Pseudoalteromonas phage XC
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G6XV45|A0A6G6XV45_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage XC OX=2712934 PE=4 SV=1
MM1 pKa = 7.87 AFTLGTVKK9 pKa = 10.69 AYY11 pKa = 10.08 GATGNDD17 pKa = 2.95 ILIQAKK23 pKa = 9.53 IDD25 pKa = 3.7 AFADD29 pKa = 4.24 TYY31 pKa = 11.92 ACLTSSYY38 pKa = 9.27 PQAIADD44 pKa = 5.06 DD45 pKa = 3.93 IANSYY50 pKa = 9.62 IAGSLMSASGSTQVTSRR67 pKa = 11.84 KK68 pKa = 9.69 APNGASTNYY77 pKa = 9.58 KK78 pKa = 8.47 QGRR81 pKa = 11.84 YY82 pKa = 9.14 GDD84 pKa = 3.51 SGEE87 pKa = 4.12 YY88 pKa = 10.77 DD89 pKa = 3.02 NGLLANAYY97 pKa = 10.33 LNDD100 pKa = 3.76 YY101 pKa = 9.31 NFCLPIDD108 pKa = 3.53 RR109 pKa = 11.84 SVFFIGVAGTTYY121 pKa = 10.99 DD122 pKa = 4.02 ADD124 pKa = 3.76 NPQQ127 pKa = 2.83
Molecular weight: 13.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.005
IPC2_protein 4.126
IPC_protein 4.075
Toseland 3.834
ProMoST 4.279
Dawson 4.101
Bjellqvist 4.253
Wikipedia 4.101
Rodwell 3.897
Grimsley 3.745
Solomon 4.088
Lehninger 4.037
Nozaki 4.228
DTASelect 4.546
Thurlkill 3.923
EMBOSS 4.101
Sillero 4.202
Patrickios 0.299
IPC_peptide 4.075
IPC2_peptide 4.164
IPC2.peptide.svr19 4.083
Protein with the highest isoelectric point:
>tr|A0A6G6XU83|A0A6G6XU83_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage XC OX=2712934 PE=4 SV=1
MM1 pKa = 7.47 NNMSDD6 pKa = 3.95 LSFTQTSRR14 pKa = 11.84 AMSSSHH20 pKa = 6.79 LSRR23 pKa = 11.84 INHH26 pKa = 6.46 EE27 pKa = 4.14 YY28 pKa = 10.22 RR29 pKa = 11.84 PIWVIAKK36 pKa = 10.09 ANNAKK41 pKa = 9.68 PKK43 pKa = 10.17 QVIDD47 pKa = 3.66 RR48 pKa = 11.84 RR49 pKa = 11.84 AKK51 pKa = 8.72 GWRR54 pKa = 11.84 SNSTVFTGCC63 pKa = 3.86
Molecular weight: 7.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.853
IPC_protein 10.862
Toseland 11.14
ProMoST 11.111
Dawson 11.169
Bjellqvist 10.965
Wikipedia 11.462
Rodwell 11.33
Grimsley 11.213
Solomon 11.433
Lehninger 11.389
Nozaki 11.111
DTASelect 10.965
Thurlkill 11.111
EMBOSS 11.564
Sillero 11.125
Patrickios 11.125
IPC_peptide 11.447
IPC2_peptide 10.189
IPC2.peptide.svr19 8.691
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
73
0
73
14415
40
1320
197.5
22.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.846 ± 0.445
1.346 ± 0.173
6.604 ± 0.226
7.034 ± 0.222
4.037 ± 0.153
6.486 ± 0.177
1.769 ± 0.18
6.153 ± 0.255
7.347 ± 0.444
7.874 ± 0.362
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.65 ± 0.21
5.744 ± 0.207
3.101 ± 0.159
3.531 ± 0.186
3.926 ± 0.192
6.847 ± 0.265
5.897 ± 0.341
6.41 ± 0.239
1.443 ± 0.118
3.954 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here