Xanthomonas virus OP1
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2NPE0|Q2NPE0_9CAUD HslV family protein OS=Xanthomonas virus OP1 OX=329254 PE=4 SV=1
MM1 pKa = 6.71 MTTKK5 pKa = 10.32 FFKK8 pKa = 9.84 VRR10 pKa = 11.84 ARR12 pKa = 11.84 VVYY15 pKa = 7.79 EE16 pKa = 3.45 TFITVEE22 pKa = 3.87 AADD25 pKa = 4.41 GEE27 pKa = 4.78 TALLAANEE35 pKa = 4.64 FFDD38 pKa = 6.05 DD39 pKa = 4.97 MYY41 pKa = 9.32 TTPDD45 pKa = 3.5 NLEE48 pKa = 4.16 LEE50 pKa = 4.76 VVGEE54 pKa = 4.35 EE55 pKa = 4.72 EE56 pKa = 4.02 VTQYY60 pKa = 11.56 DD61 pKa = 4.06 EE62 pKa = 4.93 YY63 pKa = 11.58 LNQINNLGYY72 pKa = 10.86
Molecular weight: 8.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.793
IPC2_protein 3.91
IPC_protein 3.783
Toseland 3.617
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.617
Grimsley 3.528
Solomon 3.719
Lehninger 3.668
Nozaki 3.872
DTASelect 3.973
Thurlkill 3.656
EMBOSS 3.656
Sillero 3.897
Patrickios 1.85
IPC_peptide 3.719
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|Q2NPF7|Q2NPF7_9CAUD DUF5664 domain-containing protein OS=Xanthomonas virus OP1 OX=329254 PE=4 SV=1
MM1 pKa = 6.98 QAYY4 pKa = 7.33 NTAPVVVVSQTKK16 pKa = 9.9 RR17 pKa = 11.84 GSAFEE22 pKa = 4.36 TVTNTLVGFSINWCANMVILPMFGMPINGTTAFHH56 pKa = 6.29 MGIIFTAISIARR68 pKa = 11.84 GYY70 pKa = 8.56 VLRR73 pKa = 11.84 RR74 pKa = 11.84 VFNKK78 pKa = 8.22 ITEE81 pKa = 4.24 RR82 pKa = 11.84 SLSKK86 pKa = 10.98
Molecular weight: 9.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.867
IPC_protein 10.657
Toseland 10.701
ProMoST 10.526
Dawson 10.818
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 11.082
Grimsley 10.877
Solomon 10.921
Lehninger 10.891
Nozaki 10.687
DTASelect 10.526
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.745
Patrickios 10.906
IPC_peptide 10.921
IPC2_peptide 9.604
IPC2.peptide.svr19 8.373
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
59
0
59
13607
35
1571
230.6
25.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.304 ± 0.569
1.058 ± 0.191
5.901 ± 0.148
5.468 ± 0.354
2.962 ± 0.178
8.047 ± 0.26
1.97 ± 0.273
4.895 ± 0.15
5.335 ± 0.474
8.54 ± 0.338
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.513 ± 0.227
3.946 ± 0.189
3.844 ± 0.222
4.755 ± 0.259
5.321 ± 0.212
6.783 ± 0.28
6.306 ± 0.467
7.158 ± 0.31
1.558 ± 0.144
3.337 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here