Sphingomonas sp. Leaf357

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3524 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5QXU7|A0A0Q5QXU7_9SPHN PEP-CTERM domain-containing protein OS=Sphingomonas sp. Leaf357 OX=1736350 GN=ASG11_07965 PE=4 SV=1
MM1 pKa = 7.2NLAGVSYY8 pKa = 11.02YY9 pKa = 9.06GTQYY13 pKa = 11.15PFLDD17 pKa = 3.38RR18 pKa = 11.84FKK20 pKa = 10.64TSSEE24 pKa = 3.56WMVQGVNGQPIGALSLDD41 pKa = 3.48ANGYY45 pKa = 5.06PTAMPTGATNIFTMVGLDD63 pKa = 3.6PVALEE68 pKa = 4.02TSDD71 pKa = 4.1IYY73 pKa = 11.53VITYY77 pKa = 9.47KK78 pKa = 10.99GNASINILGGTILSSEE94 pKa = 4.15PGKK97 pKa = 9.24ITFRR101 pKa = 11.84FTGEE105 pKa = 4.03GSSTAINVSAIDD117 pKa = 3.73PANPLSDD124 pKa = 3.36MHH126 pKa = 6.97VVRR129 pKa = 11.84QDD131 pKa = 3.17QVALYY136 pKa = 10.03EE137 pKa = 4.33SGEE140 pKa = 4.07IFNPAFLDD148 pKa = 4.24KK149 pKa = 10.82ASQWSEE155 pKa = 3.69LRR157 pKa = 11.84YY158 pKa = 9.41MDD160 pKa = 4.85WGNTNNNTLTSWDD173 pKa = 3.42QRR175 pKa = 11.84TTLTSASWSNISSTHH190 pKa = 5.03SVPIEE195 pKa = 3.92IMVALANKK203 pKa = 8.67TNTDD207 pKa = 2.64MWINIPAAADD217 pKa = 3.23DD218 pKa = 4.23DD219 pKa = 4.98YY220 pKa = 11.7IRR222 pKa = 11.84KK223 pKa = 6.93TLSYY227 pKa = 11.14VRR229 pKa = 11.84DD230 pKa = 3.56NLAAGLSVKK239 pKa = 10.15VEE241 pKa = 4.02YY242 pKa = 11.14SNEE245 pKa = 3.31VWNWGFQQASYY256 pKa = 9.37AQTMGAQMFGTDD268 pKa = 4.09LNGNGQIDD276 pKa = 3.74RR277 pKa = 11.84GTSEE281 pKa = 4.43EE282 pKa = 4.57PGTAYY287 pKa = 10.33LQYY290 pKa = 9.98YY291 pKa = 8.72GYY293 pKa = 10.67RR294 pKa = 11.84AAQVAAVANSVFASSDD310 pKa = 3.29PARR313 pKa = 11.84LQNVLSTQTGYY324 pKa = 11.29LGLEE328 pKa = 4.02TYY330 pKa = 10.45IFDD333 pKa = 4.43GVQSAALGSVASLFDD348 pKa = 3.41NYY350 pKa = 11.28AITTYY355 pKa = 10.67FGLDD359 pKa = 3.23SVDD362 pKa = 3.39QATLVSWAHH371 pKa = 6.55AGQAGIDD378 pKa = 3.45AAFAGLNASLNDD390 pKa = 3.5SLRR393 pKa = 11.84VYY395 pKa = 10.5VYY397 pKa = 9.57QQAVAARR404 pKa = 11.84NGLSLVAYY412 pKa = 9.08EE413 pKa = 4.67GGVGLAAYY421 pKa = 7.47TFADD425 pKa = 3.79ADD427 pKa = 3.71QSAMLAFFEE436 pKa = 5.58ALDD439 pKa = 3.81NDD441 pKa = 3.61PRR443 pKa = 11.84MGDD446 pKa = 3.23MYY448 pKa = 11.65AKK450 pKa = 9.72MVADD454 pKa = 5.34FAASGGSGLNAFTDD468 pKa = 3.59AGSPSKK474 pKa = 10.22WGTWGTLDD482 pKa = 4.26SIYY485 pKa = 10.91DD486 pKa = 3.89EE487 pKa = 4.72GSEE490 pKa = 4.73RR491 pKa = 11.84YY492 pKa = 9.03DD493 pKa = 3.48ALVAASQAAKK503 pKa = 10.12AAQLTGGDD511 pKa = 3.45ATQVNVLTSAASYY524 pKa = 11.08VLGGNQLTLTYY535 pKa = 10.3VGSGGFNGTGNDD547 pKa = 3.59LSNTIVGGNSFNMLYY562 pKa = 10.76GGGGDD567 pKa = 3.64DD568 pKa = 4.14TIVGGSRR575 pKa = 11.84NDD577 pKa = 3.64IIDD580 pKa = 3.86GGTGADD586 pKa = 3.53RR587 pKa = 11.84MIGGDD592 pKa = 3.58GDD594 pKa = 3.69DD595 pKa = 4.13TYY597 pKa = 12.03VVDD600 pKa = 4.54NIGDD604 pKa = 3.92VVVEE608 pKa = 4.0QAGGGTDD615 pKa = 3.52EE616 pKa = 5.5VRR618 pKa = 11.84TTLNTYY624 pKa = 10.92ALTASVEE631 pKa = 4.01NLTFIGTGVITGTGNDD647 pKa = 3.52SDD649 pKa = 4.84NIIAAVAGASASLYY663 pKa = 10.76GGAGNDD669 pKa = 3.57TLTGSTGADD678 pKa = 3.41LLDD681 pKa = 4.49GGTGADD687 pKa = 2.96IMRR690 pKa = 11.84GGRR693 pKa = 11.84GNDD696 pKa = 3.25VYY698 pKa = 11.04IVDD701 pKa = 3.77NAGDD705 pKa = 3.96NVIEE709 pKa = 4.12LAGGGIDD716 pKa = 4.05TVRR719 pKa = 11.84TTLNLYY725 pKa = 10.0GLASEE730 pKa = 4.7VEE732 pKa = 4.17NLTFIGTGNFNAFGNSLDD750 pKa = 3.7NVMTGGAGDD759 pKa = 3.62DD760 pKa = 4.13TLWGDD765 pKa = 4.23VGNDD769 pKa = 3.65TLNGGAGNDD778 pKa = 3.59VLIGGVGADD787 pKa = 3.63TMIGGLGDD795 pKa = 3.99DD796 pKa = 4.97RR797 pKa = 11.84YY798 pKa = 11.31DD799 pKa = 3.23VDD801 pKa = 4.27NIGDD805 pKa = 3.9IVVEE809 pKa = 4.76LPGQGNDD816 pKa = 3.42AVYY819 pKa = 8.13TTVNYY824 pKa = 9.19TLTANVEE831 pKa = 4.06NLMLSGTGNINGTGNEE847 pKa = 4.52LNNIMFGNSGDD858 pKa = 4.18NILSGGAGDD867 pKa = 4.03DD868 pKa = 3.83TLIGGAGNDD877 pKa = 3.8TLVGGDD883 pKa = 4.41GNDD886 pKa = 3.66SLDD889 pKa = 4.17GGDD892 pKa = 5.46GDD894 pKa = 6.07DD895 pKa = 4.07ILNGRR900 pKa = 11.84MGDD903 pKa = 3.76DD904 pKa = 5.63LITGGAGNDD913 pKa = 4.03VIWGGIGRR921 pKa = 11.84DD922 pKa = 3.76VMDD925 pKa = 5.1GGAGADD931 pKa = 3.16RR932 pKa = 11.84FIFYY936 pKa = 9.96TGDD939 pKa = 3.33TGKK942 pKa = 8.16TRR944 pKa = 11.84ATADD948 pKa = 3.36MIYY951 pKa = 10.31FSHH954 pKa = 7.4SDD956 pKa = 3.12GDD958 pKa = 4.56LIDD961 pKa = 4.63LSRR964 pKa = 11.84MDD966 pKa = 4.18ARR968 pKa = 11.84TATSVDD974 pKa = 3.51DD975 pKa = 4.07AFSFIGQAAFTNTAGEE991 pKa = 4.08LRR993 pKa = 11.84IQYY996 pKa = 10.35SGGYY1000 pKa = 8.54WDD1002 pKa = 5.23VLGDD1006 pKa = 3.76TNGDD1010 pKa = 3.88GIADD1014 pKa = 3.95FSITVSAGSTPLIARR1029 pKa = 11.84DD1030 pKa = 3.76FVLL1033 pKa = 5.2

Molecular weight: 106.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5QVU5|A0A0Q5QVU5_9SPHN Calpastatin OS=Sphingomonas sp. Leaf357 OX=1736350 GN=ASG11_04265 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.36GFRR19 pKa = 11.84ARR21 pKa = 11.84SATPGGRR28 pKa = 11.84NVLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.0KK41 pKa = 10.58LSAA44 pKa = 4.03

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3524

0

3524

1120184

29

1936

317.9

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.7 ± 0.069

0.728 ± 0.012

6.094 ± 0.03

4.883 ± 0.037

3.545 ± 0.028

9.072 ± 0.035

1.899 ± 0.02

5.162 ± 0.026

3.041 ± 0.031

9.689 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.021

2.531 ± 0.031

5.32 ± 0.029

2.933 ± 0.022

7.173 ± 0.04

5.093 ± 0.025

5.687 ± 0.036

7.388 ± 0.033

1.405 ± 0.019

2.257 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski