Canis familiaris papillomavirus 3
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q0QLX6|Q0QLX6_9PAPI Protein E6 OS=Canis familiaris papillomavirus 3 OX=360397 GN=E6 PE=3 SV=1
MM1 pKa = 7.39 IGKK4 pKa = 9.27 DD5 pKa = 2.95 ATLRR9 pKa = 11.84 DD10 pKa = 3.81 IVLEE14 pKa = 4.05 EE15 pKa = 4.16 QPTPVDD21 pKa = 3.94 NLWCDD26 pKa = 3.44 EE27 pKa = 4.35 EE28 pKa = 5.06 LAQSDD33 pKa = 4.05 DD34 pKa = 4.24 DD35 pKa = 4.35 EE36 pKa = 6.01 EE37 pKa = 4.3 EE38 pKa = 4.25 ALTNQNLRR46 pKa = 11.84 PFRR49 pKa = 11.84 IQTSCGTCEE58 pKa = 3.73 RR59 pKa = 11.84 GIKK62 pKa = 9.84 IVVLCTQEE70 pKa = 5.49 GIHH73 pKa = 6.51 CLEE76 pKa = 4.29 TLLCRR81 pKa = 11.84 NTSLCCPTCAASYY94 pKa = 10.56 RR95 pKa = 11.84 FDD97 pKa = 3.44 HH98 pKa = 6.85 GGG100 pKa = 2.97
Molecular weight: 11.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.094
IPC2_protein 4.38
IPC_protein 4.279
Toseland 4.113
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.113
Rodwell 4.113
Grimsley 4.024
Solomon 4.228
Lehninger 4.177
Nozaki 4.342
DTASelect 4.495
Thurlkill 4.126
EMBOSS 4.126
Sillero 4.393
Patrickios 1.036
IPC_peptide 4.228
IPC2_peptide 4.38
IPC2.peptide.svr19 4.291
Protein with the highest isoelectric point:
>tr|Q0QLX6|Q0QLX6_9PAPI Protein E6 OS=Canis familiaris papillomavirus 3 OX=360397 GN=E6 PE=3 SV=1
MM1 pKa = 7.26 EE2 pKa = 5.56 RR3 pKa = 11.84 PWSLASLCVRR13 pKa = 11.84 SGVSLCAISIPCVFCGNLLSFEE35 pKa = 4.72 DD36 pKa = 3.64 RR37 pKa = 11.84 VSFDD41 pKa = 3.47 FKK43 pKa = 11.07 VLQVSWKK50 pKa = 9.96 NGLPHH55 pKa = 7.31 ACCTSCARR63 pKa = 11.84 SICSRR68 pKa = 11.84 EE69 pKa = 3.71 VSQYY73 pKa = 8.1 TSEE76 pKa = 4.37 EE77 pKa = 3.15 ISYY80 pKa = 9.75 RR81 pKa = 11.84 QFVQRR86 pKa = 11.84 VGQGFYY92 pKa = 9.83 WIPVRR97 pKa = 11.84 CTCCLGLVTTTQKK110 pKa = 10.19 IQALYY115 pKa = 10.26 RR116 pKa = 11.84 RR117 pKa = 11.84 QKK119 pKa = 8.86 FKK121 pKa = 10.86 KK122 pKa = 9.85 VRR124 pKa = 11.84 GRR126 pKa = 11.84 WRR128 pKa = 11.84 TLCTYY133 pKa = 10.29 CAEE136 pKa = 4.75 SDD138 pKa = 3.78 NDD140 pKa = 3.26 WEE142 pKa = 3.82 RR143 pKa = 11.84 RR144 pKa = 11.84 YY145 pKa = 10.27 FEE147 pKa = 3.99 RR148 pKa = 11.84 HH149 pKa = 4.88 RR150 pKa = 11.84 SS151 pKa = 3.41
Molecular weight: 17.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.506
IPC2_protein 8.273
IPC_protein 8.126
Toseland 7.6
ProMoST 8.668
Dawson 8.785
Bjellqvist 9.238
Wikipedia 8.712
Rodwell 8.814
Grimsley 7.497
Solomon 8.887
Lehninger 8.902
Nozaki 9.516
DTASelect 8.887
Thurlkill 8.946
EMBOSS 8.99
Sillero 9.297
Patrickios 3.999
IPC_peptide 8.887
IPC2_peptide 8.902
IPC2.peptide.svr19 8.963
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2367
100
628
394.5
43.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.337 ± 0.609
2.07 ± 0.821
5.746 ± 0.649
6.126 ± 0.473
3.845 ± 0.567
7.52 ± 0.903
2.324 ± 0.254
3.929 ± 0.512
4.098 ± 0.701
8.407 ± 0.384
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.521 ± 0.346
3.971 ± 0.247
6.464 ± 1.075
4.183 ± 0.629
7.224 ± 0.427
8.323 ± 0.522
6.591 ± 0.315
6.971 ± 0.991
1.521 ± 0.329
2.831 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here