Amino acid dipepetide frequency for Flavobacterium phage vB_FspS_tooticki6-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.597AlaAla: 0.597 ± 0.332
0.683AlaCys: 0.683 ± 0.28
2.389AlaAsp: 2.389 ± 0.451
3.157AlaGlu: 3.157 ± 0.655
2.048AlaPhe: 2.048 ± 0.449
2.218AlaGly: 2.218 ± 0.403
0.597AlaHis: 0.597 ± 0.189
3.498AlaIle: 3.498 ± 0.466
5.46AlaLys: 5.46 ± 0.616
5.29AlaLeu: 5.29 ± 0.669
1.365AlaMet: 1.365 ± 0.447
5.204AlaAsn: 5.204 ± 0.643
0.853AlaPro: 0.853 ± 0.266
2.048AlaGln: 2.048 ± 0.374
1.109AlaArg: 1.109 ± 0.405
3.242AlaSer: 3.242 ± 0.714
4.266AlaThr: 4.266 ± 0.562
3.071AlaVal: 3.071 ± 0.583
0.341AlaTrp: 0.341 ± 0.136
1.621AlaTyr: 1.621 ± 0.35
0.0AlaXaa: 0.0 ± 0.0
Cys
0.085CysAla: 0.085 ± 0.081
0.341CysCys: 0.341 ± 0.177
1.194CysAsp: 1.194 ± 0.335
1.109CysGlu: 1.109 ± 0.259
1.024CysPhe: 1.024 ± 0.309
1.109CysGly: 1.109 ± 0.312
0.171CysHis: 0.171 ± 0.133
0.768CysIle: 0.768 ± 0.289
0.853CysLys: 0.853 ± 0.256
1.28CysLeu: 1.28 ± 0.291
0.085CysMet: 0.085 ± 0.081
0.256CysAsn: 0.256 ± 0.151
0.512CysPro: 0.512 ± 0.19
0.427CysGln: 0.427 ± 0.167
0.256CysArg: 0.256 ± 0.151
0.768CysSer: 0.768 ± 0.246
0.597CysThr: 0.597 ± 0.181
0.427CysVal: 0.427 ± 0.211
0.085CysTrp: 0.085 ± 0.101
0.427CysTyr: 0.427 ± 0.172
0.0CysXaa: 0.0 ± 0.0
Asp
3.925AspAla: 3.925 ± 0.383
0.938AspCys: 0.938 ± 0.235
2.218AspAsp: 2.218 ± 0.352
4.522AspGlu: 4.522 ± 0.584
3.498AspPhe: 3.498 ± 0.505
2.73AspGly: 2.73 ± 0.515
0.768AspHis: 0.768 ± 0.227
4.266AspIle: 4.266 ± 0.657
5.972AspLys: 5.972 ± 0.827
5.204AspLeu: 5.204 ± 0.566
1.28AspMet: 1.28 ± 0.372
4.522AspAsn: 4.522 ± 0.689
0.171AspPro: 0.171 ± 0.123
0.341AspGln: 0.341 ± 0.204
1.621AspArg: 1.621 ± 0.293
3.754AspSer: 3.754 ± 0.532
4.181AspThr: 4.181 ± 0.608
2.986AspVal: 2.986 ± 0.513
0.853AspTrp: 0.853 ± 0.226
2.73AspTyr: 2.73 ± 0.477
0.0AspXaa: 0.0 ± 0.0
Glu
2.815GluAla: 2.815 ± 0.772
0.683GluCys: 0.683 ± 0.295
3.157GluAsp: 3.157 ± 0.612
4.181GluGlu: 4.181 ± 0.765
4.607GluPhe: 4.607 ± 0.763
2.218GluGly: 2.218 ± 0.383
1.109GluHis: 1.109 ± 0.32
6.74GluIle: 6.74 ± 0.733
6.655GluLys: 6.655 ± 0.887
8.19GluLeu: 8.19 ± 0.899
1.792GluMet: 1.792 ± 0.357
6.911GluAsn: 6.911 ± 0.819
1.706GluPro: 1.706 ± 0.348
3.498GluGln: 3.498 ± 0.507
2.815GluArg: 2.815 ± 0.652
3.498GluSer: 3.498 ± 0.534
3.669GluThr: 3.669 ± 0.513
3.754GluVal: 3.754 ± 0.539
0.597GluTrp: 0.597 ± 0.21
3.071GluTyr: 3.071 ± 0.64
0.0GluXaa: 0.0 ± 0.0
Phe
2.73PheAla: 2.73 ± 0.489
0.427PheCys: 0.427 ± 0.168
4.181PheAsp: 4.181 ± 0.622
3.413PheGlu: 3.413 ± 0.678
2.645PhePhe: 2.645 ± 0.377
3.242PheGly: 3.242 ± 0.472
0.512PheHis: 0.512 ± 0.222
3.754PheIle: 3.754 ± 0.632
4.522PheLys: 4.522 ± 0.684
3.925PheLeu: 3.925 ± 0.606
1.024PheMet: 1.024 ± 0.24
4.948PheAsn: 4.948 ± 0.842
1.109PhePro: 1.109 ± 0.314
1.792PheGln: 1.792 ± 0.475
0.938PheArg: 0.938 ± 0.338
4.095PheSer: 4.095 ± 0.563
4.607PheThr: 4.607 ± 0.64
2.389PheVal: 2.389 ± 0.447
0.341PheTrp: 0.341 ± 0.158
1.962PheTyr: 1.962 ± 0.485
0.0PheXaa: 0.0 ± 0.0
Gly
3.157GlyAla: 3.157 ± 0.75
0.512GlyCys: 0.512 ± 0.205
2.901GlyAsp: 2.901 ± 0.517
2.304GlyGlu: 2.304 ± 0.426
3.071GlyPhe: 3.071 ± 0.643
2.304GlyGly: 2.304 ± 0.544
0.512GlyHis: 0.512 ± 0.196
3.327GlyIle: 3.327 ± 0.394
2.986GlyLys: 2.986 ± 0.585
3.413GlyLeu: 3.413 ± 0.485
1.109GlyMet: 1.109 ± 0.309
4.607GlyAsn: 4.607 ± 0.592
0.0GlyPro: 0.0 ± 0.0
1.621GlyGln: 1.621 ± 0.387
1.621GlyArg: 1.621 ± 0.362
3.413GlySer: 3.413 ± 0.602
4.778GlyThr: 4.778 ± 0.797
3.157GlyVal: 3.157 ± 0.428
0.171GlyTrp: 0.171 ± 0.11
2.218GlyTyr: 2.218 ± 0.45
0.0GlyXaa: 0.0 ± 0.0
His
0.341HisAla: 0.341 ± 0.182
0.256HisCys: 0.256 ± 0.15
0.853HisAsp: 0.853 ± 0.282
0.512HisGlu: 0.512 ± 0.176
0.938HisPhe: 0.938 ± 0.271
0.597HisGly: 0.597 ± 0.245
0.341HisHis: 0.341 ± 0.213
0.938HisIle: 0.938 ± 0.258
0.853HisLys: 0.853 ± 0.285
1.024HisLeu: 1.024 ± 0.316
0.171HisMet: 0.171 ± 0.129
1.536HisAsn: 1.536 ± 0.362
0.427HisPro: 0.427 ± 0.231
0.512HisGln: 0.512 ± 0.192
0.683HisArg: 0.683 ± 0.24
0.938HisSer: 0.938 ± 0.314
0.853HisThr: 0.853 ± 0.204
0.512HisVal: 0.512 ± 0.224
0.256HisTrp: 0.256 ± 0.179
0.683HisTyr: 0.683 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
4.181IleAla: 4.181 ± 0.746
1.024IleCys: 1.024 ± 0.319
5.716IleAsp: 5.716 ± 0.577
7.508IleGlu: 7.508 ± 0.959
3.071IlePhe: 3.071 ± 0.543
3.754IleGly: 3.754 ± 0.65
0.938IleHis: 0.938 ± 0.386
5.887IleIle: 5.887 ± 0.881
8.361IleLys: 8.361 ± 0.822
6.313IleLeu: 6.313 ± 0.749
1.365IleMet: 1.365 ± 0.377
6.399IleAsn: 6.399 ± 0.798
2.304IlePro: 2.304 ± 0.425
2.73IleGln: 2.73 ± 0.539
1.706IleArg: 1.706 ± 0.376
5.375IleSer: 5.375 ± 0.694
4.778IleThr: 4.778 ± 0.73
4.522IleVal: 4.522 ± 0.675
0.853IleTrp: 0.853 ± 0.307
3.669IleTyr: 3.669 ± 0.516
0.0IleXaa: 0.0 ± 0.0
Lys
4.692LysAla: 4.692 ± 0.576
1.28LysCys: 1.28 ± 0.373
5.29LysAsp: 5.29 ± 0.757
8.361LysGlu: 8.361 ± 1.213
3.669LysPhe: 3.669 ± 0.447
4.692LysGly: 4.692 ± 0.596
1.621LysHis: 1.621 ± 0.364
7.593LysIle: 7.593 ± 0.772
7.934LysLys: 7.934 ± 0.821
7.252LysLeu: 7.252 ± 0.781
3.498LysMet: 3.498 ± 0.494
5.631LysAsn: 5.631 ± 0.599
2.73LysPro: 2.73 ± 0.516
4.692LysGln: 4.692 ± 0.83
2.815LysArg: 2.815 ± 0.507
5.46LysSer: 5.46 ± 0.654
6.569LysThr: 6.569 ± 0.831
5.46LysVal: 5.46 ± 0.74
1.194LysTrp: 1.194 ± 0.329
4.181LysTyr: 4.181 ± 0.528
0.0LysXaa: 0.0 ± 0.0
Leu
3.925LeuAla: 3.925 ± 0.788
0.512LeuCys: 0.512 ± 0.253
5.375LeuAsp: 5.375 ± 0.592
6.399LeuGlu: 6.399 ± 0.887
4.095LeuPhe: 4.095 ± 0.496
3.157LeuGly: 3.157 ± 0.462
0.853LeuHis: 0.853 ± 0.272
7.593LeuIle: 7.593 ± 0.828
9.385LeuLys: 9.385 ± 0.814
7.167LeuLeu: 7.167 ± 0.776
1.621LeuMet: 1.621 ± 0.357
8.19LeuAsn: 8.19 ± 0.759
4.01LeuPro: 4.01 ± 0.561
3.839LeuGln: 3.839 ± 0.516
2.645LeuArg: 2.645 ± 0.485
5.716LeuSer: 5.716 ± 0.761
5.972LeuThr: 5.972 ± 0.799
4.778LeuVal: 4.778 ± 0.717
0.597LeuTrp: 0.597 ± 0.279
3.839LeuTyr: 3.839 ± 0.565
0.0LeuXaa: 0.0 ± 0.0
Met
1.621MetAla: 1.621 ± 0.321
0.171MetCys: 0.171 ± 0.114
0.768MetAsp: 0.768 ± 0.223
1.536MetGlu: 1.536 ± 0.298
1.365MetPhe: 1.365 ± 0.265
1.024MetGly: 1.024 ± 0.31
0.341MetHis: 0.341 ± 0.162
1.365MetIle: 1.365 ± 0.31
2.304MetLys: 2.304 ± 0.455
1.792MetLeu: 1.792 ± 0.326
0.171MetMet: 0.171 ± 0.116
1.109MetAsn: 1.109 ± 0.311
0.853MetPro: 0.853 ± 0.217
1.45MetGln: 1.45 ± 0.334
1.109MetArg: 1.109 ± 0.242
1.706MetSer: 1.706 ± 0.359
1.109MetThr: 1.109 ± 0.253
0.853MetVal: 0.853 ± 0.206
0.256MetTrp: 0.256 ± 0.13
0.341MetTyr: 0.341 ± 0.171
0.0MetXaa: 0.0 ± 0.0
Asn
4.692AsnAla: 4.692 ± 0.632
0.597AsnCys: 0.597 ± 0.231
4.607AsnAsp: 4.607 ± 0.742
6.399AsnGlu: 6.399 ± 0.745
4.692AsnPhe: 4.692 ± 0.789
4.181AsnGly: 4.181 ± 0.567
1.109AsnHis: 1.109 ± 0.261
5.546AsnIle: 5.546 ± 0.591
8.19AsnLys: 8.19 ± 1.332
7.849AsnLeu: 7.849 ± 0.79
0.853AsnMet: 0.853 ± 0.246
5.204AsnAsn: 5.204 ± 0.936
2.474AsnPro: 2.474 ± 0.45
2.645AsnGln: 2.645 ± 0.469
2.133AsnArg: 2.133 ± 0.39
5.716AsnSer: 5.716 ± 0.641
4.778AsnThr: 4.778 ± 0.556
5.119AsnVal: 5.119 ± 0.477
0.683AsnTrp: 0.683 ± 0.252
4.181AsnTyr: 4.181 ± 0.556
0.0AsnXaa: 0.0 ± 0.0
Pro
1.109ProAla: 1.109 ± 0.287
0.427ProCys: 0.427 ± 0.157
0.768ProAsp: 0.768 ± 0.251
1.706ProGlu: 1.706 ± 0.453
1.877ProPhe: 1.877 ± 0.444
0.0ProGly: 0.0 ± 0.0
0.171ProHis: 0.171 ± 0.116
1.621ProIle: 1.621 ± 0.407
1.962ProLys: 1.962 ± 0.45
2.645ProLeu: 2.645 ± 0.507
0.597ProMet: 0.597 ± 0.208
2.474ProAsn: 2.474 ± 0.396
0.256ProPro: 0.256 ± 0.179
1.28ProGln: 1.28 ± 0.28
0.768ProArg: 0.768 ± 0.258
2.133ProSer: 2.133 ± 0.433
1.792ProThr: 1.792 ± 0.487
1.194ProVal: 1.194 ± 0.301
0.0ProTrp: 0.0 ± 0.0
1.45ProTyr: 1.45 ± 0.455
0.0ProXaa: 0.0 ± 0.0
Gln
2.133GlnAla: 2.133 ± 0.635
0.341GlnCys: 0.341 ± 0.174
1.28GlnAsp: 1.28 ± 0.286
2.133GlnGlu: 2.133 ± 0.483
1.28GlnPhe: 1.28 ± 0.297
2.304GlnGly: 2.304 ± 0.4
0.853GlnHis: 0.853 ± 0.328
4.095GlnIle: 4.095 ± 0.548
4.095GlnLys: 4.095 ± 0.684
3.413GlnLeu: 3.413 ± 0.49
1.194GlnMet: 1.194 ± 0.417
2.389GlnAsn: 2.389 ± 0.427
1.365GlnPro: 1.365 ± 0.394
2.133GlnGln: 2.133 ± 0.722
1.962GlnArg: 1.962 ± 0.458
2.474GlnSer: 2.474 ± 0.466
2.218GlnThr: 2.218 ± 0.559
1.621GlnVal: 1.621 ± 0.39
0.427GlnTrp: 0.427 ± 0.171
1.536GlnTyr: 1.536 ± 0.348
0.0GlnXaa: 0.0 ± 0.0
Arg
0.938ArgAla: 0.938 ± 0.34
0.427ArgCys: 0.427 ± 0.151
1.365ArgAsp: 1.365 ± 0.324
2.56ArgGlu: 2.56 ± 0.591
1.024ArgPhe: 1.024 ± 0.274
1.194ArgGly: 1.194 ± 0.389
0.427ArgHis: 0.427 ± 0.211
2.56ArgIle: 2.56 ± 0.523
3.413ArgLys: 3.413 ± 0.519
3.242ArgLeu: 3.242 ± 0.555
0.768ArgMet: 0.768 ± 0.223
1.877ArgAsn: 1.877 ± 0.342
0.171ArgPro: 0.171 ± 0.113
0.938ArgGln: 0.938 ± 0.316
1.109ArgArg: 1.109 ± 0.268
2.133ArgSer: 2.133 ± 0.44
2.133ArgThr: 2.133 ± 0.399
2.218ArgVal: 2.218 ± 0.345
0.171ArgTrp: 0.171 ± 0.12
1.792ArgTyr: 1.792 ± 0.369
0.0ArgXaa: 0.0 ± 0.0
Ser
2.304SerAla: 2.304 ± 0.427
0.853SerCys: 0.853 ± 0.28
4.181SerAsp: 4.181 ± 0.715
4.948SerGlu: 4.948 ± 0.764
4.351SerPhe: 4.351 ± 0.681
3.925SerGly: 3.925 ± 0.68
0.597SerHis: 0.597 ± 0.202
6.399SerIle: 6.399 ± 0.629
5.546SerLys: 5.546 ± 0.733
5.802SerLeu: 5.802 ± 0.738
1.109SerMet: 1.109 ± 0.289
5.631SerAsn: 5.631 ± 0.819
1.45SerPro: 1.45 ± 0.318
2.474SerGln: 2.474 ± 0.52
1.621SerArg: 1.621 ± 0.351
4.01SerSer: 4.01 ± 0.605
2.901SerThr: 2.901 ± 0.507
4.436SerVal: 4.436 ± 0.575
0.341SerTrp: 0.341 ± 0.202
2.133SerTyr: 2.133 ± 0.452
0.0SerXaa: 0.0 ± 0.0
Thr
4.266ThrAla: 4.266 ± 0.693
0.597ThrCys: 0.597 ± 0.2
4.436ThrAsp: 4.436 ± 0.61
4.522ThrGlu: 4.522 ± 0.727
4.266ThrPhe: 4.266 ± 0.622
3.583ThrGly: 3.583 ± 0.653
0.938ThrHis: 0.938 ± 0.234
5.972ThrIle: 5.972 ± 0.971
5.204ThrLys: 5.204 ± 0.52
6.313ThrLeu: 6.313 ± 0.682
1.28ThrMet: 1.28 ± 0.337
4.778ThrAsn: 4.778 ± 0.704
1.621ThrPro: 1.621 ± 0.371
3.071ThrGln: 3.071 ± 0.58
1.792ThrArg: 1.792 ± 0.414
3.669ThrSer: 3.669 ± 0.661
5.375ThrThr: 5.375 ± 0.967
1.621ThrVal: 1.621 ± 0.398
0.768ThrTrp: 0.768 ± 0.245
2.304ThrTyr: 2.304 ± 0.486
0.0ThrXaa: 0.0 ± 0.0
Val
3.327ValAla: 3.327 ± 0.482
0.683ValCys: 0.683 ± 0.244
2.645ValAsp: 2.645 ± 0.464
2.901ValGlu: 2.901 ± 0.579
3.071ValPhe: 3.071 ± 0.443
2.73ValGly: 2.73 ± 0.396
0.597ValHis: 0.597 ± 0.226
3.839ValIle: 3.839 ± 0.451
5.375ValLys: 5.375 ± 0.557
4.778ValLeu: 4.778 ± 0.513
1.194ValMet: 1.194 ± 0.261
4.948ValAsn: 4.948 ± 0.824
1.024ValPro: 1.024 ± 0.26
2.218ValGln: 2.218 ± 0.407
1.877ValArg: 1.877 ± 0.494
4.095ValSer: 4.095 ± 0.617
2.389ValThr: 2.389 ± 0.496
2.901ValVal: 2.901 ± 0.556
0.683ValTrp: 0.683 ± 0.243
2.048ValTyr: 2.048 ± 0.33
0.0ValXaa: 0.0 ± 0.0
Trp
0.512TrpAla: 0.512 ± 0.195
0.085TrpCys: 0.085 ± 0.082
0.768TrpAsp: 0.768 ± 0.236
0.512TrpGlu: 0.512 ± 0.19
0.256TrpPhe: 0.256 ± 0.139
0.256TrpGly: 0.256 ± 0.139
0.256TrpHis: 0.256 ± 0.147
0.768TrpIle: 0.768 ± 0.258
1.194TrpLys: 1.194 ± 0.409
1.109TrpLeu: 1.109 ± 0.238
0.256TrpMet: 0.256 ± 0.124
0.768TrpAsn: 0.768 ± 0.355
0.0TrpPro: 0.0 ± 0.0
0.256TrpGln: 0.256 ± 0.132
0.512TrpArg: 0.512 ± 0.235
0.256TrpSer: 0.256 ± 0.18
0.512TrpThr: 0.512 ± 0.183
0.256TrpVal: 0.256 ± 0.143
0.0TrpTrp: 0.0 ± 0.0
0.427TrpTyr: 0.427 ± 0.22
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.706TyrAla: 1.706 ± 0.286
0.938TyrCys: 0.938 ± 0.28
2.389TyrAsp: 2.389 ± 0.434
2.986TyrGlu: 2.986 ± 0.492
1.706TyrPhe: 1.706 ± 0.391
1.962TyrGly: 1.962 ± 0.395
0.512TyrHis: 0.512 ± 0.185
3.925TyrIle: 3.925 ± 0.671
4.436TyrLys: 4.436 ± 0.811
3.669TyrLeu: 3.669 ± 0.535
0.427TyrMet: 0.427 ± 0.167
4.266TyrAsn: 4.266 ± 0.612
1.194TyrPro: 1.194 ± 0.373
1.28TyrGln: 1.28 ± 0.322
1.365TyrArg: 1.365 ± 0.379
2.56TyrSer: 2.56 ± 0.428
2.901TyrThr: 2.901 ± 0.478
2.048TyrVal: 2.048 ± 0.47
0.341TyrTrp: 0.341 ± 0.127
2.218TyrTyr: 2.218 ± 0.526
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (11722 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski