Propionicimonas paludicola
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2964 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A9CST5|A0A2A9CST5_9ACTN Pyruvate carboxylase OS=Propionicimonas paludicola OX=185243 GN=ATK74_2008 PE=4 SV=1
MM1 pKa = 7.54 AGVDD5 pKa = 3.49 PHH7 pKa = 8.42 FEE9 pKa = 4.04 EE10 pKa = 5.6 LDD12 pKa = 3.3 AWVSAGVRR20 pKa = 11.84 QVVPIAEE27 pKa = 4.36 LAGLFTPVYY36 pKa = 9.06 DD37 pKa = 3.82 QVSAAITQAGAVMVGPAYY55 pKa = 10.34 AEE57 pKa = 4.1 YY58 pKa = 10.12 FAMPEE63 pKa = 3.91 DD64 pKa = 4.09 TVDD67 pKa = 3.33 VEE69 pKa = 4.24 IGFGIAEE76 pKa = 4.07 PTAIDD81 pKa = 3.84 GLNVRR86 pKa = 11.84 QRR88 pKa = 11.84 PATTAVVGTHH98 pKa = 5.97 FGGYY102 pKa = 10.24 DD103 pKa = 3.48 SLDD106 pKa = 3.23 EE107 pKa = 4.69 SYY109 pKa = 11.67 AEE111 pKa = 3.83 LMPWLEE117 pKa = 4.23 SQDD120 pKa = 3.76 VALGDD125 pKa = 5.44 SMFEE129 pKa = 4.15 WYY131 pKa = 10.46 LSEE134 pKa = 4.79 PDD136 pKa = 3.59 EE137 pKa = 4.78 APEE140 pKa = 3.9 NTVTKK145 pKa = 10.56 LVFPLAA151 pKa = 4.15
Molecular weight: 16.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.749
IPC2_protein 3.694
IPC_protein 3.643
Toseland 3.452
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.541
Rodwell 3.478
Grimsley 3.363
Solomon 3.605
Lehninger 3.567
Nozaki 3.745
DTASelect 3.91
Thurlkill 3.503
EMBOSS 3.554
Sillero 3.77
Patrickios 0.846
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.7
Protein with the highest isoelectric point:
>tr|A0A2A9CUZ0|A0A2A9CUZ0_9ACTN Uncharacterized protein OS=Propionicimonas paludicola OX=185243 GN=ATK74_2530 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 THH17 pKa = 6.18 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIISARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 QGRR40 pKa = 11.84 ARR42 pKa = 11.84 LAGG45 pKa = 3.61
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.524
IPC2_protein 11.374
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.676
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.398
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.293
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2964
0
2964
985853
31
5968
332.6
35.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.443 ± 0.058
0.72 ± 0.013
5.584 ± 0.035
5.269 ± 0.049
2.973 ± 0.022
8.805 ± 0.035
1.855 ± 0.021
4.235 ± 0.035
2.373 ± 0.033
10.833 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.786 ± 0.021
2.148 ± 0.026
5.586 ± 0.034
3.227 ± 0.021
6.769 ± 0.051
5.963 ± 0.046
6.003 ± 0.068
8.686 ± 0.041
1.631 ± 0.022
2.109 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here