Fictibacillus macauensis ZFHKF-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Fictibacillus; Fictibacillus macauensis

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3850 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I8UJX2|I8UJX2_9BACI Flavin_Reduct domain-containing protein OS=Fictibacillus macauensis ZFHKF-1 OX=1196324 GN=A374_02679 PE=4 SV=1
MM1 pKa = 7.61AFSLSEE7 pKa = 4.28SVISLYY13 pKa = 10.63RR14 pKa = 11.84QIDD17 pKa = 3.69FDD19 pKa = 4.06EE20 pKa = 6.12GEE22 pKa = 4.18ALQLFARR29 pKa = 11.84YY30 pKa = 9.46SGSPDD35 pKa = 2.93HH36 pKa = 6.86GGYY39 pKa = 10.7AIGVQKK45 pKa = 10.31GHH47 pKa = 6.7PTDD50 pKa = 3.25NDD52 pKa = 3.82YY53 pKa = 11.08IQEE56 pKa = 4.26YY57 pKa = 10.16EE58 pKa = 4.09GLTVFVRR65 pKa = 11.84PDD67 pKa = 3.62DD68 pKa = 3.44QWFVQDD74 pKa = 4.46MMLDD78 pKa = 3.51YY79 pKa = 11.23DD80 pKa = 3.82EE81 pKa = 6.0QEE83 pKa = 4.42DD84 pKa = 4.25FFSFEE89 pKa = 4.05FPAVLL94 pKa = 4.27

Molecular weight: 10.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I8AKD1|I8AKD1_9BACI Uncharacterized protein OS=Fictibacillus macauensis ZFHKF-1 OX=1196324 GN=A374_09239 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.74PTFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.45GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3850

0

3850

1063458

26

1518

276.2

30.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.762 ± 0.041

0.806 ± 0.011

4.678 ± 0.033

6.968 ± 0.056

4.369 ± 0.036

6.824 ± 0.042

2.509 ± 0.027

6.906 ± 0.036

6.684 ± 0.05

10.087 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.842 ± 0.017

3.787 ± 0.025

3.657 ± 0.027

4.154 ± 0.027

4.29 ± 0.034

6.09 ± 0.03

5.743 ± 0.03

7.23 ± 0.033

1.042 ± 0.016

3.571 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski