Torque teno douroucouli virus (isolate At-TTV3)

Taxonomy: Viruses; Anelloviridae; Zetatorquevirus; Torque teno douroucouli virus

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9DUB8|ORF2_TTVZ1 Uncharacterized ORF2 protein OS=Torque teno douroucouli virus (isolate At-TTV3) OX=766187 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.77GDD3 pKa = 2.95WFMQGSPPDD12 pKa = 3.51FAVQEE17 pKa = 4.22DD18 pKa = 3.96RR19 pKa = 11.84WLKK22 pKa = 10.58AVEE25 pKa = 4.29SCHH28 pKa = 7.01QLFCSCSSAWDD39 pKa = 3.4HH40 pKa = 6.52LRR42 pKa = 11.84NILQTRR48 pKa = 11.84GYY50 pKa = 8.51WPQGPPPYY58 pKa = 9.54RR59 pKa = 11.84SEE61 pKa = 3.68PHH63 pKa = 6.21TEE65 pKa = 3.92HH66 pKa = 6.92SRR68 pKa = 11.84PPPPKK73 pKa = 9.75KK74 pKa = 9.76RR75 pKa = 11.84RR76 pKa = 11.84PWCGGDD82 pKa = 3.12GGGDD86 pKa = 3.17AGAGPSGVAGTAAGGAGGDD105 pKa = 3.84GAVGPVEE112 pKa = 4.65EE113 pKa = 5.73DD114 pKa = 3.62DD115 pKa = 3.9PTVADD120 pKa = 5.52LIAAMEE126 pKa = 4.13EE127 pKa = 4.35DD128 pKa = 3.88EE129 pKa = 4.46RR130 pKa = 5.85

Molecular weight: 13.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9DUB8|ORF2_TTVZ1 Uncharacterized ORF2 protein OS=Torque teno douroucouli virus (isolate At-TTV3) OX=766187 GN=ORF2 PE=4 SV=1
MM1 pKa = 6.46VWRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84WWRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84PVWRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84YY20 pKa = 9.1RR21 pKa = 11.84RR22 pKa = 11.84WRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84WRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84PRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84PYY40 pKa = 9.18RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84PYY45 pKa = 9.76RR46 pKa = 11.84RR47 pKa = 11.84YY48 pKa = 8.74GRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84KK53 pKa = 8.93VRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84TLVVRR65 pKa = 11.84QFQPPYY71 pKa = 9.76QRR73 pKa = 11.84RR74 pKa = 11.84CRR76 pKa = 11.84VSGLEE81 pKa = 3.63PLLYY85 pKa = 10.19FGKK88 pKa = 10.34KK89 pKa = 8.03EE90 pKa = 4.18EE91 pKa = 4.1EE92 pKa = 3.63RR93 pKa = 11.84HH94 pKa = 5.64FYY96 pKa = 11.1GNRR99 pKa = 11.84QIMKK103 pKa = 10.34DD104 pKa = 3.18VDD106 pKa = 3.54QTEE109 pKa = 4.6GGGVTDD115 pKa = 4.68GVFSLEE121 pKa = 3.97EE122 pKa = 4.28LYY124 pKa = 10.86EE125 pKa = 4.39SYY127 pKa = 10.84QEE129 pKa = 4.72GWNHH133 pKa = 5.56WSASNQMLPLVRR145 pKa = 11.84YY146 pKa = 7.36FGCKK150 pKa = 6.61ITLYY154 pKa = 10.64RR155 pKa = 11.84SATDD159 pKa = 4.67SYY161 pKa = 11.13IFWWTNRR168 pKa = 11.84PPFQVDD174 pKa = 3.18RR175 pKa = 11.84QFWVGMHH182 pKa = 6.97PYY184 pKa = 9.51WMLRR188 pKa = 11.84RR189 pKa = 11.84GRR191 pKa = 11.84KK192 pKa = 9.01VVMLSKK198 pKa = 10.23QLKK201 pKa = 9.35PFAKK205 pKa = 10.27DD206 pKa = 2.89HH207 pKa = 4.87VTIKK211 pKa = 10.25IGPPALMKK219 pKa = 9.03TKK221 pKa = 9.6WYY223 pKa = 9.15FARR226 pKa = 11.84DD227 pKa = 3.73FCKK230 pKa = 9.78TWLLQWRR237 pKa = 11.84ACFFNPIDD245 pKa = 3.95PYY247 pKa = 10.71IPRR250 pKa = 11.84GANNTTVTFYY260 pKa = 11.13GFPGHH265 pKa = 6.7SYY267 pKa = 10.34PSQSLYY273 pKa = 9.04YY274 pKa = 9.22TWQEE278 pKa = 3.82HH279 pKa = 5.23LAEE282 pKa = 4.34TNVWGITNEE291 pKa = 3.98SEE293 pKa = 4.09KK294 pKa = 10.61TKK296 pKa = 10.99FEE298 pKa = 4.42ALRR301 pKa = 11.84KK302 pKa = 9.15AWNEE306 pKa = 3.72QATKK310 pKa = 10.89GPVAGYY316 pKa = 10.05KK317 pKa = 10.23AVLQAMNAWASVAAPGPEE335 pKa = 3.45GSNFAKK341 pKa = 8.95GTNVALGLANLLWLIYY357 pKa = 9.83SQYY360 pKa = 9.24YY361 pKa = 6.56WQNRR365 pKa = 11.84SPSPWGLPGATLEE378 pKa = 4.36AMKK381 pKa = 10.51HH382 pKa = 4.74PEE384 pKa = 3.29AWKK387 pKa = 9.48PYY389 pKa = 7.98AYY391 pKa = 9.6KK392 pKa = 10.81YY393 pKa = 10.52SWSHH397 pKa = 7.24DD398 pKa = 3.25KK399 pKa = 11.56GKK401 pKa = 11.02GNRR404 pKa = 11.84FAICDD409 pKa = 3.61WPYY412 pKa = 11.16KK413 pKa = 9.47DD414 pKa = 3.41QHH416 pKa = 7.08GEE418 pKa = 3.63QTTPLNFDD426 pKa = 4.84LEE428 pKa = 4.56IKK430 pKa = 10.19DD431 pKa = 4.66APLWWCVTAAYY442 pKa = 9.99DD443 pKa = 3.23WAIRR447 pKa = 11.84QGKK450 pKa = 8.36NPANWSFFVRR460 pKa = 11.84SDD462 pKa = 3.81YY463 pKa = 10.12TWPRR467 pKa = 11.84ADD469 pKa = 4.64NIILAYY475 pKa = 10.08SSYY478 pKa = 10.77FADD481 pKa = 2.87NVVKK485 pKa = 10.69NKK487 pKa = 10.4YY488 pKa = 10.12ADD490 pKa = 3.8PQHH493 pKa = 6.1ATGGYY498 pKa = 5.97FTLYY502 pKa = 10.14RR503 pKa = 11.84APNLIDD509 pKa = 3.31GRR511 pKa = 11.84EE512 pKa = 4.31AIDD515 pKa = 3.4IIYY518 pKa = 10.32RR519 pKa = 11.84NGPFAPKK526 pKa = 10.04QEE528 pKa = 4.14EE529 pKa = 4.46STGNATIKK537 pKa = 10.31YY538 pKa = 9.02RR539 pKa = 11.84FYY541 pKa = 10.8FKK543 pKa = 10.18WGGTQRR549 pKa = 11.84PPRR552 pKa = 11.84PQPTDD557 pKa = 3.13PCQQPRR563 pKa = 11.84WEE565 pKa = 4.94GPNFPPFDD573 pKa = 3.15GRR575 pKa = 11.84ARR577 pKa = 11.84IDD579 pKa = 3.59NPSDD583 pKa = 3.25QDD585 pKa = 3.33PRR587 pKa = 11.84SYY589 pKa = 10.76FWPFDD594 pKa = 3.48TRR596 pKa = 11.84RR597 pKa = 11.84GSLTASAVDD606 pKa = 3.48RR607 pKa = 11.84VKK609 pKa = 10.9RR610 pKa = 11.84SLDD613 pKa = 3.2AVTEE617 pKa = 4.08PSEE620 pKa = 3.97TRR622 pKa = 11.84LRR624 pKa = 11.84EE625 pKa = 4.12GEE627 pKa = 4.16EE628 pKa = 4.75TYY630 pKa = 11.25GLPPPEE636 pKa = 4.47KK637 pKa = 8.65RR638 pKa = 11.84ARR640 pKa = 11.84WGFPSILPTEE650 pKa = 4.36NPTDD654 pKa = 3.44EE655 pKa = 4.34DD656 pKa = 3.33EE657 pKa = 5.36RR658 pKa = 11.84YY659 pKa = 10.52LEE661 pKa = 4.67AEE663 pKa = 4.42ADD665 pKa = 3.86TSSSDD670 pKa = 3.31ATDD673 pKa = 4.14GVPEE677 pKa = 4.39TPFEE681 pKa = 4.17LLQHH685 pKa = 5.87IQRR688 pKa = 11.84DD689 pKa = 3.41RR690 pKa = 11.84DD691 pKa = 3.16KK692 pKa = 11.15RR693 pKa = 11.84RR694 pKa = 11.84EE695 pKa = 3.9LRR697 pKa = 11.84GRR699 pKa = 11.84LARR702 pKa = 11.84LILRR706 pKa = 11.84LRR708 pKa = 11.84RR709 pKa = 11.84RR710 pKa = 11.84QDD712 pKa = 3.28PEE714 pKa = 3.8LQNYY718 pKa = 8.29YY719 pKa = 10.15PP720 pKa = 4.41

Molecular weight: 85.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1001

130

720

333.7

38.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.293 ± 0.814

1.299 ± 0.538

5.594 ± 0.91

5.095 ± 0.787

3.596 ± 1.025

7.293 ± 2.133

1.598 ± 0.408

2.797 ± 0.513

4.196 ± 0.839

5.994 ± 0.651

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.399 ± 0.266

3.097 ± 1.151

8.991 ± 1.031

3.796 ± 0.665

12.787 ± 1.941

6.893 ± 2.897

6.194 ± 1.582

3.696 ± 0.583

3.996 ± 0.926

4.396 ± 1.744
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski