Dissulfuribacter thermophilus
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2462 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B9F8E9|A0A1B9F8E9_9DELT Release factor glutamine methyltransferase OS=Dissulfuribacter thermophilus OX=1156395 GN=prmC PE=3 SV=1
MM1 pKa = 7.66 AFLMDD6 pKa = 5.29 KK7 pKa = 10.21 INRR10 pKa = 11.84 PITEE14 pKa = 4.13 EE15 pKa = 3.86 EE16 pKa = 4.06 LAEE19 pKa = 5.25 LDD21 pKa = 4.83 DD22 pKa = 5.38 FLNSDD27 pKa = 3.96 QVPEE31 pKa = 4.18 EE32 pKa = 4.17 CLDD35 pKa = 3.56 LSGLHH40 pKa = 6.42 GFLTAITIGPGYY52 pKa = 10.21 IMPSEE57 pKa = 4.23 WLPIAIGLVEE67 pKa = 5.49 DD68 pKa = 4.75 DD69 pKa = 3.91 VEE71 pKa = 4.39 EE72 pKa = 4.48 NYY74 pKa = 10.76 SEE76 pKa = 4.05 GRR78 pKa = 11.84 EE79 pKa = 4.0 DD80 pKa = 3.65 SLEE83 pKa = 4.65 LIMRR87 pKa = 11.84 MYY89 pKa = 10.11 TGIVSEE95 pKa = 5.05 FYY97 pKa = 10.56 EE98 pKa = 5.03 DD99 pKa = 3.86 PDD101 pKa = 3.59 NFEE104 pKa = 3.92 PLIYY108 pKa = 10.37 FLEE111 pKa = 4.65 FNGKK115 pKa = 8.81 SFPIISDD122 pKa = 3.09 WCYY125 pKa = 9.86 GFVRR129 pKa = 11.84 GMSLRR134 pKa = 11.84 MDD136 pKa = 3.22 SWKK139 pKa = 11.26 SMMEE143 pKa = 4.04 SEE145 pKa = 4.72 DD146 pKa = 3.4 ASLFLTIPLLFGTEE160 pKa = 3.71 EE161 pKa = 4.15 GLQEE165 pKa = 4.1 LEE167 pKa = 4.95 AYY169 pKa = 8.99 TPDD172 pKa = 4.02 EE173 pKa = 4.01 IAHH176 pKa = 6.21 ISEE179 pKa = 4.17 GLPYY183 pKa = 10.17 SVLGIYY189 pKa = 8.74 EE190 pKa = 3.75 YY191 pKa = 10.45 WLNRR195 pKa = 11.84 KK196 pKa = 9.29 APTPLINN203 pKa = 3.85
Molecular weight: 23.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.656
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.668
Rodwell 3.668
Grimsley 3.567
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.037
Thurlkill 3.668
EMBOSS 3.681
Sillero 3.935
Patrickios 1.1
IPC_peptide 3.783
IPC2_peptide 3.923
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A1B9F8X4|A0A1B9F8X4_9DELT Uncharacterized protein OS=Dissulfuribacter thermophilus OX=1156395 GN=DBT_0111 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNIKK11 pKa = 10.16 RR12 pKa = 11.84 KK13 pKa = 8.1 RR14 pKa = 11.84 THH16 pKa = 6.08 GFRR19 pKa = 11.84 KK20 pKa = 9.95 RR21 pKa = 11.84 MSTRR25 pKa = 11.84 AGRR28 pKa = 11.84 KK29 pKa = 7.73 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.03 GRR39 pKa = 11.84 HH40 pKa = 5.34 RR41 pKa = 11.84 LTVV44 pKa = 3.07
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2462
0
2462
750110
37
1764
304.7
34.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.917 ± 0.046
1.316 ± 0.025
5.264 ± 0.037
7.227 ± 0.056
4.568 ± 0.037
7.42 ± 0.047
1.966 ± 0.023
7.674 ± 0.039
7.096 ± 0.054
10.29 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.252 ± 0.023
3.583 ± 0.033
4.517 ± 0.031
2.892 ± 0.03
5.561 ± 0.043
5.877 ± 0.037
4.707 ± 0.032
6.782 ± 0.046
1.088 ± 0.018
3.002 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here