Amphiprion percula (Orange clownfish) (Lutjanus percula)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei;

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33681 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P8RPH5|A0A3P8RPH5_AMPPE DDE Tnp4 domain-containing protein OS=Amphiprion percula OX=161767 PE=3 SV=1
MM1 pKa = 7.66LCTPSILDD9 pKa = 3.84HH10 pKa = 6.87LPSPMYY16 pKa = 9.47TFSVTAEE23 pKa = 4.12EE24 pKa = 4.22EE25 pKa = 4.04QGLDD29 pKa = 3.69GMDD32 pKa = 3.03WLDD35 pKa = 3.58FTSGRR40 pKa = 11.84EE41 pKa = 3.94KK42 pKa = 11.09DD43 pKa = 3.85EE44 pKa = 4.53EE45 pKa = 4.55PPTLAPLAPQTPSSVFSTDD64 pKa = 3.55FLDD67 pKa = 6.07GYY69 pKa = 10.27DD70 pKa = 3.65LHH72 pKa = 8.36IHH74 pKa = 6.97LDD76 pKa = 3.51SCLL79 pKa = 3.42

Molecular weight: 8.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P8SDX3|A0A3P8SDX3_AMPPE EFR3 homolog B OS=Amphiprion percula OX=161767 GN=EFR3B PE=4 SV=1
FF1 pKa = 7.61LKK3 pKa = 10.08LQNMSHH9 pKa = 6.35SKK11 pKa = 10.2QLLAHH16 pKa = 6.37FRR18 pKa = 11.84QLLAHH23 pKa = 6.61FRR25 pKa = 11.84QLLLLAHH32 pKa = 6.52FRR34 pKa = 11.84QLLAHH39 pKa = 6.75FKK41 pKa = 10.55QLLAHH46 pKa = 6.49FRR48 pKa = 11.84QLLAHH53 pKa = 7.02FGQQLVAHH61 pKa = 6.57FRR63 pKa = 11.84QLLAHH68 pKa = 6.51FRR70 pKa = 11.84QLLAHH75 pKa = 6.41FRR77 pKa = 11.84QLVAHH82 pKa = 6.49FRR84 pKa = 11.84QLVAHH89 pKa = 6.68FRR91 pKa = 11.84QLLGSSSSARR101 pKa = 11.84PASASFLPLPVLPAAVRR118 pKa = 11.84SRR120 pKa = 11.84PGLPPAVV127 pKa = 3.61

Molecular weight: 14.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23736

9945

33681

18269780

17

6189

542.4

60.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.51 ± 0.011

2.295 ± 0.01

5.216 ± 0.009

6.776 ± 0.019

3.702 ± 0.01

6.267 ± 0.016

2.671 ± 0.007

4.417 ± 0.011

5.604 ± 0.015

9.55 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.393 ± 0.006

3.84 ± 0.009

5.668 ± 0.018

4.736 ± 0.012

5.627 ± 0.012

8.721 ± 0.018

5.661 ± 0.01

6.399 ± 0.011

1.169 ± 0.004

2.778 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski