Bacillus phage vB_BthP-Goe4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Northropvirinae; Claudivirus; Bacillus virus Goe4

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KTA4|A0A386KTA4_9CAUD Uncharacterized protein OS=Bacillus phage vB_BthP-Goe4 OX=2315470 GN=Goe4_c00180 PE=4 SV=1
MM1 pKa = 7.74AIVTKK6 pKa = 10.04TLTFEE11 pKa = 4.53GKK13 pKa = 9.95KK14 pKa = 10.16SGSVVEE20 pKa = 4.75NPNIAYY26 pKa = 10.33FNDD29 pKa = 3.36VEE31 pKa = 4.63FTQTQYY37 pKa = 11.64DD38 pKa = 3.58QIYY41 pKa = 9.24GSEE44 pKa = 4.09LRR46 pKa = 11.84VSKK49 pKa = 11.02DD50 pKa = 3.67DD51 pKa = 3.32ILKK54 pKa = 9.0FTFVLPPDD62 pKa = 4.4TILAYY67 pKa = 10.28QEE69 pKa = 3.4GDD71 pKa = 2.65IYY73 pKa = 11.34YY74 pKa = 10.38KK75 pKa = 10.78SKK77 pKa = 11.09LDD79 pKa = 3.97DD80 pKa = 4.27GEE82 pKa = 4.69NEE84 pKa = 4.32LRR86 pKa = 11.84SSDD89 pKa = 3.18KK90 pKa = 11.02GVYY93 pKa = 10.55NNDD96 pKa = 2.72TTTYY100 pKa = 9.18KK101 pKa = 10.53QKK103 pKa = 11.1LLATYY108 pKa = 10.46ASDD111 pKa = 3.98IVDD114 pKa = 3.48EE115 pKa = 4.42NKK117 pKa = 9.88IEE119 pKa = 4.04FTIKK123 pKa = 9.5STGDD127 pKa = 3.09YY128 pKa = 10.8GIYY131 pKa = 9.35EE132 pKa = 4.18VKK134 pKa = 10.15IDD136 pKa = 5.1VIYY139 pKa = 10.9NDD141 pKa = 4.2NGLNSYY147 pKa = 9.56GGQYY151 pKa = 10.21QYY153 pKa = 11.07QEE155 pKa = 3.96SGGTIVTVPIQNKK168 pKa = 9.03KK169 pKa = 10.36LEE171 pKa = 4.61EE172 pKa = 4.1IVTEE176 pKa = 4.32LEE178 pKa = 4.09GRR180 pKa = 11.84PSNSITNVTFAQIGVEE196 pKa = 4.09IVDD199 pKa = 3.52

Molecular weight: 22.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KQ40|A0A386KQ40_9CAUD Uncharacterized protein OS=Bacillus phage vB_BthP-Goe4 OX=2315470 GN=Goe4_c00190 PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 10.3RR3 pKa = 11.84QVRR6 pKa = 11.84VMLKK10 pKa = 9.99EE11 pKa = 3.89LNRR14 pKa = 11.84LNKK17 pKa = 9.73IYY19 pKa = 10.76SKK21 pKa = 10.68NARR24 pKa = 11.84FLHH27 pKa = 5.63SNKK30 pKa = 9.06PRR32 pKa = 11.84AAFNLFVKK40 pKa = 9.72RR41 pKa = 11.84TNLSIEE47 pKa = 4.17LKK49 pKa = 9.38KK50 pKa = 9.96WGYY53 pKa = 10.54FKK55 pKa = 11.21

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

7344

27

726

170.8

19.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.289 ± 0.419

1.225 ± 0.199

6.563 ± 0.337

8.197 ± 0.631

4.493 ± 0.302

6.413 ± 0.484

1.321 ± 0.163

6.958 ± 0.329

9.736 ± 0.7

7.176 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.173 ± 0.249

7.081 ± 0.491

2.465 ± 0.231

3.145 ± 0.216

4.071 ± 0.328

5.188 ± 0.263

6.019 ± 0.438

6.264 ± 0.496

1.253 ± 0.168

4.97 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski