Nesidiocoris tenuis virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L6YQ22|A0A1L6YQ22_9VIRU ORF2 OS=Nesidiocoris tenuis virus OX=1930921 PE=4 SV=1
MM1 pKa = 7.75ILPEE5 pKa = 4.66NPSSLYY11 pKa = 10.09EE12 pKa = 3.82WALNHH17 pKa = 5.86YY18 pKa = 10.68SYY20 pKa = 10.22MIKK23 pKa = 10.16SRR25 pKa = 11.84PKK27 pKa = 9.72PPLTEE32 pKa = 3.92EE33 pKa = 5.39AIDD36 pKa = 4.5EE37 pKa = 4.3YY38 pKa = 11.22TALQTIAYY46 pKa = 8.62HH47 pKa = 6.07EE48 pKa = 4.29KK49 pKa = 9.74FINAIFCPLMRR60 pKa = 11.84EE61 pKa = 4.2VKK63 pKa = 10.12KK64 pKa = 10.81RR65 pKa = 11.84LLAILDD71 pKa = 3.55PRR73 pKa = 11.84FVLFTDD79 pKa = 3.9MSPDD83 pKa = 3.56DD84 pKa = 3.65FADD87 pKa = 3.69RR88 pKa = 11.84LTRR91 pKa = 11.84TFSEE95 pKa = 4.26EE96 pKa = 4.36LFSKK100 pKa = 10.64DD101 pKa = 3.19YY102 pKa = 10.55VGHH105 pKa = 5.83VKK107 pKa = 10.56EE108 pKa = 3.85SDD110 pKa = 2.71IRR112 pKa = 11.84KK113 pKa = 9.16FDD115 pKa = 3.28KK116 pKa = 10.88SQQEE120 pKa = 3.93KK121 pKa = 10.39VLRR124 pKa = 11.84AEE126 pKa = 4.47QKK128 pKa = 10.31VLMLFGFPPQLAEE141 pKa = 3.54LWLRR145 pKa = 11.84VHH147 pKa = 7.19EE148 pKa = 4.15DD149 pKa = 3.18TILIDD154 pKa = 3.74RR155 pKa = 11.84GLGIMFRR162 pKa = 11.84VRR164 pKa = 11.84WHH166 pKa = 6.67RR167 pKa = 11.84KK168 pKa = 9.38SGDD171 pKa = 2.98ASTFLGNSLVLLMVLCSTYY190 pKa = 11.37DD191 pKa = 3.26LTKK194 pKa = 10.58AVMVVFPGDD203 pKa = 3.38DD204 pKa = 3.79FYY206 pKa = 11.04IIGPYY211 pKa = 10.09SLAIDD216 pKa = 3.92RR217 pKa = 11.84SAYY220 pKa = 9.85LAYY223 pKa = 10.47NYY225 pKa = 10.46NFEE228 pKa = 5.59SEE230 pKa = 4.28DD231 pKa = 3.52LDD233 pKa = 3.54YY234 pKa = 11.1KK235 pKa = 11.04YY236 pKa = 10.73IYY238 pKa = 9.77FCPKK242 pKa = 9.93FLPISIGGSVLGFLPTQLSMLSHH265 pKa = 7.48DD266 pKa = 3.74SLPRR270 pKa = 11.84SRR272 pKa = 11.84CSVNGQLDD280 pKa = 3.65QSAHH284 pKa = 5.73GARR287 pKa = 11.84CHH289 pKa = 4.88EE290 pKa = 4.29RR291 pKa = 11.84EE292 pKa = 3.96RR293 pKa = 11.84RR294 pKa = 3.57

Molecular weight: 34.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L6YQ22|A0A1L6YQ22_9VIRU ORF2 OS=Nesidiocoris tenuis virus OX=1930921 PE=4 SV=1
MM1 pKa = 7.6EE2 pKa = 5.45NLSVMYY8 pKa = 9.85QAVRR12 pKa = 11.84KK13 pKa = 10.0AMTIRR18 pKa = 11.84TPNSGKK24 pKa = 10.3LLNTEE29 pKa = 3.91TTRR32 pKa = 11.84LLRR35 pKa = 11.84SSNKK39 pKa = 10.23DD40 pKa = 3.07YY41 pKa = 11.54GLYY44 pKa = 10.14LVKK47 pKa = 10.15TGVFLLSPLARR58 pKa = 11.84DD59 pKa = 3.3HH60 pKa = 6.87RR61 pKa = 11.84FAYY64 pKa = 10.35DD65 pKa = 3.6GEE67 pKa = 4.24KK68 pKa = 10.42LVCFEE73 pKa = 4.94NKK75 pKa = 9.04MKK77 pKa = 9.86LTTTTNSDD85 pKa = 3.79YY86 pKa = 11.36LIVNCDD92 pKa = 3.49TEE94 pKa = 5.29RR95 pKa = 11.84MLEE98 pKa = 4.02RR99 pKa = 11.84QFYY102 pKa = 10.41SALKK106 pKa = 10.57NITLPTRR113 pKa = 11.84LPKK116 pKa = 10.4LRR118 pKa = 11.84FVLGVPGCGKK128 pKa = 8.65STYY131 pKa = 8.95IAKK134 pKa = 10.33ARR136 pKa = 11.84LPHH139 pKa = 5.93EE140 pKa = 4.65KK141 pKa = 10.1VWTATRR147 pKa = 11.84LGKK150 pKa = 10.18SDD152 pKa = 3.85VIKK155 pKa = 10.51KK156 pKa = 10.26IKK158 pKa = 9.8GDD160 pKa = 3.21KK161 pKa = 11.14DD162 pKa = 3.35MVRR165 pKa = 11.84TIGSILIKK173 pKa = 10.5GDD175 pKa = 3.05NTMTHH180 pKa = 6.36MKK182 pKa = 9.74NQPTRR187 pKa = 11.84VFVDD191 pKa = 3.92EE192 pKa = 5.22VIMAHH197 pKa = 6.42AGEE200 pKa = 4.5VMARR204 pKa = 11.84AQEE207 pKa = 3.95LDD209 pKa = 3.49IDD211 pKa = 4.13EE212 pKa = 5.05MICLGDD218 pKa = 3.99LKK220 pKa = 10.64QIPFIARR227 pKa = 11.84IAEE230 pKa = 4.29VKK232 pKa = 10.17LHH234 pKa = 5.47YY235 pKa = 10.85SKK237 pKa = 10.81ISDD240 pKa = 3.65MANKK244 pKa = 10.21DD245 pKa = 3.26IEE247 pKa = 4.42FLTNSHH253 pKa = 6.69RR254 pKa = 11.84VPADD258 pKa = 2.78IAVVLTRR265 pKa = 11.84YY266 pKa = 9.24FYY268 pKa = 11.31KK269 pKa = 10.86DD270 pKa = 3.19FVGNEE275 pKa = 4.1KK276 pKa = 10.26IKK278 pKa = 10.98LQTDD282 pKa = 3.37VV283 pKa = 3.79

Molecular weight: 32.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

577

283

294

288.5

33.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.719 ± 0.185

1.56 ± 0.094

6.586 ± 0.145

5.546 ± 0.386

4.853 ± 0.85

4.679 ± 0.172

2.426 ± 0.197

6.239 ± 0.306

7.106 ± 1.341

11.265 ± 0.655

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.64 ± 0.387

3.466 ± 0.726

4.333 ± 0.742

2.426 ± 0.197

6.586 ± 0.145

6.412 ± 1.171

5.893 ± 1.439

6.239 ± 0.761

0.693 ± 0.219

4.333 ± 0.515
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski