Torque teno sus virus 1 (isolate Sd-TTV31)
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q8QVM1|ORF3_TTVI1 Uncharacterized ORF3 protein OS=Torque teno sus virus 1 (isolate Sd-TTV31) OX=766190 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.41 EE3 pKa = 3.35 KK4 pKa = 10.56 DD5 pKa = 3.44 YY6 pKa = 11.27 WEE8 pKa = 4.4 EE9 pKa = 3.78 AWLTSCTSIHH19 pKa = 7.02 DD20 pKa = 3.74 HH21 pKa = 6.84 HH22 pKa = 7.45 CDD24 pKa = 3.28 CGSWRR29 pKa = 11.84 DD30 pKa = 4.0 HH31 pKa = 6.31 LWTLCALDD39 pKa = 4.96 DD40 pKa = 4.87 ADD42 pKa = 4.9 LAAAADD48 pKa = 3.67 IIEE51 pKa = 4.5 RR52 pKa = 11.84 EE53 pKa = 4.08 EE54 pKa = 4.81 ADD56 pKa = 3.34 GGEE59 pKa = 4.28 DD60 pKa = 3.6 FGFVDD65 pKa = 5.31 GDD67 pKa = 3.79 PGDD70 pKa = 3.85 AGGG73 pKa = 4.2
Molecular weight: 8.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.897
IPC_protein 3.872
Toseland 3.656
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.821
Rodwell 3.694
Grimsley 3.567
Solomon 3.859
Lehninger 3.808
Nozaki 3.986
DTASelect 4.228
Thurlkill 3.719
EMBOSS 3.821
Sillero 3.999
Patrickios 1.914
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.886
Protein with the highest isoelectric point:
>sp|Q8QVM0|ORF2_TTVI1 Uncharacterized ORF2 protein OS=Torque teno sus virus 1 (isolate Sd-TTV31) OX=766190 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.79 RR2 pKa = 11.84 FRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 FGRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 YY14 pKa = 7.87 YY15 pKa = 9.54 RR16 pKa = 11.84 KK17 pKa = 9.61 RR18 pKa = 11.84 RR19 pKa = 11.84 GGWRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 FRR27 pKa = 11.84 IRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 PWRR35 pKa = 11.84 RR36 pKa = 11.84 WRR38 pKa = 11.84 VRR40 pKa = 11.84 RR41 pKa = 11.84 WRR43 pKa = 11.84 RR44 pKa = 11.84 SVFRR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 GRR52 pKa = 11.84 RR53 pKa = 11.84 ARR55 pKa = 11.84 PYY57 pKa = 10.21 RR58 pKa = 11.84 ISAWNPKK65 pKa = 7.44 VLRR68 pKa = 11.84 NCRR71 pKa = 11.84 ITGWWPVIQCMDD83 pKa = 2.9 GMEE86 pKa = 4.32 WIKK89 pKa = 10.41 YY90 pKa = 9.99 KK91 pKa = 10.93 PMDD94 pKa = 3.88 LRR96 pKa = 11.84 VEE98 pKa = 4.09 ANRR101 pKa = 11.84 IFDD104 pKa = 3.72 KK105 pKa = 10.88 QGSKK109 pKa = 10.07 IEE111 pKa = 4.16 TEE113 pKa = 3.91 QMGYY117 pKa = 10.97 LMQYY121 pKa = 9.69 GGGWSSGVISLEE133 pKa = 3.71 GLFNEE138 pKa = 3.81 NRR140 pKa = 11.84 LWRR143 pKa = 11.84 NIWSKK148 pKa = 11.77 SNDD151 pKa = 2.93 GMDD154 pKa = 3.68 LVRR157 pKa = 11.84 YY158 pKa = 7.0 FGCRR162 pKa = 11.84 IRR164 pKa = 11.84 LYY166 pKa = 8.47 PTEE169 pKa = 4.0 NQGYY173 pKa = 8.2 LFWYY177 pKa = 8.41 DD178 pKa = 3.52 TEE180 pKa = 4.78 FDD182 pKa = 3.61 EE183 pKa = 5.1 QQRR186 pKa = 11.84 RR187 pKa = 11.84 MLDD190 pKa = 3.49 EE191 pKa = 3.97 YY192 pKa = 9.6 TQPSVMLQAKK202 pKa = 9.11 NSRR205 pKa = 11.84 LIVCKK210 pKa = 9.65 QKK212 pKa = 9.55 MPIRR216 pKa = 11.84 RR217 pKa = 11.84 RR218 pKa = 11.84 VKK220 pKa = 10.4 SIFIPPPAQLTTQWKK235 pKa = 8.5 FQQEE239 pKa = 4.39 LCQFPLFNWACICIDD254 pKa = 3.3 MDD256 pKa = 4.61 TPFDD260 pKa = 4.28 YY261 pKa = 11.38 NGAWRR266 pKa = 11.84 NAWWLMRR273 pKa = 11.84 RR274 pKa = 11.84 LQNGNMEE281 pKa = 4.46 YY282 pKa = 9.92 IEE284 pKa = 4.12 RR285 pKa = 11.84 WGRR288 pKa = 11.84 IPMTGDD294 pKa = 3.21 TEE296 pKa = 4.91 LPPADD301 pKa = 4.08 DD302 pKa = 4.66 FKK304 pKa = 11.68 AGGVNKK310 pKa = 9.91 NFKK313 pKa = 8.64 PTGIQRR319 pKa = 11.84 IYY321 pKa = 10.24 PIVAVCLVEE330 pKa = 4.04 GNKK333 pKa = 9.87 RR334 pKa = 11.84 VVKK337 pKa = 9.52 WATVHH342 pKa = 6.44 NGPIDD347 pKa = 3.43 RR348 pKa = 11.84 WRR350 pKa = 11.84 KK351 pKa = 8.68 KK352 pKa = 7.14 QTGTLKK358 pKa = 10.77 LSALRR363 pKa = 11.84 RR364 pKa = 11.84 LVLRR368 pKa = 11.84 VCSEE372 pKa = 3.81 SEE374 pKa = 4.12 TYY376 pKa = 10.68 YY377 pKa = 11.04 KK378 pKa = 8.65 WTASEE383 pKa = 4.09 FTGAFQQDD391 pKa = 3.37 WWPVSGTEE399 pKa = 4.79 YY400 pKa = 10.41 PLCTIKK406 pKa = 10.17 MEE408 pKa = 4.57 PEE410 pKa = 3.93 FEE412 pKa = 4.24 NPTVEE417 pKa = 4.01 VWSWKK422 pKa = 8.95 ATIPTAGTLKK432 pKa = 10.63 DD433 pKa = 3.78 YY434 pKa = 11.06 FGLSSGQQWKK444 pKa = 8.76 DD445 pKa = 2.72 TDD447 pKa = 3.83 FGRR450 pKa = 11.84 LQLPRR455 pKa = 11.84 SSHH458 pKa = 5.76 NVDD461 pKa = 4.43 FGHH464 pKa = 7.6 KK465 pKa = 10.11 ARR467 pKa = 11.84 FGPFCVKK474 pKa = 10.27 KK475 pKa = 10.35 PPVEE479 pKa = 4.39 FRR481 pKa = 11.84 DD482 pKa = 3.9 SAPNPLNIWVKK493 pKa = 8.38 YY494 pKa = 7.74 TFYY497 pKa = 10.76 FQFGGMYY504 pKa = 9.72 QPPTGIQDD512 pKa = 3.69 PCTSNPTYY520 pKa = 9.73 PVRR523 pKa = 11.84 MVGAVTHH530 pKa = 6.51 PKK532 pKa = 10.14 YY533 pKa = 10.53 AGQGGIATQIGDD545 pKa = 3.65 QGITAASLRR554 pKa = 11.84 AISAAPPNTYY564 pKa = 8.49 TQSAFLKK571 pKa = 10.61 APEE574 pKa = 4.24 TEE576 pKa = 4.12 KK577 pKa = 11.11 EE578 pKa = 4.08 EE579 pKa = 4.14 EE580 pKa = 4.34 RR581 pKa = 11.84 EE582 pKa = 4.23 SEE584 pKa = 4.25 TSFTSAEE591 pKa = 4.16 SSSEE595 pKa = 3.88 GDD597 pKa = 3.7 GSSDD601 pKa = 3.32 DD602 pKa = 3.63 QAEE605 pKa = 3.65 RR606 pKa = 11.84 RR607 pKa = 11.84 AARR610 pKa = 11.84 KK611 pKa = 9.09 RR612 pKa = 11.84 VIKK615 pKa = 10.69 LLLKK619 pKa = 10.46 RR620 pKa = 11.84 LADD623 pKa = 3.62 RR624 pKa = 11.84 PVDD627 pKa = 3.43 NKK629 pKa = 10.19 RR630 pKa = 11.84 RR631 pKa = 11.84 RR632 pKa = 11.84 FSEE635 pKa = 3.98
Molecular weight: 74.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.531
IPC_protein 10.043
Toseland 10.321
ProMoST 10.028
Dawson 10.482
Bjellqvist 10.189
Wikipedia 10.687
Rodwell 10.76
Grimsley 10.555
Solomon 10.54
Lehninger 10.496
Nozaki 10.335
DTASelect 10.175
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.409
Patrickios 10.35
IPC_peptide 10.54
IPC2_peptide 9.151
IPC2.peptide.svr19 8.609
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
932
73
635
310.7
36.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.223 ± 1.363
2.253 ± 0.676
6.76 ± 3.022
6.223 ± 0.917
4.077 ± 0.936
6.652 ± 1.233
1.502 ± 1.11
4.399 ± 0.582
5.794 ± 0.729
6.652 ± 1.21
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.253 ± 0.492
2.897 ± 1.215
6.545 ± 1.26
3.97 ± 1.145
10.408 ± 2.173
6.76 ± 1.842
5.579 ± 0.295
4.185 ± 0.858
3.97 ± 0.559
2.897 ± 1.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here