Escherichia phage phiEcoM-GJ1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Chaseviridae; Cleopatravirinae; Carltongylesvirus; Escherichia virus GJ1

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9Q1S6|A9Q1S6_9CAUD Hypothetical phage protein OS=Escherichia phage phiEcoM-GJ1 OX=451705 PE=4 SV=1
MM1 pKa = 7.75EE2 pKa = 4.47NQIIQAVTQLKK13 pKa = 9.06SWRR16 pKa = 11.84RR17 pKa = 11.84DD18 pKa = 2.9EE19 pKa = 4.16TLLVRR24 pKa = 11.84FYY26 pKa = 10.75TGEE29 pKa = 4.04NNSKK33 pKa = 10.84FEE35 pKa = 4.39DD36 pKa = 3.44VWIDD40 pKa = 3.43GLSWRR45 pKa = 11.84LYY47 pKa = 10.38DD48 pKa = 4.62FDD50 pKa = 3.54MSEE53 pKa = 4.49YY54 pKa = 10.57LHH56 pKa = 6.94EE57 pKa = 5.81DD58 pKa = 3.15YY59 pKa = 11.15GIEE62 pKa = 4.07YY63 pKa = 9.58PEE65 pKa = 4.37TGTYY69 pKa = 10.26DD70 pKa = 3.1ILEE73 pKa = 4.3SSEE76 pKa = 4.39ADD78 pKa = 3.32VTEE81 pKa = 4.06VCFVDD86 pKa = 3.83GLFSIRR92 pKa = 11.84RR93 pKa = 11.84YY94 pKa = 11.02DD95 pKa = 3.46KK96 pKa = 11.01FNEE99 pKa = 4.09LLTDD103 pKa = 3.45TDD105 pKa = 4.41LDD107 pKa = 4.08IILAAFRR114 pKa = 11.84SDD116 pKa = 3.79EE117 pKa = 3.86IDD119 pKa = 2.96IFTVYY124 pKa = 10.79DD125 pKa = 4.16LNDD128 pKa = 3.52MFITRR133 pKa = 11.84CDD135 pKa = 3.76SIVDD139 pKa = 3.62YY140 pKa = 10.46FKK142 pKa = 10.89QDD144 pKa = 3.3DD145 pKa = 4.76GIEE148 pKa = 4.21VPEE151 pKa = 4.1NLRR154 pKa = 11.84PYY156 pKa = 10.25IDD158 pKa = 3.33WDD160 pKa = 4.32AIARR164 pKa = 11.84DD165 pKa = 3.89YY166 pKa = 11.6GSDD169 pKa = 3.69YY170 pKa = 10.94IFSEE174 pKa = 4.64GVLFRR179 pKa = 11.84NAA181 pKa = 3.4

Molecular weight: 21.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9Q1S8|A9Q1S8_9CAUD Hypothetical phage protein OS=Escherichia phage phiEcoM-GJ1 OX=451705 PE=4 SV=1
MM1 pKa = 7.51IVTPIKK7 pKa = 10.37VKK9 pKa = 10.43KK10 pKa = 10.35SKK12 pKa = 10.8LSLQEE17 pKa = 3.41RR18 pKa = 11.84TLRR21 pKa = 11.84STIRR25 pKa = 11.84GITANRR31 pKa = 11.84ISSGDD36 pKa = 3.46IQAKK40 pKa = 7.79PCRR43 pKa = 11.84VCGSTFVEE51 pKa = 4.24AHH53 pKa = 5.42HH54 pKa = 6.59WDD56 pKa = 3.18YY57 pKa = 10.81RR58 pKa = 11.84TYY60 pKa = 11.17KK61 pKa = 10.83VIFLCAKK68 pKa = 10.23HH69 pKa = 5.99HH70 pKa = 5.66VEE72 pKa = 3.06WHH74 pKa = 4.46NRR76 pKa = 11.84QRR78 pKa = 11.84YY79 pKa = 8.53FEE81 pKa = 4.69HH82 pKa = 7.2LNTAYY87 pKa = 10.38GIKK90 pKa = 9.83FVHH93 pKa = 6.4RR94 pKa = 11.84PKK96 pKa = 10.43PFRR99 pKa = 11.84FKK101 pKa = 10.02WVWVKK106 pKa = 10.62NHH108 pKa = 5.51MGYY111 pKa = 10.12RR112 pKa = 11.84PRR114 pKa = 11.84PSEE117 pKa = 3.86TMPSVIRR124 pKa = 11.84RR125 pKa = 11.84GQKK128 pKa = 9.67LIKK131 pKa = 10.47VII133 pKa = 4.08

Molecular weight: 15.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

15645

39

1239

208.6

23.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.265 ± 0.45

1.093 ± 0.146

5.88 ± 0.266

6.232 ± 0.282

4.033 ± 0.205

7.37 ± 0.382

2.026 ± 0.203

6.155 ± 0.25

6.13 ± 0.382

7.689 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.851 ± 0.186

4.986 ± 0.208

3.758 ± 0.166

3.835 ± 0.213

4.794 ± 0.224

6.878 ± 0.362

6.098 ± 0.335

6.91 ± 0.298

1.381 ± 0.119

3.637 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski