Thermococcus sibiricus (strain DSM 12597 / MM 739)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Thermococcus; Thermococcus sibiricus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2035 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6A0S9|C6A0S9_THESM Uncharacterized protein OS=Thermococcus sibiricus (strain DSM 12597 / MM 739) OX=604354 GN=TSIB_0156 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 10.6VSVDD6 pKa = 3.16KK7 pKa = 9.87DD8 pKa = 3.5TCIGCGVCASICPDD22 pKa = 3.32VFEE25 pKa = 5.24MDD27 pKa = 5.11DD28 pKa = 3.52EE29 pKa = 5.03GKK31 pKa = 10.29AQALVTEE38 pKa = 4.73TDD40 pKa = 3.75LEE42 pKa = 4.49CAKK45 pKa = 10.14EE46 pKa = 3.89AAEE49 pKa = 4.16SCPTGSITVEE59 pKa = 3.81

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5ZZS0|C5ZZS0_THESM HTH-type transcriptional regulator OS=Thermococcus sibiricus (strain DSM 12597 / MM 739) OX=604354 GN=TSIB_1852 PE=3 SV=1
MM1 pKa = 7.22IMKK4 pKa = 10.12RR5 pKa = 11.84RR6 pKa = 11.84PRR8 pKa = 11.84KK9 pKa = 7.56WKK11 pKa = 10.02KK12 pKa = 8.58KK13 pKa = 10.27GRR15 pKa = 11.84MRR17 pKa = 11.84WKK19 pKa = 9.51WIKK22 pKa = 10.46KK23 pKa = 8.96RR24 pKa = 11.84IRR26 pKa = 11.84RR27 pKa = 11.84LKK29 pKa = 9.33RR30 pKa = 11.84QRR32 pKa = 11.84KK33 pKa = 7.67KK34 pKa = 10.61EE35 pKa = 3.79RR36 pKa = 11.84GIII39 pKa = 3.68

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2035

0

2035

554697

37

1870

272.6

30.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.681 ± 0.052

0.588 ± 0.021

4.533 ± 0.041

8.891 ± 0.07

4.516 ± 0.041

7.174 ± 0.047

1.65 ± 0.022

8.504 ± 0.042

8.261 ± 0.069

10.188 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.026

3.652 ± 0.034

4.085 ± 0.033

1.982 ± 0.024

4.866 ± 0.049

4.923 ± 0.041

4.499 ± 0.045

7.474 ± 0.043

1.172 ± 0.023

3.917 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski