Chamois faeces associated circular DNA virus 1
Average proteome isoelectric point is 5.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A168MFW5|A0A168MFW5_9VIRU Replication associated protein OS=Chamois faeces associated circular DNA virus 1 OX=1843766 PE=4 SV=1
MM1 pKa = 7.41 LTQDD5 pKa = 3.76 VPIISNPAGDD15 pKa = 4.42 DD16 pKa = 3.61 GGVLIQHH23 pKa = 6.75 RR24 pKa = 11.84 EE25 pKa = 3.96 YY26 pKa = 11.01 LCDD29 pKa = 3.85 VISTGSAFAIQNQITINPGNPACFPWLSTIAQNFTQYY66 pKa = 11.03 KK67 pKa = 9.15 LEE69 pKa = 3.82 GMMFNYY75 pKa = 10.03 VSTSGALSTTQALGEE90 pKa = 4.3 IIMAVDD96 pKa = 3.68 YY97 pKa = 11.38 NPAGPNFSSKK107 pKa = 8.37 QQMLNQVFAVSKK119 pKa = 10.69 VPSEE123 pKa = 4.42 DD124 pKa = 3.08 AVCPIEE130 pKa = 5.52 CDD132 pKa = 3.39 PKK134 pKa = 10.08 QTGTGDD140 pKa = 3.56 LLYY143 pKa = 10.5 TRR145 pKa = 11.84 GATIPIGQDD154 pKa = 2.86 PRR156 pKa = 11.84 FYY158 pKa = 11.16 DD159 pKa = 3.66 CGTFSLATQGQTAGVTLGEE178 pKa = 3.95 LWITYY183 pKa = 7.22 QVQLYY188 pKa = 9.34 KK189 pKa = 10.42 PQSLAQLPSLAAPNIFNVRR208 pKa = 11.84 SSPANTALVSLMGGSTGFVSTGEE231 pKa = 4.08 MVPTIPASSSSMTFTTFYY249 pKa = 10.51 PAGTSFGLFCTVTGAGMTAPPNITPGTGTILAGGFPNAASAVRR292 pKa = 11.84 VGAVTEE298 pKa = 4.04 ASFAGRR304 pKa = 11.84 FQCTNSVQQWTLVGAFGLTGGNVQIVGWIRR334 pKa = 11.84 RR335 pKa = 11.84 TSNTSSTWSFLTILRR350 pKa = 11.84 VILSLLSTIRR360 pKa = 11.84 TSRR363 pKa = 11.84 SAMMFRR369 pKa = 11.84 VGG371 pKa = 4.18
Molecular weight: 39.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.335
IPC2_protein 5.753
IPC_protein 5.652
Toseland 5.575
ProMoST 5.881
Dawson 5.766
Bjellqvist 5.83
Wikipedia 5.703
Rodwell 5.715
Grimsley 5.537
Solomon 5.779
Lehninger 5.741
Nozaki 5.944
DTASelect 6.122
Thurlkill 5.931
EMBOSS 5.906
Sillero 6.033
Patrickios 3.274
IPC_peptide 5.779
IPC2_peptide 6.02
IPC2.peptide.svr19 6.107
Protein with the highest isoelectric point:
>tr|A0A168MFW5|A0A168MFW5_9VIRU Replication associated protein OS=Chamois faeces associated circular DNA virus 1 OX=1843766 PE=4 SV=1
MM1 pKa = 7.76 EE2 pKa = 5.08 FPDD5 pKa = 4.05 YY6 pKa = 11.32 LKK8 pKa = 10.86 GDD10 pKa = 4.04 PLSPVYY16 pKa = 10.45 HH17 pKa = 6.53 PNIKK21 pKa = 9.7 KK22 pKa = 10.17 CHH24 pKa = 6.33 DD25 pKa = 3.2 VSGWLKK31 pKa = 10.74 YY32 pKa = 10.03 ISKK35 pKa = 10.64 DD36 pKa = 3.36 GEE38 pKa = 4.19 FVDD41 pKa = 3.93 MTGKK45 pKa = 10.49 FNILDD50 pKa = 3.76 YY51 pKa = 11.2 EE52 pKa = 4.18 VGKK55 pKa = 10.01 RR56 pKa = 11.84 QKK58 pKa = 10.7 AYY60 pKa = 9.86 QDD62 pKa = 3.36 HH63 pKa = 7.22 KK64 pKa = 10.75 FTEE67 pKa = 3.93 DD68 pKa = 2.88 WIRR71 pKa = 11.84 RR72 pKa = 11.84 KK73 pKa = 9.98 NYY75 pKa = 9.7 KK76 pKa = 10.01 EE77 pKa = 3.85 VVFPVLLKK85 pKa = 8.09 TTTGRR90 pKa = 11.84 EE91 pKa = 3.94 YY92 pKa = 11.34 RR93 pKa = 11.84 MNAPDD98 pKa = 3.76 ASVKK102 pKa = 9.16 QRR104 pKa = 11.84 HH105 pKa = 4.9 WWIVAPPNSGKK116 pKa = 7.93 TRR118 pKa = 11.84 WLQDD122 pKa = 3.0 TFDD125 pKa = 3.75 GQRR128 pKa = 11.84 VYY130 pKa = 11.11 VPSKK134 pKa = 10.6 GEE136 pKa = 3.98 YY137 pKa = 9.42 PFEE140 pKa = 6.05 RR141 pKa = 11.84 YY142 pKa = 9.52 DD143 pKa = 3.39 GQSIVVYY150 pKa = 10.01 DD151 pKa = 4.47 DD152 pKa = 3.78 RR153 pKa = 11.84 DD154 pKa = 3.9 SITFEE159 pKa = 3.85 EE160 pKa = 4.93 FSNVTGVYY168 pKa = 10.14 KK169 pKa = 10.46 IEE171 pKa = 3.79 THH173 pKa = 6.1 VYY175 pKa = 8.97 GKK177 pKa = 10.73 ARR179 pKa = 11.84 FVNVFWPEE187 pKa = 3.54 HH188 pKa = 4.33 QARR191 pKa = 11.84 NVIVLSNKK199 pKa = 9.58 TIEE202 pKa = 4.2 QQCGDD207 pKa = 3.49 DD208 pKa = 3.72 SYY210 pKa = 11.98 RR211 pKa = 11.84 MKK213 pKa = 10.76 KK214 pKa = 10.18 RR215 pKa = 11.84 FIQISGAALCPPHH228 pKa = 7.39 DD229 pKa = 4.49 SSDD232 pKa = 4.35 DD233 pKa = 3.64 EE234 pKa = 6.14 DD235 pKa = 4.42 EE236 pKa = 5.14 VADD239 pKa = 4.83 CPPDD243 pKa = 3.36 EE244 pKa = 5.48 HH245 pKa = 8.05 GFASS249 pKa = 3.63
Molecular weight: 29.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.662
IPC2_protein 5.639
IPC_protein 5.664
Toseland 6.059
ProMoST 5.995
Dawson 5.906
Bjellqvist 5.919
Wikipedia 5.919
Rodwell 5.881
Grimsley 6.211
Solomon 5.906
Lehninger 5.906
Nozaki 6.16
DTASelect 6.364
Thurlkill 6.351
EMBOSS 6.313
Sillero 6.262
Patrickios 4.075
IPC_peptide 5.931
IPC2_peptide 6.249
IPC2.peptide.svr19 6.272
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
620
249
371
310.0
34.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.774 ± 1.669
1.774 ± 0.102
5.323 ± 2.369
3.871 ± 1.546
5.323 ± 0.062
8.387 ± 1.43
1.452 ± 1.066
5.645 ± 0.5
4.032 ± 2.421
5.968 ± 1.424
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.419 ± 0.492
4.194 ± 0.35
6.129 ± 0.064
5.161 ± 0.693
4.194 ± 0.865
8.065 ± 1.235
8.387 ± 2.402
7.419 ± 0.614
1.774 ± 0.385
3.71 ± 1.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here