArg: 0.889 ± 0.012	1.065CysSer: 1.065 ± 0.014	0.821CysThr: 0.821 ± 0.01	0.747CysVal: 0.747 ± 0.01	0.261CysTrp: 0.261 ± 0.006	0.441CysTyr: 0.441 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.131AspAla: 4.131 ± 0.026	0.746AspCys: 0.746 ± 0.011	4.219AspAsp: 4.219 ± 0.039	4.844AspGlu: 4.844 ± 0.033	2.164AspPhe: 2.164 ± 0.02	4.044AspGly: 4.044 ± 0.027	1.2AspHis: 1.2 ± 0.014	2.825AspIle: 2.825 ± 0.02	2.599AspLys: 2.599 ± 0.026	4.758AspLeu: 4.758 ± 0.028	1.234AspMet: 1.234 ± 0.015	1.85AspAsn: 1.85 ± 0.015	3.242AspPro: 3.242 ± 0.023	1.67AspGln: 1.67 ± 0.017	3.013AspArg: 3.013 ± 0.024	4.307AspSer: 4.307 ± 0.032	2.967AspThr: 2.967 ± 0.017	3.316AspVal: 3.316 ± 0.025	0.927AspTrp: 0.927 ± 0.012	1.721AspTyr: 1.721 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	5.687GluAla: 5.687 ± 0.036	0.797GluCys: 0.797 ± 0.013	4.839GluAsp: 4.839 ± 0.037	7.687GluGlu: 7.687 ± 0.058	2.239GluPhe: 2.239 ± 0.017	4.41GluGly: 4.41 ± 0.032	1.423GluHis: 1.423 ± 0.014	3.177GluIle: 3.177 ± 0.022	4.667GluLys: 4.667 ± 0.036	5.763GluLeu: 5.763 ± 0.032	1.726GluMet: 1.726 ± 0.018	2.401GluAsn: 2.401 ± 0.02	3.098GluPro: 3.098 ± 0.027	2.589GluGln: 2.589 ± 0.023	4.723GluArg: 4.723 ± 0.036	4.523GluSer: 4.523 ± 0.034	3.728GluThr: 3.728 ± 0.026	4.268GluVal: 4.268 ± 0.029	1.031GluTrp: 1.031 ± 0.012	1.891GluTyr: 1.891 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	2.665PheAla: 2.665 ± 0.023	0.622PheCys: 0.622 ± 0.011	2.223PheAsp: 2.223 ± 0.019	2.348PheGlu: 2.348 ± 0.017	1.611PhePhe: 1.611 ± 0.019	2.667PheGly: 2.667 ± 0.024	0.932PheHis: 0.932 ± 0.011	1.692PheIle: 1.692 ± 0.017	1.687PheLys: 1.687 ± 0.016	3.441PheLeu: 3.441 ± 0.031	0.724PheMet: 0.724 ± 0.01	1.397PheAsn: 1.397 ± 0.014	2.042PhePro: 2.042 ± 0.013	1.337PheGln: 1.337 ± 0.013	2.24PheArg: 2.24 ± 0.017	2.892PheSer: 2.892 ± 0.02	2.229PheThr: 2.229 ± 0.02	2.228PheVal: 2.228 ± 0.019	0.594PheTrp: 0.594 ± 0.009	1.098PheTyr: 1.098 ± 0.013	0.0PheXaa: 0.0 ± 0.0
Gly	4.239GlyAla: 4.239 ± 0.028	0.967GlyCys: 0.967 ± 0.014	3.231GlyAsp: 3.231 ± 0.023	3.97GlyGlu: 3.97 ± 0.023	2.544GlyPhe: 2.544 ± 0.021	5.608GlyGly: 5.608 ± 0.048	1.49GlyHis: 1.49 ± 0.016	3.168GlyIle: 3.168 ± 0.024	3.879GlyLys: 3.879 ± 0.029	5.408GlyLeu: 5.408 ± 0.031	1.592GlyMet: 1.592 ± 0.017	2.453GlyAsn: 2.453 ± 0.022	3.075GlyPro: 3.075 ± 0.027	2.211GlyGln: 2.211 ± 0.022	4.298GlyArg: 4.298 ± 0.029	5.322GlySer: 5.322 ± 0.038	3.78GlyThr: 3.78 ± 0.033	3.959GlyVal: 3.959 ± 0.029	1.175GlyTrp: 1.175 ± 0.015	2.12GlyTyr: 2.12 ± 0.022	0.0GlyXaa: 0.0 ± 0.0
His	1.6HisAla: 1.6 ± 0.015	0.412HisCys: 0.412 ± 0.008	1.223HisAsp: 1.223 ± 0.015	1.334HisGlu: 1.334 ± 0.014	0.967HisPhe: 0.967 ± 0.013	1.56HisGly: 1.56 ± 0.018	1.07HisHis: 1.07 ± 0.018	1.238HisIle: 1.238 ± 0.014	1.026HisLys: 1.026 ± 0.012	2.383HisLeu: 2.383 ± 0.019	0.475HisMet: 0.475 ± 0.008	0.893HisAsn: 0.893 ± 0.009	1.83HisPro: 1.83 ± 0.018	1.148HisGln: 1.148 ± 0.017	1.604HisArg: 1.604 ± 0.017	1.926HisSer: 1.926 ± 0.018	1.417HisThr: 1.417 ± 0.014	1.229HisVal: 1.229 ± 0.013	0.346HisTrp: 0.346 ± 0.006	0.779HisTyr: 0.779 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	3.649IleAla: 3.649 ± 0.025	0.759IleCys: 0.759 ± 0.01	2.681IleAsp: 2.681 ± 0.018	3.015IleGlu: 3.015 ± 0.022	1.824IlePhe: 1.824 ± 0.017	2.721IleGly: 2.721 ± 0.019	1.214IleHis: 1.214 ± 0.012	2.202IleIle: 2.202 ± 0.021	2.278IleLys: 2.278 ± 0.02	4.279IleLeu: 4.279 ± 0.03	0.879IleMet: 0.879 ± 0.012	1.694IleAsn: 1.694 ± 0.015	3.264IlePro: 3.264 ± 0.024	1.832IleGln: 1.832 ± 0.017	3.069IleArg: 3.069 ± 0.023	3.619IleSer: 3.619 ± 0.022	2.929IleThr: 2.929 ± 0.024	2.776IleVal: 2.776 ± 0.021	0.665IleTrp: 0.665 ± 0.009	1.308IleTyr: 1.308 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	4.653LysAla: 4.653 ± 0.028	0.631LysCys: 0.631 ± 0.009	3.178LysAsp: 3.178 ± 0.026	4.612LysGlu: 4.612 ± 0.033	1.614LysPhe: 1.614 ± 0.014	3.265LysGly: 3.265 ± 0.03	1.253LysHis: 1.253 ± 0.014	2.296LysIle: 2.296 ± 0.019	4.473LysLys: 4.473 ± 0.051	4.446LysLeu: 4.446 ± 0.027	1.137LysMet: 1.137 ± 0.013	1.839LysAsn: 1.839 ± 0.017	3.177LysPro: 3.177 ± 0.026	2.053LysGln: 2.053 ± 0.018	4.163LysArg: 4.163 ± 0.03	3.527LysSer: 3.527 ± 0.022	3.11LysThr: 3.11 ± 0.025	3.21LysVal: 3.21 ± 0.025	0.745LysTrp: 0.745 ± 0.011	1.447LysTyr: 1.447 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	6.925LeuAla: 6.925 ± 0.035	1.2LeuCys: 1.2 ± 0.014	4.934LeuAsp: 4.934 ± 0.028	6.218LeuGlu: 6.218 ± 0.04	3.336LeuPhe: 3.336 ± 0.027	5.144LeuGly: 5.144 ± 0.031	2.348LeuHis: 2.348 ± 0.02	3.558LeuIle: 3.558 ± 0.025	4.5LeuLys: 4.5 ± 0.028	8.473LeuLeu: 8.473 ± 0.048	1.635LeuMet: 1.635 ± 0.017	2.952LeuAsn: 2.952 ± 0.022	5.769LeuPro: 5.769 ± 0.039	3.718LeuGln: 3.718 ± 0.027	5.948LeuArg: 5.948 ± 0.034	6.802LeuSer: 6.802 ± 0.038	4.669LeuThr: 4.669 ± 0.031	4.991LeuVal: 4.991 ± 0.034	1.129LeuTrp: 1.129 ± 0.014	2.291LeuTyr: 2.291 ± 0.016	0.0LeuXaa: 0.0 ± 0.0
Met	1.897MetAla: 1.897 ± 0.017	0.225MetCys: 0.225 ± 0.006	1.332MetAsp: 1.332 ± 0.015	1.593MetGlu: 1.593 ± 0.017	0.7MetPhe: 0.7 ± 0.011	1.395MetGly: 1.395 ± 0.014	0.47MetHis: 0.47 ± 0.008	0.839MetIle: 0.839 ± 0.012	1.16MetLys: 1.16 ± 0.013	1.698MetLeu: 1.698 ± 0.016	0.548MetMet: 0.548 ± 0.011	0.719MetAsn: 0.719 ± 0.01	1.247MetPro: 1.247 ± 0.014	0.815MetGln: 0.815 ± 0.013	1.303MetArg: 1.303 ± 0.014	1.719MetSer: 1.719 ± 0.015	1.087MetThr: 1.087 ± 0.013	1.327MetVal: 1.327 ± 0.014	0.244MetTrp: 0.244 ± 0.006	0.471MetTyr: 0.471 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	2.812AsnAla: 2.812 ± 0.02	0.531AsnCys: 0.531 ± 0.009	1.909AsnAsp: 1.909 ± 0.017	2.123AsnGlu: 2.123 ± 0.02	1.307AsnPhe: 1.307 ± 0.014	2.809AsnGly: 2.809 ± 0.024	0.89AsnHis: 0.89 ± 0.012	1.895AsnIle: 1.895 ± 0.017	1.727AsnLys: 1.727 ± 0.014	3.09AsnLeu: 3.09 ± 0.021	0.763AsnMet: 0.763 ± 0.011	1.558AsnAsn: 1.558 ± 0.019	2.616AsnPro: 2.616 ± 0.021	1.324AsnGln: 1.324 ± 0.016	2.124AsnArg: 2.124 ± 0.019	2.87AsnSer: 2.87 ± 0.021	2.28AsnThr: 2.28 ± 0.02	2.031AsnVal: 2.031 ± 0.016	0.565AsnTrp: 0.565 ± 0.01	1.041AsnTyr: 1.041 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	5.382ProAla: 5.382 ± 0.04	0.585ProCys: 0.585 ± 0.01	3.116ProAsp: 3.116 ± 0.022	4.151ProGlu: 4.151 ± 0.031	2.091ProPhe: 2.091 ± 0.018	3.616ProGly: 3.616 ± 0.033	1.563ProHis: 1.563 ± 0.017	2.82ProIle: 2.82 ± 0.021	3.202ProLys: 3.202 ± 0.024	4.824ProLeu: 4.824 ± 0.028	0.985ProMet: 0.985 ± 0.014	2.343ProAsn: 2.343 ± 0.019	6.593ProPro: 6.593 ± 0.073	2.618ProGln: 2.618 ± 0.033	3.489ProArg: 3.489 ± 0.028	6.252ProSer: 6.252 ± 0.046	5.038ProThr: 5.038 ± 0.04	3.908ProVal: 3.908 ± 0.033	0.608ProTrp: 0.608 ± 0.01	1.587ProTyr: 1.587 ± 0.017	0.0ProXaa: 0.0 ± 0.0
Gln	3.121GlnAla: 3.121 ± 0.024	0.448GlnCys: 0.448 ± 0.007	1.785GlnAsp: 1.785 ± 0.017	2.49GlnGlu: 2.49 ± 0.027	1.301GlnPhe: 1.301 ± 0.013	1.974GlnGly: 1.974 ± 0.017	1.201GlnHis: 1.201 ± 0.016	1.753GlnIle: 1.753 ± 0.018	2.124GlnLys: 2.124 ± 0.022	3.448GlnLeu: 3.448 ± 0.023	0.857GlnMet: 0.857 ± 0.013	1.517GlnAsn: 1.517 ± 0.019	2.688GlnPro: 2.688 ± 0.032	2.89GlnGln: 2.89 ± 0.062	2.551GlnArg: 2.551 ± 0.022	2.902GlnSer: 2.902 ± 0.025	2.387GlnThr: 2.387 ± 0.02	2.021GlnVal: 2.021 ± 0.021	0.485GlnTrp: 0.485 ± 0.008	1.129GlnTyr: 1.129 ± 0.013	0.0GlnXaa: 0.0 ± 0.0
Arg	4.375ArgAla: 4.375 ± 0.026	0.92ArgCys: 0.92 ± 0.013	3.238ArgAsp: 3.238 ± 0.029	4.4ArgGlu: 4.4 ± 0.032	2.343ArgPhe: 2.343 ± 0.021	3.647ArgGly: 3.647 ± 0.028	1.631ArgHis: 1.631 ± 0.016	3.192ArgIle: 3.192 ± 0.022	4.232ArgLys: 4.232 ± 0.032	5.807ArgLeu: 5.807 ± 0.032	1.482ArgMet: 1.482 ± 0.015	2.523ArgAsn: 2.523 ± 0.018	3.733ArgPro: 3.733 ± 0.029	2.674ArgGln: 2.674 ± 0.023	5.569ArgArg: 5.569 ± 0.044	5.014ArgSer: 5.014 ± 0.032	3.608ArgThr: 3.608 ± 0.023	3.376ArgVal: 3.376 ± 0.021	0.946ArgTrp: 0.946 ± 0.013	1.855ArgTyr: 1.855 ± 0.017	0.0ArgXaa: 0.0 ± 0.0
Ser	5.774SerAla: 5.774 ± 0.034	0.965SerCys: 0.965 ± 0.013	4.367SerAsp: 4.367 ± 0.029	4.781SerGlu: 4.781 ± 0.035	3.035SerPhe: 3.035 ± 0.024	5.022SerGly: 5.022 ± 0.034	1.933SerHis: 1.933 ± 0.017	3.812SerIle: 3.812 ± 0.027	4.001SerLys: 4.001 ± 0.028	6.669SerLeu: 6.669 ± 0.034	1.496SerMet: 1.496 ± 0.015	3.012SerAsn: 3.012 ± 0.024	5.638SerPro: 5.638 ± 0.052	3.012SerGln: 3.012 ± 0.028	5.043SerArg: 5.043 ± 0.036	8.863SerSer: 8.863 ± 0.069	6.305SerThr: 6.305 ± 0.045	4.273SerVal: 4.273 ± 0.03	0.944SerTrp: 0.944 ± 0.012	2.045SerTyr: 2.045 ± 0.019	0.0SerXaa: 0.0 ± 0.0
Thr	4.958ThrAla: 4.958 ± 0.035	0.801ThrCys: 0.801 ± 0.012	2.911ThrAsp: 2.911 ± 0.02	3.556ThrGlu: 3.556 ± 0.028	2.28ThrPhe: 2.28 ± 0.018	3.906ThrGly: 3.906 ± 0.032	1.389ThrHis: 1.389 ± 0.015	3.141ThrIle: 3.141 ± 0.023	3.055ThrLys: 3.055 ± 0.022	5.134ThrLeu: 5.134 ± 0.03	1.126ThrMet: 1.126 ± 0.012	2.166ThrAsn: 2.166 ± 0.018	5.245ThrPro: 5.245 ± 0.046	2.045ThrGln: 2.045 ± 0.018	3.401ThrArg: 3.401 ± 0.022	5.703ThrSer: 5.703 ± 0.041	5.255ThrThr: 5.255 ± 0.066	3.797ThrVal: 3.797 ± 0.028	0.71ThrTrp: 0.71 ± 0.008	1.58ThrTyr: 1.58 ± 0.015	0.0ThrXaa: 0.0 ± 0.0
Val	4.45ValAla: 4.45 ± 0.029	0.876ValCys: 0.876 ± 0.012	3.561ValAsp: 3.561 ± 0.023	4.663ValGlu: 4.663 ± 0.03	2.15ValPhe: 2.15 ± 0.019	3.857ValGly: 3.857 ± 0.028	1.361ValHis: 1.361 ± 0.015	2.567ValIle: 2.567 ± 0.022	3.201ValLys: 3.201 ± 0.023	5.142ValLeu: 5.142 ± 0.032	1.237ValMet: 1.237 ± 0.012	1.994ValAsn: 1.994 ± 0.018	3.586ValPro: 3.586 ± 0.026	2.222ValGln: 2.222 ± 0.019	3.593ValArg: 3.593 ± 0.025	4.155ValSer: 4.155 ± 0.027	3.239ValThr: 3.239 ± 0.028	4.207ValVal: 4.207 ± 0.027	0.87ValTrp: 0.87 ± 0.011	1.664ValTyr: 1.664 ± 0.017	0.0ValXaa: 0.0 ± 0.0
Trp	0.901TrpAla: 0.901 ± 0.012	0.229TrpCys: 0.229 ± 0.006	0.924TrpAsp: 0.924 ± 0.012	1.065TrpGlu: 1.065 ± 0.014	0.529TrpPhe: 0.529 ± 0.009	0.873TrpGly: 0.873 ± 0.012	0.316TrpHis: 0.316 ± 0.007	0.724TrpIle: 0.724 ± 0.01	0.957TrpLys: 0.957 ± 0.013	1.164TrpLeu: 1.164 ± 0.013	0.387TrpMet: 0.387 ± 0.007	0.645TrpAsn: 0.645 ± 0.009	0.538TrpPro: 0.538 ± 0.009	0.471TrpGln: 0.471 ± 0.008	0.978TrpArg: 0.978 ± 0.012	0.91TrpSer: 0.91 ± 0.012	0.779TrpThr: 0.779 ± 0.009	0.83TrpVal: 0.83 ± 0.011	0.275TrpTrp: 0.275 ± 0.007	0.414TrpTyr: 0.414 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.891TyrAla: 1.891 ± 0.017	0.525TyrCys: 0.525 ± 0.009	1.752TyrAsp: 1.752 ± 0.018	1.811TyrGlu: 1.811 ± 0.018	1.189TyrPhe: 1.189 ± 0.015	2.001TyrGly: 2.001 ± 0.019	0.774TyrHis: 0.774 ± 0.012	1.352TyrIle: 1.352 ± 0.014	1.297TyrLys: 1.297 ± 0.013	2.527TyrLeu: 2.527 ± 0.022	0.574TyrMet: 0.574 ± 0.01	1.149TyrAsn: 1.149 ± 0.013	1.559TyrPro: 1.559 ± 0.016	1.049TyrGln: 1.049 ± 0.014	1.82TyrArg: 1.82 ± 0.018	2.045TyrSer: 2.045 ± 0.02	1.669TyrThr: 1.669 ± 0.016	1.446TyrVal: 1.446 ± 0.015	0.444TyrTrp: 0.444 ± 0.009	0.978TyrTyr: 0.978 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.018XaaXaa: 0.018 ± 0.008

Statistics based on 17778 proteins (7298591 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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