Bos mutus (wild yak)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria;

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L8IK42|L8IK42_9CETA Inter-alpha-trypsin inhibitor heavy chain H4 OS=Bos mutus OX=72004 GN=M91_14526 PE=3 SV=1
PP1 pKa = 5.8QTAFAPADD9 pKa = 3.35HH10 pKa = 6.86SSQEE14 pKa = 4.14KK15 pKa = 8.84ATEE18 pKa = 3.96NATDD22 pKa = 4.09RR23 pKa = 11.84LNGAQSIPHH32 pKa = 7.22DD33 pKa = 4.34SPAHH37 pKa = 5.9GEE39 pKa = 4.19GTHH42 pKa = 6.63CEE44 pKa = 4.09EE45 pKa = 4.67EE46 pKa = 4.83GFAEE50 pKa = 5.34DD51 pKa = 6.15DD52 pKa = 3.82EE53 pKa = 7.08DD54 pKa = 5.82SDD56 pKa = 5.27GEE58 pKa = 4.03PSPWEE63 pKa = 3.86LSEE66 pKa = 4.68GMSGCLPKK74 pKa = 10.57EE75 pKa = 3.68QAGDD79 pKa = 3.86LFHH82 pKa = 7.39EE83 pKa = 4.81DD84 pKa = 2.66WDD86 pKa = 4.61LEE88 pKa = 4.33LKK90 pKa = 10.49ADD92 pKa = 3.94QGNPYY97 pKa = 10.37DD98 pKa = 4.73ADD100 pKa = 5.11DD101 pKa = 3.93IQGCLSQEE109 pKa = 3.69VRR111 pKa = 11.84PWVCCAPQGDD121 pKa = 4.66MIYY124 pKa = 10.59DD125 pKa = 4.29PSWHH129 pKa = 6.9HH130 pKa = 6.75PPPLIPHH137 pKa = 5.6YY138 pKa = 10.76SKK140 pKa = 10.49MVFEE144 pKa = 5.05TGQFDD149 pKa = 4.21DD150 pKa = 6.01AEE152 pKa = 4.35DD153 pKa = 3.31

Molecular weight: 16.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L8HSA8|L8HSA8_9CETA Olfactory receptor (Fragment) OS=Bos mutus OX=72004 GN=M91_10411 PE=3 SV=1
PP1 pKa = 7.81RR2 pKa = 11.84AQSSQVQSPQAQSSQVGSPRR22 pKa = 11.84GQSSQVPSPRR32 pKa = 11.84GQSSQIRR39 pKa = 11.84SPRR42 pKa = 11.84AQSSQVRR49 pKa = 11.84SPQAQLSQVRR59 pKa = 11.84SPRR62 pKa = 11.84AQSSQVRR69 pKa = 11.84SPRR72 pKa = 11.84AQSSQVGSPQGQSSQVWSPRR92 pKa = 11.84TQSSQVGSPQGQSSQVPSPRR112 pKa = 11.84AQSSQVGSPRR122 pKa = 11.84AQSSQVPSPWGQSSQVV138 pKa = 3.03

Molecular weight: 14.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18544

9

18553

8861750

30

7294

477.6

53.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.85 ± 0.019

2.293 ± 0.016

4.723 ± 0.012

6.901 ± 0.026

3.858 ± 0.013

6.405 ± 0.026

2.607 ± 0.011

4.567 ± 0.018

5.759 ± 0.024

10.192 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.146 ± 0.009

3.588 ± 0.014

6.112 ± 0.024

4.637 ± 0.016

5.585 ± 0.016

8.259 ± 0.024

5.339 ± 0.014

6.177 ± 0.015

1.235 ± 0.008

2.763 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski