Boseongicola sp. HY14

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Muriiphilus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3383 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D0R9Y2|A0A5D0R9Y2_9RHOB Proline iminopeptidase OS=Boseongicola sp. HY14 OX=2603819 GN=pip PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.3KK3 pKa = 9.69ILLASTILVGTAGFAAADD21 pKa = 3.84NNNFTWSGEE30 pKa = 3.94AYY32 pKa = 10.35AGVAWSTTSGFAPEE46 pKa = 4.0VTASFTAGMATTTDD60 pKa = 3.36GGLEE64 pKa = 3.86AGAKK68 pKa = 9.63IKK70 pKa = 10.27IKK72 pKa = 10.97AMGVSMNKK80 pKa = 9.75DD81 pKa = 3.02HH82 pKa = 7.33TDD84 pKa = 3.17GGFGTVTANGTTVPPEE100 pKa = 3.97GTISDD105 pKa = 3.56ASVYY109 pKa = 10.77LSGDD113 pKa = 3.2FGKK116 pKa = 11.03FEE118 pKa = 4.24VVYY121 pKa = 10.69DD122 pKa = 3.99ADD124 pKa = 3.96GASATTYY131 pKa = 10.76DD132 pKa = 3.99VNFKK136 pKa = 10.88YY137 pKa = 11.06SNTWGDD143 pKa = 3.57FTVAAYY149 pKa = 7.5YY150 pKa = 10.14TYY152 pKa = 10.81DD153 pKa = 3.4PSGTMTNGDD162 pKa = 3.75MGVKK166 pKa = 10.28LSYY169 pKa = 11.66SMADD173 pKa = 2.97YY174 pKa = 9.92TFYY177 pKa = 11.34VEE179 pKa = 4.72PEE181 pKa = 3.5WDD183 pKa = 3.72ASVTDD188 pKa = 3.64YY189 pKa = 11.3KK190 pKa = 10.89IGFGASATMSGFTGAVDD207 pKa = 4.69LDD209 pKa = 4.21YY210 pKa = 11.77EE211 pKa = 4.32NATTDD216 pKa = 3.6WDD218 pKa = 3.4WKK220 pKa = 10.94ASVKK224 pKa = 10.52YY225 pKa = 9.15ATGPYY230 pKa = 9.6SVGAFVEE237 pKa = 4.27DD238 pKa = 5.34DD239 pKa = 4.02GVDD242 pKa = 3.4DD243 pKa = 4.99QMDD246 pKa = 3.5YY247 pKa = 10.95GANVGYY253 pKa = 10.42DD254 pKa = 3.24LGGGVSLDD262 pKa = 5.12AEE264 pKa = 4.76YY265 pKa = 10.67KK266 pKa = 9.71WDD268 pKa = 3.88ADD270 pKa = 3.86TTGSIFAVGVSMKK283 pKa = 10.37FF284 pKa = 3.11

Molecular weight: 29.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D0RH27|A0A5D0RH27_9RHOB SDR family oxidoreductase OS=Boseongicola sp. HY14 OX=2603819 GN=FVF75_10680 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3383

0

3383

1054027

28

2478

311.6

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.061 ± 0.06

0.787 ± 0.011

6.281 ± 0.036

5.955 ± 0.04

3.689 ± 0.029

8.992 ± 0.043

2.066 ± 0.019

5.042 ± 0.028

2.884 ± 0.034

9.899 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.766 ± 0.019

2.323 ± 0.021

5.291 ± 0.031

2.73 ± 0.019

7.182 ± 0.041

4.653 ± 0.025

5.369 ± 0.023

7.508 ± 0.035

1.392 ± 0.019

2.13 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski